The electrofusion of cells

Pohl, Herbert A.; Pollock, Kent; Rivera, Hiram

doi:10.1002/qua.560260734

Cited by 17 publications

(10 citation statements)

References 15 publications

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“…The latter two results (b and c) are incompatible with a fusion mechanism that proposes a simple relationship between electric field-induced pores and fusion. M EMBRANE fusion induced by electric fields (2,3,15,26,27) offers several unique characteristics both for studies of fusion mechanisms and for applications in which delivery of membrane contents or entrapped loads to a target is a component of the experiment. These characteristics include the possibility of very high fusion yields (2,3,15,26,27), control of the moment of fusion to small fractions of a second (2,3,15,19,26,27), fusion synchrony which is instantaneous as far as human perception can determine (19), and fusion induction without the use of exogenous chemicals.…”

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confidence: 99%

A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.

Sowers¹

1986

The Journal of Cell Biology

146

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Abstract. Treatment of erythrocyte ghosts in random positions in a suspension with membrane fusion-inducing direct current electric field pulses causes the membranes to become fusogenic. Significant fusion yields are observed if the membranes are dielectrophoretically aligned into m e m b r a n e -m e m b r a n e contact with a weak alternating electric field as much as 5 min after the application of the pulses. This demonstrates that a long-lived membrane structural alteration is involved in this fusion mechanism. Other experiments indicate that the areas on the membrane which become fusogenic after treatment with the pulses may be very highly localized. The locations of these fusogenic areas coincide with where the transmembrane electric field strength was greatest during the pulse. The fusogenic membrane alteration, or components thereof, in these areas laterally diffuses very slowly or not at all, or, to be fusogenic, must be present at concentrations in the membrane above a certain threshold. The loss of soluble 0.9-3-nm-diameter fluorescent probes from resealed cytoplasmic compartments of randomly positioned erythrocyte ghosts occurs through electric field pulse-induced pores only during a pulse but not between pulses or after a train of pulses if the probe diameter is 1.2 nm or greater. For a given pulse treatment of membranes in random positions in suspensions, an increase in ionic strength of the medium results in (a) a decrease in loss during the pulse, (b) no difference in loss between pulses, and (c) an increase in fusion yield when membrane-membrane contact is established. The latter two results (b and c) are incompatible with a fusion mechanism that proposes a simple relationship between electric field-induced pores and fusion.

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confidence: 99%

A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.

Sowers¹

1986

The Journal of Cell Biology

146

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“…In the early 1980s, Pohl et al [ 68 , 69 ] started using the eDEP system ( Figure 3 a) for cell-to-cell or cell-to-particle fusion and separation experiments in high throughput genetic engineering applications. The experiments resulted in the chain or pearl formation of cells by applying AC electric fields on parallel wire electrodes, eventually leading to cell membrane puncture or damage as cells come into direct contact with the electrodes.…”

Section: Discussionmentioning

confidence: 99%

“… eDEP and iDEP concept sketch. ( a ) Figure showing side view concept sketch of eDEP showing pearl chain formation of cell in a higher magnitude of electric field strength [ 68 ]. ( b ) Figure showing side view concept sketch of iDEP field constriction system used in fluid integrated circuit (FIC) for cell fusion experiments [ 36 ].…”

Section: Figurementioning

confidence: 99%

Insulator Based Dielectrophoresis: Micro, Nano, and Molecular Scale Biological Applications

Benhal

Quashie

Kim

et al. 2020

Sensors

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Insulator based dielectrophoresis (iDEP) is becoming increasingly important in emerging biomolecular applications, including particle purification, fractionation, and separation. Compared to conventional electrode-based dielectrophoresis (eDEP) techniques, iDEP has been demonstrated to have a higher degree of selectivity of biological samples while also being less biologically intrusive. Over the past two decades, substantial technological advances have been made, enabling iDEP to be applied from micro, to nano and molecular scales. Soft particles, including cell organelles, viruses, proteins, and nucleic acids, have been manipulated using iDEP, enabling the exploration of subnanometer biological interactions. Recent investigations using this technique have demonstrated a wide range of applications, including biomarker screening, protein folding analysis, and molecular sensing. Here, we review current state-of-art research on iDEP systems and highlight potential future work.

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“…A primary challenge in constructing cell-based devices is isolation and precise positioning of a controlled number of osteoblasts on a microdevice. Cell positioning by dielectrophoresis is probably the most broadly applied method among all chip-based cell separation technologies (Führ and Wangner, 1994;Shirley, 1995, 1998;Lee and Tai, 1999;Jones and Kallio, 1979;Pollack, 1979a, 1979b;Pohl et al, 1984;Porschke, 1985;Sato et al, 1990;Washizu, 1990;Wilkinson, 1998;Zimmermann, 1982a;Zimmermann and Pilwat, 1982b).…”

Section: Introductionmentioning

confidence: 99%

2-dimensional MEMS dielectrophoresis device for osteoblast cell stimulation

Zou

Mellon

Syms

et al. 2006

Biomed Microdevices

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A fixed microelectrode device for cell stimulation has been designed and fabricated using micro-electro-mechanical systems (MEMS) technology. Dielectrophoretic forces obtained from non-uniform electric fields were used for manipulating and positioning osteoblasts. The experiments show that the osteoblasts experience positive dielectrophoresis (p-DEP) when suspended in iso-osmotic culture medium and exposed to AC fields at 5 MHz frequency. Negative dielectrophoresis (n-DEP) is obtained at 0.1 MHz. The viability of osteoblasts under dielectrophoresis has been investigated. The viability values for cells exposed to DEP are nearly three times higher than the control values, indicating that dielectrophoresis may have an anabolic effect on osteoblasts.

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The electrofusion of cells

Cited by 17 publications

References 15 publications

A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.

A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.

Insulator Based Dielectrophoresis: Micro, Nano, and Molecular Scale Biological Applications

2-dimensional MEMS dielectrophoresis device for osteoblast cell stimulation

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