The Elimination of Hydroxyethyl Starch from the Blood of Donors Experiencing Single or Multiple Intermittent‐Flow Centrifugation Leukapheresis

This study was initiated in order to assess any immunologic effects that source leukocyte concentrate collection might have on double-bag plasmapheresis donors. Previous studies have shown that surveillance parameters, such as T versus non-T lymphocyte subpopulations, showed no abnormal values in donors with as many as 500 visits over a 12-year period. The present study demonstrates that the frequency and the total number of leukocyte donations do not effect the lymphocyte subpopulations and functions observed. No significant changes were noted for specific and nonspecific stimulation, natural killer (NK) cell activity, lymphocyte surface markers and a variety of functional parameters. For example, the apheresis donors manifested no differences in NK cell activity, human leukocyte interferon production, IgG synthesis by B cells and percent suppression of both IgG synthesis and mixed lymphocyte cultures, when compared to non-apheresed donors.

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“…Only when donors received HES were T-cell func tions lowered or impaired. The mechanism or cause of the HES effect is unknown [9].…”

Section: Discussionmentioning

confidence: 99%

Effects of Source Leukocyte Collection on the Immune System

Orsini¹,

Fitzpatrick²,

Cohen³

et al. 1982

Vox Sanguinis

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“…Prednisone was given orally either as a single dose (50 mg) about 12 h before leukapheresis, or in three divid ed doses (20 mg each) 15, 12 and 2 h before the procedure. suits in serious side-effects, temporary ab normalities in some blood chemistries [12,13], coagulation parameters [23], and plasma volume expansion after multiple transfu sions have been reported [12]. Recent studies have shown that a fraction of the infused HES is eliminated very slowly within the course of 1 year [13,14], generating uncer tainty about the safety of administering HES to leukapheresis donors repetitively.…”

Section: Resultsmentioning

confidence: 99%

“…The average increase of serum polysac charide concentration following the low HES leukapheresis procedure was about 100 mg/dl, which is about 20% of the values reported after the standard leukapheresis procedure [12][13][14], It should be noted that although the donor received a low dose of HES, the amount of HES added to the RBC during the separation was not less than that given during the standard leukapheresis pro cedure. Even multiple procedures resulted in a lower exposure of the donor to HES than one standard leukapheresis procedure, as is shown in a case where three procedures were performed in the course of 8 days ( fig.…”

Section: Discussionmentioning

confidence: 97%

“…The gran ulocytes can then be separated from red crease donors' plasma volume [12], limiting the number of procedures that one donor can undergo in a short period of time. Fur thermore, recent studies have shown the per sistence of small amounts of HES in donors' serum for up to 1 year after a single leukapheresis procedure [13,14]. At this time, the long-term effects of HES are unknown.…”

Section: Introductionmentioning

confidence: 99%

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A New Method to Collect Granulocytes Using a Low Dose of Hydroxyethyl Starch

Szymanski¹

1983

Vox Sanguinis

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A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average efficiency of granulocyte collection by this method is about 64%. The average yield of WBC from 2,600 ml of donors’ whole blood is 1.64 ± 0.54 x 10^10 (mean ± 1 SD) and the average granulocyte yield is 1.33 ± 0.51 x 10^10 (mean ± 1SD). The granulocytes were shown to migrate to the sites of infection in vivo. This approach is an improvement over standard discontinuous flow leukapheresis because of the high efficiency of granulocyte collection and because multiple procedures are possible without exposing donors to more HES than is currently given during a single procedure.

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“…We have previously reported a low-dose HES leukapheresis technique with good granulocyte yields [1] in which the amount of HES given to the donor is about 20% of that administered during standard discontin uous-flow leukapheresis [2,3].…”

Section: Introductionmentioning

confidence: 99%

Studies on Low-Dose Hydroxyethyl Starch Leukapheresis

Szymanski¹,

Teno²,

Gandhi³

et al. 1984

Vox Sanguinis

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We studied the rate of in vivo elimination of hydroxyethyl starch (HES) given during low dose HES leukapheresis in 9 donors and the effect of this procedure on the in vitro function of granulocytes in 3 donors. HES was eliminated more rapidly than has been previously reported for standard leukapheresis. Serum HES declined to one-half of peak concentration between 1 and 2 days and to one-tenth of peak in 23 days. No HES could be detected in serum 90 days after leukapheresis. The function of the harvested granulocytes was compared to that of granulocytes collected just prior to the procedure by measuring superoxide generation, ingestion and cell motility. There was no significant difference in the function of granulocytes harvested by low dose HES leukapheresis compared to those collected by venipuncture before the procedure.

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The Elimination of Hydroxyethyl Starch from the Blood of Donors Experiencing Single or Multiple Intermittent‐Flow Centrifugation Leukapheresis

Cited by 55 publications

References 17 publications

Effects of Source Leukocyte Collection on the Immune System

Effects of Source Leukocyte Collection on the Immune System

A New Method to Collect Granulocytes Using a Low Dose of Hydroxyethyl Starch

Studies on Low-Dose Hydroxyethyl Starch Leukapheresis

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