The emerging power and promise of non-coding RNAs in chronic pain

Zhang, Changteng; Gao, Rui; Zhou, Ruihao; Chen, Hai; Liu, Changliang; Zhu, Tao; Chen, Chan

doi:10.3389/fnmol.2022.1037929

Cited by 9 publications

(4 citation statements)

References 198 publications

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“…33 This burgeoning body of evidence underscores the potential utility of lncRNAs as both diagnostic biomarkers and promising therapeutic targets within the domain of cancer pain management. 27,34 In light of our research group's comprehensive transcriptome sequencing endeavors, we successfully identified lncRNA51325. This particular lncRNA, notable for its robust evolutionary conservation, had hitherto remained uncharted in the annals of scientific inquiry.…”

Section: Discussionmentioning

confidence: 99%

LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2

Wang,

Xu,

Liu

et al. 2024

JPR

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Background: Bone cancer pain (BCP) represents one of the most challenging comorbidities associated with cancer metastasis. Long non-coding RNAs (lncRNAs) have garnered attention as potential therapeutic agents in managing neuropathic pain. However, their role in the regulation of nociceptive information processing remains poorly understood. In this study, we observed a significant downregulation of the spinal lncRNA ENSRNOG00000051325 (lncRNA51325) in a rat model of bone cancer pain. Our study sought to elucidate the potential involvement of lncRNA51325 in the development of BCP by modulating the expression of molecules associated with pain modulation. Methods: We established the BCP model by injecting Walker 256 cells into the tibial plateau of rats. We conducted tests on the pain behaviors and anxiety-like responses of rats through von-Frey test, Gait analysis, and Open Field Test. Spinal lumbar expansion was harvested for molecular biology experiments to explore the relationship between lncRNA51325 and Pumilio RNA binding family member 2 (Pum2). Results: Notably, the overexpression of lncRNA51325 effectively attenuated mechanical allodynia in rats afflicted with BCP, whereas the knockdown of lncRNA51325 induced pain behaviors and anxiety-like responses in naïve rats. Additionally, we observed a timedependent increase in the expression of Pum2 in BCP-afflicted rats, and intrathecal injection of Pum2-siRNA alleviated hyperalgesia. Furthermore, our investigations revealed that lncRNA51325 exerts a negative modulatory effect on Pum2 expression. The overexpression of lncRNA51325 significantly suppressed Pum2 expression in BCP rats, while the knockdown of lncRNA51325 led to elevated Pum2 protein levels in the spinal cord of naïve rats. Subsequent treatment with Pum2-siRNA mitigated the downregulation of lncRNA51325-induced mechanical allodynia in naïve rats. Conclusion:Our findings indicate that lncRNA51325 plays a role in regulating bone cancer pain by inhibiting Pum2 expression, offering a promising avenue for novel treatments targeting nociceptive hypersensitivity induced by bone metastatic cancer.

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Section: Discussionmentioning

confidence: 99%

LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2

Wang,

Xu,

Liu

et al. 2024

JPR

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“…With more than 200 nucleotides, scholars have found that lncRNAs are key regulators of neuronal function, especially in the NP. Nearly 40% of lncRNAs are found only in the nervous system and are considered important in regulating gene expression ( Zhang et al, 2018 ; Li et al, 2019 ; Wu et al, 2019 ; Zhang C. T. et al, 2022 ). Its biological and molecular mechanisms are diverse and complex, and it has been studied in various NP models, such as sciatic nerve ligation (SNL), CCI, and diabetic NP (DNP) ( Hu et al, 2021 ; Xu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Global research trends on epigenetics and neuropathic pain: A bibliometric analysis

Zhu

Zhong

Gong

et al. 2023

Front. Mol. Neurosci.

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ObjectiveNeuropathic pain (NP) is a common disease that manifests with pathological changes in the somatosensory system. In recent years, the interactions of NP with the epigenetic mechanism have been increasingly elucidated. However, only a few studies have used bibliometric tools to systematically analyze knowledge in this field. The objective of this study is to visually analyze the trends, hotspots, and frontiers in epigenetics and NP research by using a bibliometric method.MethodsStudies related to epigenetics and NP were searched from the Science Citation Index-Expanded of the Web of Science Core Collection database. Search time is from inception to November 30, 2022. No restrictions were placed on language. Only articles and reviews were included as document types. Data on institutions, countries, authors, journal distribution, and keywords were imported into CiteSpace software for visual analysis.ResultsA total of 867 publications met the inclusion criteria, which spanned the period from 2000 to 2022. Over the years, the number of publications and the frequency of citations exhibited a clear upward trend in general, reaching a peak in 2021. The major contributing countries in terms of the number of publications were China, the United States, and Japan. The top three institutions were Rutgers State University, Xuzhou Medical University, and Nanjing Medical University. Molecular Pain, Pain, and Journal of Neuroinflammation contributed significantly to the volume of issues. Among the top 10 authors in terms of the number of publications, Tao Yuan-Xiang contributed 30 entries, followed by Zhang Yi with 24 and Wu Shao-Gen with 20. On the basis of the burst and clusters of keywords, “DNA methylation,” “Circular RNA,” “acetylation,” “long non-coding RNA,” and “microglia” are global hotspots in the field.ConclusionThe bibliometric analysis indicates that the number of publications related to epigenetics and NP is exhibiting a rapid increase. Keyword analysis shows that “DNA methylation,” “Circular RNA,” “acetylation,” “long non-coding RNA” and “microglia” are the most interesting terms for researchers in the field. More rigorous clinical trials and additional studies that explore relevant mechanisms are required in the future.

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“…Meanwhile, 353 lncRNAs were identified as differentially expressed (Figure S1), and 86 DE-mRNAs were identified (Figure 2E,F). Since many studies have explored the contribution of lncR-NAs to neuropathic pain [18], we focused on the possible roles of circRNAs. explored the contribution of lncRNAs to neuropathic pain [18], we focused on the possi roles of circRNAs.…”

Section: Profile Of Differentially Expressed Genes In Cipnmentioning

confidence: 99%

“…Since many studies have explored the contribution of lncR-NAs to neuropathic pain [18], we focused on the possible roles of circRNAs. explored the contribution of lncRNAs to neuropathic pain [18], we focused on the possi roles of circRNAs.…”

Section: Profile Of Differentially Expressed Genes In Cipnmentioning

confidence: 99%

Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy

Cao

Wang

et al. 2023

CIMB

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Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and severe side effect of first-line chemotherapeutic agents. The association between circular RNAs (circRNAs) and CIPN remains unclear. In this study, CIPN models were constructed with Taxol, while 134 differentially expressed circRNAs, 353 differentially expressed long non-coding RNAs, and 86 differentially expressed messenger RNAs (mRNAs) were identified utilizing RNA sequencing. CircRNA-targeted microRNAs (miRNAs) were predicted using miRanda, and miRNA-targeted mRNAs were predicted using TargetScan and miRDB. The intersection of sequencing and mRNA prediction results was selected to establish the circRNA–miRNA–mRNA networks, which include 15 circRNAs, 18 miRNAs, and 11 mRNAs. Functional enrichment pathway analyses and immune infiltration analyses revealed that differentially expressed mRNAs were enriched in the immune system, especially in T cells, monocytes, and macrophages. Cdh1, Satb2, Fas, P2ry2, and Zfhx2 were further identified as hub genes and validated by RT-qPCR, correlating with macrophages, plasmacytoid dendritic cells, and central memory CD4 T cells in CIPN. Additionally, we predicted the associated diseases, 36 potential transcription factors (TFs), and 30 putative drugs for hub genes using the DisGeNET, TRRUST, and DGIdb databases, respectively. Our results indicated the crucial role of circRNAs, and the immune microenvironment played in CIPN, providing novel insights for further research.

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The emerging power and promise of non-coding RNAs in chronic pain

Cited by 9 publications

References 198 publications

LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2

LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2

Global research trends on epigenetics and neuropathic pain: A bibliometric analysis

Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy

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