The emerging role of microfluidics in multi-material 3D bioprinting

Richard, Cynthia; Neild, Adrian; Cadarso, Víctor J.

doi:10.1039/c9lc01184f

Cited by 75 publications

(53 citation statements)

References 107 publications

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“…Another option for curing bio‐inks in 3D printing, without temperature control, is to co‐inject the polymer with a solution of crosslinker using a mixing injector or a coaxial injector. [ 151 ] This method involves the design of bio‐inks that undergo crosslinking from a two‐part solution, with the additional requirement being a very rapid gelation reaction. For example, sodium‐alginate undergoes a very rapid gelation when combined with a solution of calcium chloride at the injector tip.…”

Section: Injectable Hydrogels For Bioprintingmentioning

confidence: 99%

Injectability of Biosynthetic Hydrogels: Consideration for Minimally Invasive Surgical Procedures and 3D Bioprinting

Birman

Seliktar

2021

Adv Funct Materials

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Injectable hydrogels are often preferred when designing carriers for cell therapy or developing new bio-ink formulations. Biosynthetic hydrogels, which are a class of materials made with a hybrid design strategy, can be advantageous for endowing injectability while maintaining biological activity of the material. The chemical modification required to make these gels injectable by specific crosslinking pathways can be challenging and also make the hydrogels inhospitable to cells. Therefore, most efforts to functionalize biosynthetic hydrogel precursors toward injectability in the presence of cells try to balance between chemical and biological functionality, in order to preserve cell compatibility while addressing the injectability design challenges. Accordingly, hydrogel crosslinking strategies have evolved to include the use of photoinitiated "click" chemistry or bio-orthogonal reactions with rapid gelation kinetics and minimal cyto-toxicity required when working with cell-compatible hydrogel systems. With many new injectable biosynthetic materials emerging, their impact in cell-based regenerative medicine and bioprinting is also becoming more apparent. This review covers the main strategies that are used to endow biosynthetic polymers with injectability through rapid, cyto-compatible physical or covalent crosslinking and the main considerations for using the resulting injectable hydrogels in cell therapy, tissue regeneration, and bioprinting.

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Section: Injectable Hydrogels For Bioprintingmentioning

confidence: 99%

Injectability of Biosynthetic Hydrogels: Consideration for Minimally Invasive Surgical Procedures and 3D Bioprinting

Birman

Seliktar

2021

Adv Funct Materials

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“…It is, however, worth noting that technological advancement in the fields of materials science, cellular therapy, and drug discovery can boost AM processes advancement toward the next generation of drug delivery scaffold development. For instance, the integration of nanotechnology (e.g., soft lithography), micro/nanofluid, and bioprinting is a promising approach to enhance the control of scaffold processing/structure/delivery ( Davoodi et al, 2020 ; Richard et al, 2020 ). Indeed, the formation of multiple emulsions within microfluidic devices may enable the fabrication of microparticles with multiple cores and drug/cell loading and delivery capability ( Omidi et al, 2020 ; Tomeh and Zhao, 2020 ; Moreira et al, 2021 ).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Review on Computer-Aided Design and Manufacturing of Drug Delivery Scaffolds for Cell Guidance and Tissue Regeneration

Salerno¹,

Netti

2021

Front. Bioeng. Biotechnol.

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In the last decade, additive manufacturing (AM) processes have updated the fields of biomaterials science and drug delivery as they promise to realize bioengineered multifunctional devices and implantable tissue engineering (TE) scaffolds virtually designed by using computer-aided design (CAD) models. However, the current technological gap between virtual scaffold design and practical AM processes makes it still challenging to realize scaffolds capable of encoding all structural and cell regulatory functions of the native extracellular matrix (ECM) of health and diseased tissues. Indeed, engineering porous scaffolds capable of sequestering and presenting even a complex array of biochemical and biophysical signals in a time- and space-regulated manner, require advanced automated platforms suitable of processing simultaneously biomaterials, cells, and biomolecules at nanometric-size scale. The aim of this work was to review the recent scientific literature about AM fabrication of drug delivery scaffolds for TE. This review focused on bioactive molecule loading into three-dimensional (3D) porous scaffolds, and their release effects on cell fate and tissue growth. We reviewed CAD-based strategies, such as bioprinting, to achieve passive and stimuli-responsive drug delivery scaffolds for TE and cancer precision medicine. Finally, we describe the authors’ perspective regarding the next generation of CAD techniques and the advantages of AM, microfluidic, and soft lithography integration for enhancing 3D porous scaffold bioactivation toward functional bioengineered tissues and organs.

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“…By contrast, the microfluidic technique provides a robust platform to generate tissue engineering building blocks with a series of structures (i.e., particles and fibers) [16] as well as a high throughput, attributed to the continuous production. Combined with the 3-D printing technique, complex scaffolds can be achieved [17][18][19]. In addition, microfluidic chips have shown great potential in the field of tissue or organ mimicking, such as cellular microenvironment simulation [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

et al. 2020

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In recent years, the microfluidic technique has been widely used in the field of tissue engineering. Possessing the advantages of large-scale integration and flexible manipulation, microfluidic devices may serve as the production line of building blocks and the microenvironment simulator in tissue engineering. Additionally, in microfluidic technique-assisted tissue engineering, various biomaterials are desired to fabricate the tissue mimicking or repairing structures (i.e., particles, fibers, and scaffolds). Among the materials, gelatin methacrylate (GelMA)-based hydrogels have shown great potential due to their biocompatibility and mechanical tenability. In this work, applications of GelMA hydrogels in microfluidic technique-assisted tissue engineering are reviewed mainly from two viewpoints: Serving as raw materials for microfluidic fabrication of building blocks in tissue engineering and the simulation units in microfluidic chip-based microenvironment-mimicking devices. In addition, challenges and outlooks of the exploration of GelMA hydrogels in tissue engineering applications are proposed.

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The emerging role of microfluidics in multi-material 3D bioprinting

Abstract: To assist the transition of 3D bioprinting technology from simple lab-based tissue fabrication, to fully functional and implantable organs, the technology must not only provide shape control, but also functional control.

Cited by 75 publications

References 107 publications

Injectability of Biosynthetic Hydrogels: Consideration for Minimally Invasive Surgical Procedures and 3D Bioprinting

Injectability of Biosynthetic Hydrogels: Consideration for Minimally Invasive Surgical Procedures and 3D Bioprinting

Review on Computer-Aided Design and Manufacturing of Drug Delivery Scaffolds for Cell Guidance and Tissue Regeneration

Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering

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