The Emerging Role of the Prokineticins and Homeobox Genes in the Vascularization of the Placenta: Physiological and Pathological Aspects

Alfaidy, Nadia; Brouillet, Sophie; Rajaraman, Gayathri; Kalionis, Bill; Hoffmann, P.; Raia-Barjat, Tiphaïne; Benharouga, Mohamed; Murthi, Padma

doi:10.3389/fphys.2020.591850

Cited by 13 publications

(7 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3e). EG-VEGF is upregulated by hypoxia and is known to be involved in physiological angiogenesis [71,72]. Additionally, IGFBP-2 promotes angiogenesis by upregulating VEGF expression [73], and IL-1β also plays an important role in angiogenesis as an angiogenic molecule [74].…”

Section: Fabrication and Characterization Of Hadsc Spheroid In Open A...mentioning

confidence: 99%

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

Jeon,

Kang,

Bhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Myocardial infarction (MI) is treated with stem cell transplantation using various methods. However, current methods, such as stem cell/spheroids injection, cell sheets, and cardiac patches have some limitations, such as low stem cell engraftment and poor therapeutic effects. Furthermore, these methods cause secondary damage due to injection and suturing to immobilize them in the heart, inducing side effects. Methods We fabricated human adipose-derived stem cell spheroids-laden three-dimensional (3D)-printed patches. The morphology, viability, and paracrine angiogenic effect of spheroids formed within 3D patches were analyzed in in vitro experiments. We synthesized thiolated gelatin and maleimide-conjugated gelatin as the polymers and fabricated a tissue adhesive biosealant using the polymers. The biocompatibility and biodegradability of the biosealant were evaluated using human dermal fibroblasts in vitro and the mouse subcutaneous models in vivo. In addition, the therapeutic effects of stem cell spheroid-laden 3D patches (S_3DP) with biosealant were evaluated using a rat MI model in vivo. Results The spheroids showed increased viability and expression of angiogenic factors compared to two-dimensional cultured cells. Our gelatin-based tissue adhesive biosealants were rapidly formed via a thiol-ene reaction and disulfide bond formation and revealed stronger tissue adhesiveness than commercial fibrin glue. Furthermore, we successfully applied S_3DP using a biosealant in a rat MI model without suturing in vivo, thereby improving cardiac function and reducing fibrosis of the heart. Conclusion We developed S_3DP with gelatin-based tissue adhesive biosealant to treat MI. This 3D patch has dual modules, such as open pockets to directly deliver the spheroids with their paracrine effects and closed pockets to improve the engraft rate by protecting the spheroid from harsh microenvironments. In summary, S_3DP and biosealant have excellent potential as advanced stem cell therapies with a sutureless approach to MI treatment.

show abstract

Section: Fabrication and Characterization Of Hadsc Spheroid In Open A...mentioning

confidence: 99%

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

Jeon,

Kang,

Bhang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Placental insufficiency is a general term for the impaired delivery of oxygen and nutrients to the fetus from the placenta (Wardinger and Ambati, 2022). This is commonly attributed to 1) insufficient remodeling of the maternal uterine vessels by invasive trophoblast cells, leading to reduced or intermittent blood flow to the placenta; and/or 2) reduced vascularization (initiation of blood vessels) or angiogenesis (vessel branching) within the placenta (Zygmunt et al, 2003;Burton and Jauniaux, 2004;Alfaidy et al, 2020). Placental malperfusion is also associated with abnormal pathology such as an increase in chorionic villus fibrosis, uteroplacental thrombosis, placental infarcts, and fibrin deposits (Burton and Jauniaux, 2004;Agarwal et al, 2017).…”

Section: The Inception Of Placental Genetics 21 the Nourishing Placentamentioning

confidence: 99%

Genetic variation in placental insufficiency: What have we learned over time?

Wang

Fernández-Boyano

Robinson

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Genetic variation shapes placental development and function, which has long been known to impact fetal growth and pregnancy outcomes such as miscarriage or maternal pre-eclampsia. Early epidemiology studies provided evidence of a strong heritable component to these conditions with both maternal and fetal-placental genetic factors contributing. Subsequently, cytogenetic studies of the placenta and the advent of prenatal diagnosis to detect chromosomal abnormalities provided direct evidence of the importance of spontaneously arising genetic variation in the placenta, such as trisomy and uniparental disomy, drawing inferences that remain relevant to this day. Candidate gene approaches highlighted the role of genetic variation in genes influencing immune interactions at the maternal-fetal interface and angiogenic factors. More recently, the emergence of molecular techniques and in particular high-throughput technologies such as Single-Nucleotide Polymorphism (SNP) arrays, has facilitated the discovery of copy number variation and study of SNP associations with conditions related to placental insufficiency. This review integrates past and more recent knowledge to provide important insights into the role of placental function on fetal and perinatal health, as well as into the mechanisms leading to genetic variation during development.

show abstract

“…Frontiers in Physiology frontiersin.org adverse consequences of FGR and its effects on the fetus caused by the aging process. The most common cause of FGR is placental insufficiency due to impaired placental blood flow and vascular abnormalities, (Alfaidy et al, 2014;Alfaidy et al, 2020), (Alfaidy et al, 2014;Alfaidy et al, 2020) which is similar to the pathogenesis of PE. Therefore, patients with PE are prone to FGR, which aggravates the original pathological state and causes the perinatal outcome of the mother and child to be worse than with PE or FGR alone.…”

Section: Groupsmentioning

confidence: 99%

Prediction of pre-eclampsia complicated by fetal growth restriction and its perinatal outcome based on an artificial neural network model

et al. 2022

View full text Add to dashboard Cite

Objective: Pre-eclampsia (PE) complicated by fetal growth restriction (FGR) increases both perinatal mortality and the incidence of preterm birth and neonatal asphyxia. Because ultrasound measurements are bone markers, soft tissues, such as fetal fat and muscle, are ignored, and the selection of section surface and the influence of fetal position can lead to estimation errors. The early detection of FGR is not easy, resulting in a relative delay in intervention. It is assumed that FGR complicated with PE can be predicted by laboratory and clinical indicators. The present study adopts an artificial neural network (ANN) to assess the effect and predictive value of changes in maternal peripheral blood parameters and clinical indicators on the perinatal outcomes in patients with PE complicated by FGR.Methods: This study used a retrospective case-control approach. The correlation between maternal peripheral blood parameters and perinatal outcomes in pregnant patients with PE complicated by FGR was retrospectively analyzed, and an ANN was constructed to assess the value of the changes in maternal blood parameters in predicting the occurrence of PE complicated by FGR and adverse perinatal outcomes.Results: A total of 15 factors—maternal age, pre-pregnancy body mass index, inflammatory markers (neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio), coagulation parameters (prothrombin time and thrombin time), lipid parameters (high-density lipoprotein, low-density lipoprotein, and triglyceride counts), platelet parameters (mean platelet volume and plateletcrit), uric acid, lactate dehydrogenase, and total bile acids—were correlated with PE complicated by FGR. A total of six ANNs were constructed with the adoption of these parameters. The accuracy, sensitivity, and specificity of predicting the occurrence of the following diseases and adverse outcomes were respectively as follows: 84.3%, 97.7%, and 78% for PE complicated by FGR; 76.3%, 97.3%, and 68% for provider-initiated preterm births,; 81.9%, 97.2%, and 51% for predicting the severity of FGR; 80.3%, 92.9%, and 79% for premature rupture of membranes; 80.1%, 92.3%, and 79% for postpartum hemorrhage; and 77.6%, 92.3%, and 76% for fetal distress.Conclusion: An ANN model based on maternal peripheral blood parameters has a good predictive value for the occurrence of PE complicated by FGR and its adverse perinatal outcomes, such as the severity of FGR and preterm births in these patients.

show abstract

The Emerging Role of the Prokineticins and Homeobox Genes in the Vascularization of the Placenta: Physiological and Pathological Aspects

Cited by 13 publications

References 74 publications

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

Genetic variation in placental insufficiency: What have we learned over time?

Prediction of pre-eclampsia complicated by fetal growth restriction and its perinatal outcome based on an artificial neural network model

Contact Info

Product

Resources

About