The emerging roles of circular RNAs in CNS injuries

Qu, Xiaolin; Liu, Zhenxing; Chen, Jigang; Hou, Lingling

doi:10.1002/jnr.24591

Cited by 20 publications

(12 citation statements)

References 102 publications

(136 reference statements)

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“…CircRNAs can regulate the pathological process of various types of diseases, including ocular diseases [11][12][13]. However, the role of circRNAs in cataract is rarely reported, particularly in the regulation of Crybb2 knockout on circRNA expression in the lens.…”

Section: Discussionmentioning

confidence: 99%

RNA-seq analysis of circRNA profiles in the lens of Crybb2 knockout mice

Gao¹,

Xu²,

Duan³

et al. 2021

Preprint

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Background: Currently, there is little information on the expression profiles of circRNAs in the lens. βB2-crystallin (CRYBB2) is an abundant protein in the mammalian lens, and its abnormal expression contributes to the development of cataract. This study aimed at exploring how Crybb2 knockout could modulate the expression profiles of circRNAs in mouse lens. Methods and Results: We extracted total RNAs from the lens of wide-type (WT) and Crybb2 -/- mice and after depleted their rRNAs and broken the remaining RNAs, we reversely transcribed the RNAs into cDNAand sequenced them. Furthermore, we performed bioinformatics to identify and analyze the differentially expressed circRNAs and predicted their potential functions. We validated some differentially expressed circRNAs by quantitative RT-PCR. We employed RNA-seq to identify 49,494 circRNAs and compared to the WT lens, 149 circRNAs were upregulated and 172 downregulated in Crybb2 -/- mouse lens. With the top 300 miRNA-circRNA interaction pairs, we constructed a network of circRNA-miRNA interactions. Moreover, those differentially expressed circRNAs participated in various biological processes, such as lens fiber cell development, calcium channel complex, structural constituent of the lens. They were involved in several important pathways, such as the canonical Wnt signaling pathway. Quantitative RT-PCR validated some differently expressed circRNAs in the lens of Crybb2 -/- mice. Conclusions: Crybb2 knockout significantly modulated circRNA expression profiles in the lens of mice, which may help clarify the roles of circRNAs in age-related cataracts.

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Section: Discussionmentioning

confidence: 99%

RNA-seq analysis of circRNA profiles in the lens of Crybb2 knockout mice

Gao¹,

Xu²,

Duan³

et al. 2021

Preprint

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show abstract

“…Most circRNAs are generated from protein-coding genes by the reverse connection of the downstream 5′ splice site with the upstream 3′ splice site. Via the specific splicing, circRNAs are single-strained and blessed with covalently closed-loop structure for lack of 5′ and 3′ ends and poly(A) tail whose appearance is totally different from corresponding linear RNAs ( Figure 1A ) ( 13 , 14 ).…”

Section: Characteristics Of Circrnasmentioning

confidence: 99%

“…Due to tissue and cellular structure specificity of circRNAs, increasing studies have been revealed that circRNAs are connected to neurological dysfunctions caused by brain damage (13,51). Oligodendrocytes (OLs) and myelin have been demonstrated to downregulate in the course of WMD, along with functional loss of synaptic transmission, eventually leading to white matter atrophy (52)(53)(54).…”

Section: Circrnas As Potential Biomarkers For Neurological Impairments and Diseasesmentioning

confidence: 99%

A Narrative Review of Circular RNAs in Brain Development and Diseases of Preterm Infants

Liu

et al. 2021

Front. Pediatr.

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Circular RNAs (circRNAs) generated by back-splicing are the vital class of non-coding RNAs (ncRNAs). Circular RNAs are highly abundant and stable in eukaryotes, and many of them are evolutionarily conserved. They are blessed with higher expression in mammalian brains and could take part in the regulation of physiological and pathophysiological processes. In addition, premature birth is important in neurodevelopmental diseases. Brain damage in preterm infants may represent the main cause of long-term neurodevelopmental disorders in surviving babies. Until recently, more and more researches have been evidenced that circRNAs are involved in the pathogenesis of encephalopathy of premature. We aim at explaining neuroinflammation promoting the brain damage. In this review, we summarize the current findings of circRNAs properties, expression, and functions, as well as their significances in the neurodevelopmental impairments, white matter damage (WMD) and hypoxic-ischemic encephalopathy (HIE). So we think that circRNAs have a direct impact on neurodevelopment and brain injury, and will be a powerful tool in the repair of the injured immature brain. Even though their exact roles and mechanisms of gene regulation remain elusive, circRNAs have potential applications as diagnostic biomarkers for brain damage and the target for neuroprotective intervention.

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“…Circular RNAs (circRNAs) are endogenous single-stranded RNA molecules produced by circularization events (Guo et al, 2014 ; Wang et al, 2014 ), shown to be very stable and conserved functional molecules, highly expressed in the central nervous system (CNS; Memczak et al, 2013 ; Rybak-Wolf et al, 2015 ). circRNAs play crucial regulatory roles in gene expression at the posttranscriptional level (Zhang et al, 2013 ; Chen, 2020 ) and are closely associated with multiple neurodegenerative diseases, especially at the level of the neuro-immune pathways (Qu et al, 2020 ; Xu et al, 2021 ). Therefore, it is possible that circRNAs may also play important role in molecular events involved in spinal cord injury (SCI) pathology.…”

Section: Introductionmentioning

confidence: 99%

Circular RNAs: The Novel Actors in Pathophysiology of Spinal Cord Injury

Sámano

Mladinić

Mazzone

2021

Front. Integr. Neurosci.

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Spinal Cord Injury (SCI) can elicit a progressive loss of nerve cells promoting disability, morbidity, and even mortality. Despite different triggering mechanisms, a cascade of molecular events involving complex gene alterations and activation of the neuroimmune system influence either cell damage or repair. Effective therapies to avoid secondary mechanisms underlying SCI are still lacking. The recent progression in circular RNAs (circRNAs) research has drawn increasing attention and opened a new insight on SCI pathology. circRNAs differ from traditional linear RNAs and have emerged as the active elements to regulate gene expression as well as to facilitate the immune response involved in pathophysiology-related conditions. In this review, we focus on the impact and possible close relationship of circRNAs with pathophysiological mechanisms following SCI, where circRNAs could be the key transcriptional regulatory molecules to define neuronal death or survival. Advances in circRNAs research provide new insight on potential biomarkers and effective therapeutic targets for SCI patients.

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The emerging roles of circular RNAs in CNS injuries

Cited by 20 publications

References 102 publications

RNA-seq analysis of circRNA profiles in the lens of Crybb2 knockout mice

RNA-seq analysis of circRNA profiles in the lens of Crybb2 knockout mice

A Narrative Review of Circular RNAs in Brain Development and Diseases of Preterm Infants

Circular RNAs: The Novel Actors in Pathophysiology of Spinal Cord Injury

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