The EMT transcription factor Snai1 maintains myocardial wall integrity by repressing intermediate filament gene expression

Gentile, Alessandra; Bensimon-Brito, Anabela; Priya, Rashmi; Maischein, Hans-Martin; Piesker, Janett; Guenther, Stefan; Gunawan, Felix; Stainier, Didier Y.R.

doi:10.7554/elife.66143

Cited by 14 publications

(15 citation statements)

References 62 publications

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“…Our results highlight its major role at the tissue level where it impacts cell tension mainly at the CM interface by increasing cell contractility. In zebrafish, it was shown that invalidation of the snaib factor induced extrusion of cardiomyocytes with increased contractility and an alteration in the desmb gene (orthologue of the des gene in humans) ( Gentile et al, 2021 ). Overexpression of desmin recapitulates this process and highlights its role in maintaining the myocardial wall.…”

Section: Discussionmentioning

confidence: 99%

The importance of intermediate filaments in the shape maintenance of myoblast model tissues

Nagle

Delort

Hénon

et al. 2022

eLife

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Liquid and elastic behaviors of tissues drives their morphology and their response to the environment. They appear as the first insight into tissue mechanics. We explore the role of individual cell properties on spheroids of mouse muscle precursor cells and investigate the role of intermediate filaments on surface tension and Young's modulus. By attening multicellular myoblast aggregates under magnetic constraint, we measure their rigidity and surface tension and show that they act as highly sensitive macroscopic reporters closely related to microscopic local tension and effective adhesion. Shedding light on the major contributions of acto-myosin contractility, actin organization and intercellular adhesions, we reveal the role of a major component of intermediate filaments in the muscle, desmin and its organization, on the macroscopic mechanics of these tissue models. Implicated in the mechanical and shape integrity of cells, intermediate filaments are found to be crucial to the mechanics of unorganized muscle tissue models even at an early stage of differentiation both in terms of elasticity and surface tension.

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Section: Discussionmentioning

confidence: 99%

The importance of intermediate filaments in the shape maintenance of myoblast model tissues

Nagle

Delort

Hénon

et al. 2022

eLife

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“…4g). These genes are essential for myocardial differentiation, cardiac primordia migration, as well as cardiac valve and second heart field morphogenesis (Ellertsdottir et al 2012;Gentile et al 2021;Nevis et al 2013;Sam et al 2020;Schindler et al 2014). Especially the zinc finger transcription factors (gata4/5/6).…”

Section: Discussionmentioning

confidence: 99%

Effects of bepridil on early cardiac development of zebrafish

Wei

Lei

et al. 2022

Cell Tissue Res

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Bepridil is a commonly used medication for arrhythmia and heart failure. It primarily exerts hemodynamic effects by inhibiting Na + /K + movement and regulating the Na + /Ca 2+ exchange. In comparison to other Ca 2+ inhibitors, bepridil has a long half-life and a complex pharmacology. Additionally, it is widely used in antiviral research and the treatment of various diseases. However, the toxicity of this compound and its other possible effects on embryonic development are unknown. In this study, we investigated the toxicity of bepridil on rat myocardial H9c2 cells. After treatment with bepridil, the cells became overloaded with Ca 2+ and entered a state of cytoplasmic vacuolization and nuclear abnormality. Bepridil treatment resulted in several morphological abnormalities in zebrafish embryo models, including pericardium enlargement, yolk sac swelling, and growth stunting. The hemodynamic effects on fetal development resulted in abnormal cardiovascular circulation and myocardial weakness. After inhibiting the Ca 2+ transmembrane, the liver of zebrafish larvae also displayed an ectopic and deficient spatial location. Additionally, the results of the RNA-seq analysis revealed the detailed gene expression profiles and metabolic responses to bepridil treatment in zebrafish embryonic development. Taken together, our study provides an important evaluation of antiarrhythmic agents for clinical use in prenatal heart patients. Supplementary Information The online version contains supplementary material available at 10.1007/s00441-022-03706-w.

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“…The dataset used comprised HNSCC mRNA-seq data from TCGA. A heat map of the correlation between S100 family genes and EMT-related genes of SNAI1 ( 18 ), VIM ( 19 ), TWIST ( 19 ), CDH1 ( 20 ), CDH2 ( 20 ) were performed by the Spearman correlation via R software package ggstatsplot ( 21 ), and is displayed by the R software package pheatmap ( 21 ). A P value of less than 0.05 was considered statistically significant.…”

Section: Methodsmentioning

confidence: 99%

An integrated bioinformatics analysis of the S100 in head and neck squamous cell carcinoma

Guo¹,

Yue²,

You³

et al. 2023

Transl Cancer Res

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Background: This study aims to evaluate the expression profile and clinical value of the S100 family in head and neck squamous cell carcinoma (HNSCC). Methods:The expression patterns, clinicopathological features, prognostic significance and underlying correlations of S100 family genes in HNSCC were determined by bioinformatics analysis with the application of several databases, including the The Cancer Genome Atlas (TCGA) and Oncomine for differential expression gene (DEG) analysis, and a series of analysis tools, including Database for Annotation, Visualization and Integrated Discovery (DAVID), cBioPortal, Kaplan-Meier Plotter, Tumor Immune Estimation Resource (TIMER) and R software packages. Results:The results from the study demonstrated that S100A4, S100A10, and S100A13 may act as prognostic markers through overall survival (OS), disease-free survival (DFS) and tumor infiltrating immune cell enrichment and a prognostic S100 family gene model comprising S100A1-A4, S100A8, S100A10, S100A12, and S100A13 was identified. The messenger RNA (mRNA) expression of S100A1, S100A9, S100A14, and S100A7A was significantly different in HNSCC patients, together with a high mutation rate of the S100 family was found. Evaluation of clinicopathological value demonstrated the heterogeneity of S100 family functions. S100A1, S100A7, S100A8, S100A9, S100A13, S100A14, and S100A16 were observed to significantly correlate with multiple biological processes (BPs) of HNSCC, including initiation, lymph node metastasis, and lymphovascular invasion. In addition, the S100 family were significantly associated with epithelial-mesenchymal transition (EMT)-related genes.Conclusions: This present study demonstrated that S100 family members are implicated in the initiation, progression, metastasis and survival of HNSCC.

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The EMT transcription factor Snai1 maintains myocardial wall integrity by repressing intermediate filament gene expression

Cited by 14 publications

References 62 publications

The importance of intermediate filaments in the shape maintenance of myoblast model tissues

The importance of intermediate filaments in the shape maintenance of myoblast model tissues

Effects of bepridil on early cardiac development of zebrafish

An integrated bioinformatics analysis of the S100 in head and neck squamous cell carcinoma

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