The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis

Mehdi, Wesen Adel; Mehde, Atheer Awad; Yusof, Faridah; Raus, Raha Ahmad; Resen, Ali Khudhair; Ghazali, Hamid

doi:10.1016/j.ijbiomac.2019.08.184

Cited by 2 publications

(2 citation statements)

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“…GSTA3 has a protective effect on Tubular epithelial-mesenchymal transition in renal fibrosis ( Xiao et al, 2016 ). Glutathione S transferase M1 (GSTM1) is an important member of phase II toxic metabolizing enzyme GST family, which plays a detoxification role by binding with glutathione ( Mehdi et al, 2019 ). It is reported that GSTM1 can repair acute kidney injury by reducing endoplasmic reticulum and oxidative stress ( Dai et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

et al. 2023

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Introduction: Epimedium, a traditional Chinese medicine (TCM) commonly used in ancient and modern China, is one of the traditional Chinese medicines clinically used to treat kidney yang deficiency syndrome (KYDS). There are differences in the efficacy of Epimedium before and after processing, and the effect of warming the kidney and enhancing yang is significantly enhanced after heating with suet oil. However, the active compounds, corresponding targets, metabolic pathways, and synergistic mechanism of frying Epimedium in suet oil to promote yang, remain unclear.Methods: Herein, a strategy based on comprehensive GC-TOF/MS metabolomics and network pharmacology analysis was used to construct an “active compounds-targets-metabolic pathways” network to identify the active compounds, targets and metabolic pathways involved. Subsequently, the targets in kidney tissue were further validated by real-time quantitative polymerase chain reaction (RT-qPCR). Histopathological analysis with physical and biochemical parameters were performed.Results: Fifteen biomarkers from urine and plasma, involving five known metabolic pathways related to kidney yang deficiency were screened. The network pharmacology results showed 37 active compounds (13 from Epimedium and 24 from suet oil), 159 targets, and 267 pathways with significant correlation. Importantly, integrated metabolomics and network pharmacologic analysis revealed 13 active compounds (nine from Epimedium and four from suet oil), 7 corresponding targets (ALDH2, ARG2, GSTA3, GSTM1, GSTM2, HPGDS, and NOS2), two metabolic pathways (glutathione metabolism, arginine and proline metabolism), and two biomarkers (Ornithine and 5-Oxoproline) associated with improved kidney yang deficiency by Epimedium fried with suet oil.Discussion: These finds may elucidate the underlying mechanism of yang enhancement via kidney warming effects. Our study indicated that the mechanism of action mainly involved oxidative stress and amino acid metabolism. Here, we demonstrated the novel strategies of integrating metabolomics and network pharmacology in exploring of the mechanisms of traditional Chinese medicines.

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Section: Discussionmentioning

confidence: 99%

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

et al. 2023

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“…23 GSH has many important biological functions 24 and plays an important role in many biochemical processes, including redox activity, 25 signal transduction 26 and gene regulation. 27 Moreover, cell health is critically related to the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG). 28 For example, the ratio is affected by the conversion of glutathione to oxidized glutathione when oxidative stress occurs.…”

Section: Introductionmentioning

confidence: 99%

Controllable synthesis of NiCo₂O₄ nanoparticles supported on MnO₂ nanowires with excellent oxidase-mimicking performance

Chen,

Xie,

Gao

et al. 2024

CrystEngComm

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The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis

Cited by 2 publications

References 47 publications

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

Controllable synthesis of NiCo₂O₄ nanoparticles supported on MnO₂ nanowires with excellent oxidase-mimicking performance

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The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis

Cited by 2 publications

References 47 publications

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

Integrating strategies of metabolomics, network pharmacology, and experiment validation to investigate the processing mechanism of Epimedium fried with suet oil to warm kidney and enhance yang

Controllable synthesis of NiCo2O4 nanoparticles supported on MnO2 nanowires with excellent oxidase-mimicking performance

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Controllable synthesis of NiCo₂O₄ nanoparticles supported on MnO₂ nanowires with excellent oxidase-mimicking performance