The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs

Chen, Lei; Xiao, Zhifeng; Yue, Meng; Zhao, Yannan; Han, Jin Soo; Su, Guannan; Chen, Bing; Dai, Jianwu

doi:10.1016/j.biomaterials.2011.10.056

Cited by 258 publications

(225 citation statements)

References 46 publications

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“…collagen, matrigel, scaffolds) facilitating cell adhesion and aggregation. Other 3D systems use liquid overlay technologies, fibre meshwork made of biocompatible polymers, solid or porous beads or extracellular matrices and their substitutes and require the addition of artificially produced supplements for achieving 3D growing cell cultures [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Development of an innovative 3D cell culture system to study tumour - stroma interactions in non-small cell lung cancer cells

Amann¹,

Zwierzina²,

Gamerith³

et al. 2013

Pneumologie

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Section: Introductionmentioning

confidence: 99%

Development of an innovative 3D cell culture system to study tumour - stroma interactions in non-small cell lung cancer cells

Amann¹,

Zwierzina²,

Gamerith³

et al. 2013

Pneumologie

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“…44 Accumulating evidence also indicates that cancer cells in 3D culture display higher malignancy and invasive capability compared to their counterparts in two-dimensional (2D) culture, which has been linked to increased cancer cell stemness. 45 Consequently, a number of polymers have been utilized to develop 3D cell culture scaffolds for the investigation of proliferation, growth and migration of CSCs.…”

Section: Biomaterials For Csc Growthmentioning

confidence: 99%

“…Chen et al observed that a 3D collagen scaffold promoted overexpression of pro-angiogenic growth factors, enhanced the tumorigenicity of breast cancer cells in vivo, upregulated EMT markers, and enriched the CSC population. 45 Adenoid cystic carcinoma cells have also been cultured in collagen matrices with similar effects of increased angiogenesis and up-regulation of CSC and EMT markers. 57 In addition, the authors observed increased resistance to chemotherapeutic drugs when the cells were cultured in the 3D matrix.…”

Section: Biomaterials For Csc Growthmentioning

confidence: 99%

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

Ordikhani

Kim

Zustiak

2015

JOM

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The theory of cancer stem cells (CSCs) and their role in cancer metastasis, tumorigenicity and resistance to therapy is slowly shifting the emphasis on the search for cancer cure: more evidence is surfacing that a successful therapy should be geared against this rare cancer cell population. Unfortunately, CSCs are difficult to culture in vitro which severely limits the progress of CSC research. This review gives a brief overview of CSCs and their microenvironment, with particular focus on studies that used in vitro biomaterial-based models and biomaterial/CSC interfaces for the enrichment of CSCs. Biomaterial properties relevant to CSC behaviors are also addressed. While the discussed research field is still in its infancy, it appears that in vitro cancer models that include a biomaterial can support CSC enrichment and this has proved indispensable to the study of their biology as well as the development of novel cancer therapies.

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“…These results are consistent with previous reports describing that collagen not only promotes the proliferation of cancer cells but also regulates their aggressiveness, depending on the concentration and stiff ness of the ECM. [23,24] Moreover, the contrasting morphologies of SK OV 3 and SW620 cells in response to the ECM composi tion underline the importance of ECM selection in cancer models depending on the origin. We could quantify those results with dif ferent indexes, such as number of nuclei or cytoplasmic volume of each cell ( Figure 1D,E).…”

Section: Angiogenesismentioning

confidence: 99%

Biomimetic Model of Tumor Microenvironment on Microfluidic Platform

Chung

Ahn

Son

et al. 2017

Adv Healthcare Materials

114

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COMMUNICATION (1 of 7)© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Biomimetic Model of Tumor Microenvironment on Microfluidic PlatformMinhwan Chung, Jungho Ahn, Kyungmin Son, Sudong Kim, and Noo Li Jeon* DOI: 10.1002/adhm.201700196 "cure." [4,5] The TME is a complex, inter acting system including the tumor itself, other noncancerous cell types such as immune, stromal and endothelial cells, and the extracellular matrix surrounding these cells. [6,7] This complexity has largely prevented a comprehensive understanding of TME in conventional in vitro models, which have been too simple to recapitulate the intricate interactions ( Figure 1A). [8,9] During recent decades, microfabrication technologies have been shown to be valu able tools in the exploration of tumor pro gression. Recently, several studies using microfluidic platform have been reported that recreate the 3D context of each aspect of the TME and metastasis in vitro. [10][11][12][13] However, to our knowledge, no versatile in vitro experimental model that reconstitutes direct interplay between constituents of the TME during tumorigenesis has yet been reported. Here we describe a biomimetic TME model to mimic the complex inter actions of the tumor, stromal, and endothe lial cells, all of which are essential components of the TME that hinder the effective treatment of cancers. [14][15][16] We used a micro fluidic platform from our previous studies. [17,18] Various effects of extracellular matrix (ECM) and the stroma on the physiology of tumor cells and angiogenesis were tested. Finally, we could mimic simultaneous angiogenesis and lymphangiogenesis in the TME with interactions between the tumor cells.Although the cancer stroma is well known for its many roles in multiple cancer stages, direct interactions between cancer and stromal cells have remained largely unknown. [19] Addition ally, cancer cell interaction with the ECM is another TME factor that regulates the behavior of the cancer cells and their resist ance to drugs. [20] Recently, in vitro activation of tumor stoma and corresponding ECM remodeling have been reported using a microfluidic platform. [21] However, single cell responses of the cancer cells in response to the stroma coculture and ECM composition has not yet been described. To examine cancer stromal cell interaction with a 3D ECM, we used a micro fluidic 3D cell culture platform previously reported from our group. [17,18] The array of microposts in the platform enabled straightforward micropatterning of the hydrogel which allowed flexible experimental configurations. We first cocultured cancer and stromal cells within fibrin gel in different microchannels, which still allowed the cells to interact with each other in a par acrine manner ( Figure 1B). Twice as many fibroblasts as cancer cells were patterned to exclude an autocrine or indirect effectThe "Tumor microenvironment" (TME) is a complex, interacting system of the tumor and its surrounding environment. The TME has drawn more attention recently in attempts to overcome current drug...

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The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs

Cited by 258 publications

References 46 publications

Development of an innovative 3D cell culture system to study tumour - stroma interactions in non-small cell lung cancer cells

Development of an innovative 3D cell culture system to study tumour - stroma interactions in non-small cell lung cancer cells

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

Biomimetic Model of Tumor Microenvironment on Microfluidic Platform

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