The ENU-induced powdery mildew resistant mutant pea (Pisum sativum L.) lines S(er1mut1) and F(er1mut2) harbour early stop codons in the PsMLO1 gene

Santo, Tatiana; Rashkova, Maya; Alabaça, Cláudia S.; Leitão, José

doi:10.1007/s11032-013-9889-x

Cited by 21 publications

(25 citation statements)

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“…The resistance gene er1 is the most widely deployed gene controlling powdery mildew in pea cultivars worldwide. Furthermore, er1 allelic diversity has been widely reported in pea [14,21,25,26,27,38,40,41,42,43,44,51].…”

Section: Discussionmentioning

confidence: 99%

“…At the genomic level, seven alleles ( er1 -1/ er1mut1 , er1 -3, er1 -4, er1 -5, er1 -6, er1 -9, and er1 -10/ er1mut2 ) are the result of point mutations in the exons of wild-type PsMLO1 . Four alleles result from single base substitutions in wild-type PsMLO1 cDNA: in er1 -1, a C→G at position 680 (exon 6); in er1 -5, a G→A at position 570 (exon 5); in er1 -6, a T→C at position 1121 (exon 11); and in e r1 -10, a G→A at position 939 (exon 10) (Figure S3) [14,27,38,40]. Three alleles result from single base deletions in wild-type PsMLO1 cDNA, including ΔG at position 862 (exon 8) in er1 -3; ΔA at position 91 (exon 1) in er1 -4; and ΔT at position 928 (exon 10) in er1 -9 identified in this study [14] Two alleles result from small fragment deletions in wild-type PsMLO1 cDNA, including a 10-bp deletion of positions 111–120 (exon 1) in er1 -7 [26]; and a 3-bp deletion of positions 1339–1341 (exon 15) in er1 -8.…”

Section: Discussionmentioning

confidence: 99%

“…To date, nine er1 alleles resistant to E. pisi have been identified in E. pisi -resistant pea germplasms: er1 -1 (also known as er1mut1 ) [14,21,25,40,41], er1 -2 [14,24,25], er1 -3 [14], er1 -4 [14], er1 -5 [38], er1 -6 [27], er1 -7 [26], er1 -10 (also known as er1mut2 ) [21,40,42], and er1 -11 [42,43]. Each er1 allele corresponds to a different PsMLO1 mutation site and pattern.…”

Section: Introductionmentioning

confidence: 99%

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Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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Powdery mildew caused by Erysiphe pisi DC. severely affects pea crops worldwide. The use of resistant cultivars containing the er1 gene is the most effective way to control this disease. The objectives of this study were to reveal er1 alleles contained in 55 E. pisi-resistant pea germplasms and to develop the functional markers of novel alleles. Sequences of 10 homologous PsMLO1 cDNA clones from each germplasm accession were used to determine their er1 alleles. The frame shift mutations and various alternative splicing patterns were observed during transcription of the er1 gene. Two novel er1 alleles, er1-8 and er1-9, were discovered in the germplasm accessions G0004839 and G0004400, respectively, and four known er1 alleles were identified in 53 other accessions. One mutation in G0004839 was characterized by a 3-bp (GTG) deletion of the wild-type PsMLO1 cDNA, resulting in a missing valine at position 447 of the PsMLO1 protein sequence. Another mutation in G0004400 was caused by a 1-bp (T) deletion of the wild-type PsMLO1 cDNA sequence, resulting in a serine to leucine change of the PsMLO1 protein sequence. The er1-8 and er1-9 alleles were verified using resistance inheritance analysis and genetic mapping with respectively derived F2 and F2:3 populations. Finally, co-dominant functional markers specific to er1-8 and er1-9 were developed and validated in populations and pea germplasms. These results improve our understanding of E. pisi resistance in pea germplasms worldwide and provide powerful tools for marker-assisted selection in pea breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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“…OsMLO3 in rice (Devoto et al 2003), TaMLO_A1 and TaMLO_B1 in wheat (Devoto et al 2003;Várallyay et al 2012). But also in dicots MLO-like susceptibility genes have been discovered, such as AtMLO2, AtMLO6 and AtMLO12 in Arabidopsis (Consonni et al 2006), SlMLO1 in tomato (Bai et al 2008), PsMLO1 in pea (Humphry et al 2011;Pavan et al 2011;Santo et al 2013), CaMLO2 in pepper (Kim and Hwang 2012;Zheng et al 2013), LjMLO1 in lotus (Humphry et al 2011), and MtMLO1 in barrel clover (Humphry et al 2011). The MLO-genes have about seven transmembrane domains, and are located in the plasma membrane with an extracellular amino terminus and an intracellular carboxy terminus with a calmodulin binding domain (Kim et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

et al. 2014

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Powdery mildew (PM, caused by Podosphaera fusca) and downy mildew (DM, caused by Pseudoperonospora cubensis) are important diseases of cucumber (Cucumis sativus). Breeding for resistance has been undertaken since the 1940s, but underlying resistance genes have not been functionally analysed yet. The published genome sequence of cucumber catalyses the search for such genes. Genetic studies have indicated that resistances to PM and DM in cucumber are often inherited recessively, which indicates the presence of susceptibility genes (Sgenes). Therefore we analyzed the cucumber genome for homologs of functionally proven S-genes known from other plant species. We identified 13 MLO-like genes in cucumber, three of which cluster in Clade V, the clade that contains all known MLO-like susceptibility genes to powdery mildews in other dicots. The expression of one of these three genes, CsaMLO1, located on chromosome 1, was upregulated after PM inoculation. It co-localizes with a QTL for PM resistance previously identified. Also homologs of the susceptibility genes PMR4 and PMR5 are located at this QTL. The second MLO-like gene from Clade V (CsaMLO8) resides in a recessively inherited major QTL for PM resistance at the bottom of chromosome 5, together with a PMR6-like gene. Two major QTL for DM recessive resistance at the top of chromosome 5 co-localize with CsaDMR6-2, which is homologous to the DMR6 susceptibility gene in Arabidopsis. This study has identified several candidate genes for susceptibility to PM and DM in cucumber that may explain QTL for recessively inherited resistance, reported earlier.

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“…Looking at all the mlo-mutant alleles in Table 1, the highest number is found in barley (33), followed by wheat (16), petunia (2), pea (3) and tomato (1) [64][65][66][67], [68] and chapter 7].…”

Section: A)…through Natural Variationmentioning

confidence: 99%

Susceptibility pays off: insights into the mlo-based powdery mildew resistance

Appiano¹

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The ENU-induced powdery mildew resistant mutant pea (Pisum sativum L.) lines S(er1mut1) and F(er1mut2) harbour early stop codons in the PsMLO1 gene

Cited by 21 publications

References 14 publications

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

Susceptibility pays off: insights into the mlo-based powdery mildew resistance

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