The enzymatic determination of lactic acid in faeces in glycosidase deficiency

Clarke, Andrew D.; Podmore, D.A.

doi:10.1016/0009-8981(66)90138-0

Cited by 8 publications

(2 citation statements)

References 8 publications

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“…Since the fall in pH is the result of the formation of large quantities of low molecular weight organic acids, the estimation of lactic acid has been recommended as a useful test. Lactic acid can be determined by enzymatic or chemical methods; a simple technique has been developed by Clarke and Podmore (1966).…”

Section: Sugar Transportmentioning

confidence: 99%

Sugar malabsorption due to deficiencies of disaccharidase activities and of monosaccharide transport.

Holzel¹

1967

Archives of Disease in Childhood

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In many societies including our own, carbohydrates play a major part in the total calorie supply. According to the National Food survey figures (Greaves and Hollingsworth, 1964), the average daily diet of adults contains nearly 350 g. of this constituent. Diets of course vary with eating habits and social class, but the dietary carbohydrates are largely ingested as poly-, oligo-, and disaccharides. In the very young, however, the carbohydrate intake may consist almost entirely of disaccharides, though this phase is now getting progressively shorter. As the rapid decrease in breast-feeding has been followed by the early introduction of cereals into the cow's milk formulae, even the infant of only a few weeks of age has also to digest polysaccharides. Sjovall, 1957; Dahlqvist and Borgstrom, 1961) demonstrated that disaccharidase activity in the intestinal lumen was extremely limited and did not account for the amount of carbohydrate absorbed. They further indicated that the majority of disaccharides were absorbed unhydrolysed and split intracellularly, a fact that accorded well with experimental findings (Cajori, 1933;Fridhandler and Quastel, 1955).The problem of localization of the sugar-splitting enzymes was brought nearer its solution by Miller and Crane (1961), who were able to separate the brush-border from the rest of the intestinal mucosa of the hamster, and found that the whole of the enzyme activity of the mucosa was accounted for by this layer. (-galactosidase (lactase) activity had been localized in the microsomes of the rat intestinal mucosa by Doell and Kretchmer (1962), while Dahlqvist and Brun (1962), employing histochemical methods for the recognition of invertase and trehalase in various animal tissues, associated their activity with cytoplasmic granules. As yet there is no definite information concerning the possible relationship of the elements of fine structure of the microvillus with its absorptive and digestive functions. However, two related fractions have been obtained by density gradient centrifugation of tris-disrupted brush-borders from hamster intestinal mucosa, and have been identified as the microvillus cores and their surrounding membranous coats (Overton, Eichholz, and Crane, 1965;Eichholz and Crane, 1965).

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Section: Sugar Transportmentioning

confidence: 99%

Sugar malabsorption due to deficiencies of disaccharidase activities and of monosaccharide transport.

Holzel¹

1967

Archives of Disease in Childhood

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“…The findings in babies who had had surgery for large bowel disease suggest that symptoms are the result of disaccharidase deficiency. A single case of lactose intolerance which developed in an infant with a colostomy fashioned because of imperforate anus has been reported (Clarke and Podmore, 1966).…”

Section: Discussionmentioning

confidence: 99%

Sugar Intolerance as a Cause of Protracted Diarrhoea Following Surgery of the Gastrointestinal Tract in Neonates

Burke

Anderson

1966

J Paediatr Child Health

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EDITORIAL SYNOPSIS Osmotic diarrhoea, resulting from disturbed sugar digestion and absorption is shown to occur quite frequently following a variety of gastrointestinal surgical procedures in neonates. These include gastrostomy, small bowel anastomosis and colostomy. Suitable manipulation of the sugar content of the diet will mitigate the symptoms, thus averting fluid loss, decreasing morbidity and sometimes mortality.

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Die intestinale Hydrolyse der Kohlenhydrate beim Säugling

Auricchio¹,

Pietra²

1968

Fortschritte Der Pädologie

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The enzymatic determination of lactic acid in faeces in glycosidase deficiency

Cited by 8 publications

References 8 publications

Sugar malabsorption due to deficiencies of disaccharidase activities and of monosaccharide transport.

Sugar malabsorption due to deficiencies of disaccharidase activities and of monosaccharide transport.

Sugar Intolerance as a Cause of Protracted Diarrhoea Following Surgery of the Gastrointestinal Tract in Neonates

Die intestinale Hydrolyse der Kohlenhydrate beim Säugling

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