The Epidemiology Research of Blastocystis hominis in the Dokuz Eylül University Medical Faculty Hospital between 2005 and 2009

İnceboz, Tonay; Usluca, Selma; Över, Leyla; Yalçin, Gülter; Tuncay, Sema; Özkoç, Soykan

doi:10.5152/tpd.2011.19

Cited by 11 publications

(6 citation statements)

References 5 publications

(8 reference statements)

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“…Although it has been reported that maleness creates a statistically significant difference in the Blastocystis prevalence [29], there have also been studies demonstrating that there was a higher prevalence in females [30], or that there was no difference between the genders [25,26]. Similar to the study by Ertug et al [29], we identified a statistically significant relationship between the male gender and the Blastocystis-positivity (p = 0.020).…”

Section: Hemodialysis Patients P Valuesupporting

confidence: 82%

Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey

Gülhan

Aydın

Demirkazik

et al. 2020

J Infect Dev Ctries

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Introduction: The aim of this study was to determine the Blastocystis prevalence and subtypes in hemodialysis patients in Turkey. Methodology: Eighty-four patients diagnosed with end-stage renal failure who were undergoing hemodialysis and 20 healthy volunteers were enrolled. Blastocystis presence was investigated by native-Lugol, trichrome staining, PCR using STS primers, and DNA sequencing analysis. Results: Among the stool samples from the hemodialysis patients, 9.52% (8/84) were found to be Blastocystis-positive with the native-Lugol and trichrome staining. Seven of the eight Blastocystis isolates were subtyped using STS primers. Blastocystis subtype distribution was as follows: one had ST1, two had ST2, two had ST3, two had ST3+ST6, and one was not subtyped. Blastocystis positivity was detected in two healthy control (2/20, %10), one subject had ST1, and the other was not subtyped. All subtypes identified by PCR were confirmed by the sequencing analysis. In the two samples that had mixed subtypes (ST3+ST6) when using the STS primers, only ST3 was detected in the sequencing analysis. Although some patients have multiple symptoms, the most common symptoms in Blastocystis positive patients were bloating (5/8), diarrhea (4/8), nausea and vomiting (2/8), and gas and weight loss (1/8). Also, only one patient had Giardia intestinalis. Conclusions: This was the first study to determine the Blastocystis subtypes in hemodialysis patients. A rare subtype, ST6, was identified in two of the patients. Thus, the ST6 infections were attributable to transmission from poultry infections. The presence of this unusual subtype suggests the need for further extensive studies of hemodialysis patients.

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Section: Hemodialysis Patients P Valuesupporting

confidence: 82%

Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey

Gülhan

Aydın

Demirkazik

et al. 2020

J Infect Dev Ctries

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“…Previous studies that were examined using different parasitological detection methods (Yaicharoen et al, 2005) or conducted in rural areas and in schools revealed a high prevalence of B. hominis (Warunee et al, 2007;Kitvatanachai et al, 2008). Other hospital-based studies may have shown a higher prevalence of blastocystosis compared to this study because those studies were conducted on patients with GI symptoms (Rostami Nejad et al, 2010;Gonzalez-Moreno et al, 2011;Inceboz et al, 2011;Paschke et al, 2011). FECT, as used for other protozoa and fecal parasites, generally appears to be unsuitable for B. hominis because it causes disruption of the organism (Stenzel and Boreham, 1996).…”

Section: Discussionmentioning

confidence: 62%

A Hospital-Based Study of Epidemiological and Clinical Data onBlastocystis hominisInfection

Laodim

Intapan

Sawanyawisuth

et al. 2012

Foodborne Pathogens and Disease

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Blastocystis hominis is a foodborne protozoan found in the human feces worldwide. One hundred and ninety-nine individuals with stool samples positive for B. hominis were identified from a pool of 14,325 patient stools collected between 2003 and 2010 from Srinagarind hospital in Thailand. The medical records of patients were reviewed for demographic and clinical data. Of the 85 patients (42.7%) who had B. hominis infection with no co-infections, 42.5% experienced gastrointestinal symptoms. Abdominal pain is the most frequently observed symptom followed by diarrhea. Strongyloides stercolaris and Opisthorchis viverrini were the predominant parasitic co-infections in blastocystosis patients. The infection rates of B. hominis were high during the rainy season. Most B. hominis-infected patients (94%) had underlying diseases; malignancy and chronic diseases were equally top ranked (35.3%) which indicated that B. hominis is an opportunistic protozoan.

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“…due to parasite form was determined as follow: Vacuolar form in 525 (67.49%), granular in 115 (14.78%), both vacuolar and granular in 138 (17.73%) cases. [ 31 ]…”

Section: Discussionmentioning

confidence: 99%

Laboratory diagnosis of Blastocystis spp. in diarrheic patients

El-Ghareeb¹,

Younis²,

Fakahany³

et al. 2015

Trop Parasitol

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Background:Many laboratories currently diagnose Blastocystis spp. infections by looking for the presence of vacuolar forms in faeces and the amoeboid form in diarrheal stools.Objectives:To investigate the best direct method in diagnosis of Blastocystis spp. and to study different morphological forms of the parasite.Materials and Methods:The study was carried out on one thousand and two hundred diarrheic stool samples. All samples were examined using direct smear, iodine stained smear, formalin-ether concentration techniques, trichrome stained smear and in vitro cultivation using Jones' medium.Results:Using direct smear, Blastocystis spp was detected in 42 cases (3.5%) with a sensitivity (28.4%) and specificity (100%). Iodine stained smear detected 72 positive cases (6%) with a sensitivity (48.7%), specificity (100%). Formol ether concentration technique detected 120 positive cases (10%) with a sensitivity (81.1%) and specificity (100%). Trichrome stained smear detected 148 positive cases (12.3%). In vitro cultivation using Joni's medium detected 274 positive cases (22.8%) which was the highest number among all different diagnostic methods with a sensitivity (100%) ,specificity (88%), PPV (54.1%) and NPV (100%). It was found that, 49 blastocystosis cases had mixed infection with other intestinal parasites. Giardia lamblia was the most frequently associated parasite with Blastocystis spp.Conclusion:In vitro cultivation is more sensitive in detection of B. hominis than simple smear and concentration technique. Blastocystis spp. vacuolar form was the most common form that was found by all methods used in this study G. lamblia was the most frequent parasite associated with Blastocystis spp .

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The Epidemiology Research of Blastocystis hominis in the Dokuz Eylül University Medical Faculty Hospital between 2005 and 2009

Abstract: As B. hominis was the most frequently seen parasite in patients with gastrointestinal complaints, we suggest that the parasite should be considered as pathogenic and sufficient attention must be paid in routine stool examinations.

Cited by 11 publications

References 5 publications

Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey

Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey

A Hospital-Based Study of Epidemiological and Clinical Data onBlastocystis hominisInfection

Laboratory diagnosis of Blastocystis spp. in diarrheic patients

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