The Epistemology of G. E. Moore.

Koehl, Richard; Klemke, E. D.

doi:10.2307/2105756

Cited by 1 publication

(2 citation statements)

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“…Till now, intravital optical imaging approaches still depend largely on surgery-established imaging windows for visualization of blood vessels and cells in situ [9,36]. However, the surgery itself might adversely produce a local, and sometimes even systemic proinflammatory stimulus [35], which may directly impair the behaviors of cells.…”

Section: Discussionmentioning

confidence: 99%

“…Skin, as the outermost organ of the body, is an inevitable barrier to perform intravital optical imaging, whose strong scattering characteristic limits the penetration of light into tissue. In order to resolve this problem, a dorsal skin chamber window based on surgical operation was developed, which has been widely used in tumor studies and pharmacokinetics [9][10][11], etc. It was well known that surgical operations were always accompanied with bleeding and changes of the normal physiological environment during process of model establishment [10,11].…”

Section: Introductionmentioning

confidence: 99%

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FSOCA‐induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo

Shi¹,

Feng²,

Zhang³

et al. 2017

Journal of Biophotonics

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The mouse footpad for its feature of hairlessness provides an available window for imaging vascular and cellular structure and function in vivo. Unfortunately, the strong scattering of its skin limits the penetration of light and reduces the imaging contrast and depth. Herein, an innovative footpad skin optical clearing agent (FSOCA) was developed to make the footpad skin transparent quickly by topical application. The results demonstrate that FSOCA treatment not only allowed the cutaneous blood vessels and blood flow distribution to be monitored by laser speckle contrast imaging technique with higher contrast, but also permitted the fluorescent cells to be imaged by laser scanning confocal microscopy with higher fluorescence signal intensity and larger imaging depth. In addition, the physiological saline-treatment could make the footpad skin recover to the initial turbid status, and reclearing would not induce any adverse effects on the distributions and morphologies of blood vessels and cells, which demonstrated a safe and switchable window for biomedical imaging. This switchable footpad skin optical clearing window will be significant for studying blood flow dynamics and cellular immune function in vivo in some vascular and immunological diseases.

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Section: Discussionmentioning

confidence: 99%