The Equilibria of Isomerase and Aldolase, and the Problem of the Phosphorylation of Glyceraldehyde Phosphate

Meyerhof, Otto; Junowicz-Kocholaty, R.

doi:10.1016/s0021-9258(18)72218-7

Cited by 92 publications

(14 citation statements)

References 10 publications

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“…In the light, chloroplast export of DHAP is favoured by the equilibrium position of stromal TPI, which is strongly on the side of DHAP (Walker, 1976; Knowles and Albery, 1977). Sharkey and Weise (2012) calculated there should be over 20 times more DHAP than GAP at equilibrium (Meyerhof and Junowicz-Kocholaty, 1943; Bassham and Krause, 1969). The substrate affinities of the triose phosphate translocator for DHAP and GAP are similar at K m 0.13 mM and K m =0.08 mM, respectively (Fliege et al ., 1978).…”

Section: Resultsmentioning

confidence: 99%

Intramolecular carbon isotope signals reflect metabolite allocation in plants

Wieloch

Sharkey

Ra³

et al. 2021

Preprint

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- Stable isotopes at natural abundance are key tools to study physiological processes occurring outside the temporal scope of manipulation and monitoring experiments. Whole-molecule carbon isotope ratios (13C/12C) enable assessments of plant carbon uptake yet conceal information about carbon allocation. Here, we identify an intramolecular 13C/12C signal at tree-ring glucose C-5 and C-6, develop experimentally testable theories on its origin, and test these theories. - First, we assess the potential of processes within C3 metabolism for signal introduction based (inter alia) on constraints on signal propagation posed by metabolic networks. Second, we support our theoretical framework by re-analysing published 13C/12C data and modelling signal-environment relationships. - We propose the intramolecular signal reports carbon allocation into major metabolic pathways in actively photosynthesising leaf cells including the anaplerotic, shikimate, and non-mevalonate pathway. This is supported by previously reported whole-molecule 13C/12C increases in cellulose of ozone-treated Betula pendula and a highly significant relationship between the intramolecular signal and tropospheric ozone concentration. Additionally, we postulate a pronounced preference of leaf-cytosolic triose-phosphate isomerase to catalyse the forward reaction in vivo (dihydroxyacetone phosphate to glyceraldehyde 3-phosphate). - In conclusion, intramolecular 13C/12C analysis resolves information about carbon uptake and allocation enabling more comprehensive assessments of carbon metabolism than whole-molecule 13C/12C analysis.

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Section: Resultsmentioning

confidence: 99%

Intramolecular carbon isotope signals reflect metabolite allocation in plants

Wieloch

Sharkey

Ra³

et al. 2021

Preprint

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“…Also, in trióse phosphate the equilibrium favors the keto form by a 20:1 ratio. 21,22 Ease of deprotonation can be predicted for the steroid side chain.…”

Section: A-°hmentioning

confidence: 99%

The Solvolysis of 21-Hydrocortisone Esters and Hemiesters

Garrett¹

1962

J. Med. Chem.

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The 21-hemiester of hydrocortisone and succinic acid is hydrolyzed at faster rates in the neutral pH region than would be predicted from studies on specific hydrogen and hydroxyl ion catalysis. This can be attributed to either intramolecular catalyzed hydrolysis by the terminal carboxylate anion or by an enhanced rate of specific hydroxyl ion attack on the undissociated hemiester.No highly significant difference in hydrolysis of the hemiester anion by specific hydroxyl ion catalysis under comparable conditions was observed for increased length of the dicarboxylic acid, from the hemisuccinate to the hemisuberate. Steric blocking by substituents on the a or ß carbons of the carboxyalkyl group is more effective in modification of the alkaline hydrolysis rate than anion distance from the ester group for the hemiesters studied, ROOC(CH2)reCOO",

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“…In the light, chloroplast export of DHAP is favoured by the equilibrium position of stromal TPI, which is strongly on the side of DHAP ( Walker, 1976 ; Knowles and Albery, 1977 ). Sharkey and Weise (2012) calculated that there should be >20 times more DHAP than GAP at equilibrium ( Meyerhof and Junowicz-Kocholaty, 1943 ; Bassham and Krause, 1969 ). The substrate affinities of the triose phosphate translocator for DHAP and GAP are similar at K m =0.13 mM and K m =0.08 mM, respectively ( Fliege et al , 1978 ).…”

Section: Resultsmentioning

confidence: 99%

Intramolecular carbon isotope signals reflect metabolite allocation in plants

Wieloch

Sharkey

Werner

et al. 2022

Journal of Experimental Botany

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Stable isotopes at natural abundance are key tools to study physiological processes occurring outside the temporal scope of manipulation and monitoring experiments. Whole-molecule carbon isotope ratios ( 13C/ 12C) enable assessments of plant carbon uptake yet conceal information about carbon allocation. Here, we identify an intramolecular 13C/ 12C signal at tree-ring glucose C-5 and C-6 and develop experimentally testable theories on its origin. More specifically, we assess the potential of processes within C3 metabolism for signal introduction based (inter alia) on constraints on signal propagation posed by metabolic networks. We propose that the intramolecular signal reports carbon allocation into major metabolic pathways in actively photosynthesising leaf cells including the anaplerotic, shikimate, and non-mevalonate pathway. We support our theoretical framework by linking it to previously reported whole-molecule 13C/ 12C increases in cellulose of ozone-treated Betula pendula and a highly significant relationship between the intramolecular signal and tropospheric ozone concentration. Our theory postulates a pronounced preference of leaf-cytosolic triose-phosphate isomerase to catalyse the forward reaction in vivo (dihydroxyacetone phosphate to glyceraldehyde 3-phosphate). In conclusion, intramolecular 13C/ 12C analysis resolves information about carbon uptake and allocation enabling more comprehensive assessments of carbon metabolism than whole-molecule 13C/ 12C analysis.

show abstract

The Equilibria of Isomerase and Aldolase, and the Problem of the Phosphorylation of Glyceraldehyde Phosphate

Cited by 92 publications

References 10 publications

Intramolecular carbon isotope signals reflect metabolite allocation in plants

Intramolecular carbon isotope signals reflect metabolite allocation in plants

The Solvolysis of 21-Hydrocortisone Esters and Hemiesters

Intramolecular carbon isotope signals reflect metabolite allocation in plants

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