The ERF‐VII transcription factor SmERF73 coordinately regulates tanshinone biosynthesis in response to stress elicitors in Salvia miltiorrhiza

Abstract

Summary Here, we investigate the role of SmERF73, a group VII ETHYLENE RESPONSE FACTOR stress response transcription factor, in the regulation of post‐modification of the skeleton precursors of diterpene tanshinones in Salvia miltiorrhiza. Most genes found to be involved in tanshinone biosynthesis are located on chromosome 6, and five of these genes comprise a large gene cluster in S. miltiorrhiza. We found that SmERF73 overexpression in S. miltiorrhiza coordinately up‐regulated the transcription of seven tanshinone biosynthetic genes, four of which were located in the tanshinone gene cluster, consequently increasing tanshinone accumulation, while SmERF73 silencing reduced corresponding gene transcription and tanshinone accumulation. SmERF73 recognizes GCC‐box promoter elements of four tanshinone‐associated genes (DXR1, CPS1, KSL1 and CYP76AH3) and activates their expression. Moreover, SmERF73 and its targets were up‐regulated by stress elicitors; SmERF73 appears to be at least partly mediated by the jasmonic acid (JA) signaling pathway via interaction with SmJAZ3. SmERF73 coordinately regulates tanshinone biosynthetic gene expression, suggesting a potential link between tanshinone production and plant stress responses.

Increasing Expression of PnGAP and PnEXPA4 Provides Insights Into the Enlargement of Panax notoginseng Root Size From Qing Dynasty to Cultivation Era

“…Y1H assay was performed as described previously (Zheng et al, 2021 ). The sequences of proPnEXPA4, proPnGGPPS3, proPnFPS, proPnCYP716A47, proPnCYP716A53v2 , and proPnGAP were nested PCR-amplified according to genomic sequences (Jiang et al, 2020 ).…”

“…EMSA was performed as described previously (Zheng et al, 2021 ). The full-length cDNA of PnPHL8 was first cloned into a pMAL-c2x vector and then transformed into a Rosetta (DE3) competent cell.…”

Comparative Transcriptomics and Metabolites Analysis of Two Closely Related Euphorbia Species Reveal Environmental Adaptation Mechanism and Active Ingredients Difference

“…To provide a preliminary indication of genetic variation within species, the high-quality mRNA from leaves, stems, and roots from three individual plants for each species was isolated using Plant RNA Purification Reagent (Invitrogen, Life Technologies, USA) following the manufacturer's protocol. Both RNA-seq library preparation and paired-end sequencing were performed using an Illumina HiSeq 6000 platform (Zheng et al, 2021 ). All unigenes were first subjected to BLASTX (Altschul et al, 1997 ) against the National Center for Biotechnology Information (NCBI), including the non-redundant protein (Nr) database and non-redundant nucleotide sequence (Nt) databases with an E -value threshold of 10 −5 .…”

Data_Sheet_1.ZIP

“…Y1H assay was performed as described previously (Zheng et al, 2021). The sequences of proPnEXPA4, proPnGGPPS3, proPnFPS, proPnCYP716A47, proPnCYP716A53v2, and proPnGAP were nested PCR-amplified according to genomic sequences (Jiang et al, 2020).…”

“…EMSA was performed as described previously (Zheng et al, 2021). The full-length cDNA of PnPHL8 was first cloned into a pMAL-c2x vector and then transformed into a Rosetta (DE3) competent cell.…”