The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

Wang, Haihai; Guo, Ying; Lv, Fenni; Zhu, Huayu; Wu, Shengyuan; Jiang, Yanjie; Li, Feifei; Zhou, Baoliang; Guo, Wangzhen; Zhang, Tianzhen

doi:10.1007/s11103-009-9578-7

Cited by 72 publications

(62 citation statements)

References 41 publications

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“…In cotton fibers and trichomes, there exists a positive correlation between pectic sheath and elongation [79] and recent studies by [80] have established that pectic polysaccharides and xyloglucan containing uronic acids were the major polysaccharides extracted during elongation. Hence, our results are in agreement with various studies which state that pectin biosynthesis promotes fiber elongation [81] and that the degree of esterification is a key factor in controlling the elongation [38], [82]. The correlation between length and HG was not detected in the sPLS analysis most likely because the stronger (negative) correlation of HG with micronaire.…”

Section: Discussionsupporting

confidence: 92%

Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides

et al. 2014

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A detailed knowledge of cell wall heterogeneity and complexity is crucial for understanding plant growth and development. One key challenge is to establish links between polysaccharide-rich cell walls and their phenotypic characteristics. It is of particular interest for some plant material, like cotton fibers, which are of both biological and industrial importance. To this end, we attempted to study cotton fiber characteristics together with glycan arrays using regression based approaches. Taking advantage of the comprehensive microarray polymer profiling technique (CoMPP), 32 cotton lines from different cotton species were studied. The glycan array was generated by sequential extraction of cell wall polysaccharides from mature cotton fibers and screening samples against eleven extensively characterized cell wall probes. Also, phenotypic characteristics of cotton fibers such as length, strength, elongation and micronaire were measured. The relationship between the two datasets was established in an integrative manner using linear regression methods. In the conducted analysis, we demonstrated the usefulness of regression based approaches in establishing a relationship between glycan measurements and phenotypic traits. In addition, the analysis also identified specific polysaccharides which may play a major role during fiber development for the final fiber characteristics. Three different regression methods identified a negative correlation between micronaire and the xyloglucan and homogalacturonan probes. Moreover, homogalacturonan and callose were shown to be significant predictors for fiber length. The role of these polysaccharides was already pointed out in previous cell wall elongation studies. Additional relationships were predicted for fiber strength and elongation which will need further experimental validation.

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Section: Discussionsupporting

confidence: 92%

Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides

et al. 2014

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“…For pathogen defense, the accumulation of pectin increases hydrogen bonding in the extracellular matrix, resulting in decreased nutrient availability to the pathogen (Vogel et al, 2002). The decrease of de-esterified pectin in cotton fiber can promote its elongation (Wang et al, 2010). Our study also demonstrates that the mutant phenotype of del1 depends on the alteration of cell wall composition and structure.…”

Section: Possible Molecular Mechanism Of Del1 Responsible For the Mutmentioning

confidence: 58%

“…PELs have been extensively studied in plant pathogenic bacteria such as Erwinia chrysanthemi, which causes soft-rot diseases (Barras et al, 1994). In plants, multiple functions have been identified for PEL, and there is some suggestion that it is involved in pollen, anthers, pistils, and developing tracheary elements (Wing et al, 1990;Rogers et al, 1992;Wu et al, 1996;Kulikauskas and McCormick, 1997;Domingo et al, 1998;Milioni et al, 2001), fruit softening and ripening (Dominguez-Puigjaner et al, 1997;Medina-Escobar et al, 1997;Nunan et al, 2001;Pua et al, 2001), lateral root emergence (Laskowski et al, 2006), cotton fiber elongation (Wang et al, 2010), leaf senescence (Wu et al, 2013), and susceptibility to plant pathogens (Vogel et al, 2002). It has also been shown to be expressed in a wide range of tissues (Palusa et al, 2007;Sun and van Nocker, 2010).…”

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confidence: 99%

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function-and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

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“…Transgenic modification of cell wall structural proteins and enzymes has also been carried out. Li et al (2009) reported that suppression of fasciclin-like arabinogalactan proteins by expression of a GhABP4-targeted RNAi transgene inhibited fiber elongation resulting in shorter mature fibers, while antisense suppression of the pectate lyase gene GhPEL also reduced fiber elongation (Wang et al 2010a).…”

Section: Discussionmentioning

confidence: 99%

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

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et al. 2010

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Plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases (XTH) that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. In Arabidopsis and other plants, XTHs are encoded by relatively large gene families that are regulated in specific spatial and temporal patterns. In silico screening of a cotton expressed sequence tag (EST) database identified 23 sequences with close sequence similarity to Arabidopsis XTH coding sequences. Analysis of full-length cotton cDNAs derived from these ESTs allow for the identification of three distinct GhXTH cDNAs (denoted GhXTH1, GhXTH2 and GhXTH3) based primarily on their 3' untranslated sequences. The three GhXTH genes were expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers. The function of GhXTH1 in mediating cotton fiber elongation was analyzed in transgenic cotton plants that express a transgene consisting of the GhXTH1 coding sequence under transcriptional control of the CaMV 35S promoter. Plants that over-expressed GhXTH1 had increased XTH activity and produced mature cotton fibers that were between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions. Segregation analysis showed that the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton. These results confirm that GhXTH1 is the predominant XTH in elongating fibers and its expression limits cotton fiber elongation.

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The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

Cited by 72 publications

References 41 publications

Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides

Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

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