The establishment of a highly sensitive ELISA for detecting bovine serum albumin (BSA) based on a specific pair of monoclonal antibodies (mAb) and its application in vaccine quality control

Zhang, Kui; Song, Chaojun; Li, Qi; Li, Yongming; Yang, Kun; Jin, Boquan

doi:10.4161/hv.6.8.11935

Cited by 29 publications

(16 citation statements)

References 15 publications

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“…This proposed method can be efficiently used to detect albumin as a standard biomarker for detecting diseases associated with hypoalbuminemia (<30 mg mL −1 ), such as liver diseases, gastrointestinal protein loss, edema and hyperalbuminemia (>30 mg mL −1 ), such as severe dehydration and abnormal increase in body fat. 43,44 The accuracy of the proposed method is comparable to that of the most commonly used method for detecting albumin from biological fluids, the enzyme linked immunosorbent assay (ELISA), 45,46 which is sensitive and selective but is very time consuming and requires extensive sample preparation steps.…”

Section: Discussionmentioning

confidence: 90%

Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing

Parachalil

Brankin

McIntyre

et al. 2018

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Section: Discussionmentioning

confidence: 90%

Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing

Parachalil

Brankin

McIntyre

et al. 2018

Analyst

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“…FMU-BSA 6 as the capture antibody and FMU-BSA 11 as the detection antibody were produced from hybridoma cell lines of secreting monoclonal antibody against BSA by ourselves [31]. PHPPA was from Acros Organics (USA), and TCPO was supplied by Tokyo Kasei Kodgyo (Japan).…”

Section: Reagents and Solutionsmentioning

confidence: 99%

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Xue

Zhang

et al. 2012

Appl Biochem Biotechnol

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Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vaccines. A typical "sandwich type" immunoassay was used. Reaction of 3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea, catalyzed by HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimer, which was detected by chemiluminescence analysis with the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H(2)O(2)-glyoxaline-PHPPA dimer chemiluminescent system. This method exhibited high performance with a linear correlation between response and amount of bovine serum albumin (BSA) in the range 0.1 to 100.0 ng mL(-1) (r = 0.9988), and the detection limit was 0.03 ng mL(-1) (S/N = 3). Intra- and interassay coefficient variations were all lower than 9.0% at three concentrations (1.0, 20.0, and 80.0 ng mL(-1)). The proposed method has been used for successful analysis of the amount of residual BSA in vaccines. The results obtained compared well with those obtained by conventional colorimetric ELISA and luminol chemiluminescent ELISA.

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“…In addition, the device selectively responded to albumin (Figure c). The obtained sensitivity in our fabricated device was higher than those of the previous reports using fluorescent probes and immunoassays for albumin . This increased sensitivity could be attributed to the following reasons: 1) A long hydrophobic alkyl chain can improve the accessibility of the Ni II ‐nta terminal to the histidine residues in albumin.…”

Section: More Effective Materials For Ofet‐based Protein Assays: Coormentioning

confidence: 65%

Protein Assays on Organic Electronics: Rational Device and Material Designs for Organic Transistor‐Based Sensors

et al. 2020

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Artificial receptor-based protein assays have various attractive features such as a long-term stability, a low-cost production process, and the ease of tuning the target specificity. However, such protein sensors are still immature compared with conventional immunoassays. To enhance the application potential of synthetic sensing materials, organic field-effect transistors (OFETs) are some of the suitable platforms for protein assays because of their solution processability, durability, and compact integration. Importantly, OFETs enable the electrical readout of the protein recognition phenomena of artificial receptors on sensing electrodes. Thus, we believe that OFETs functionalized with artificial protein receptors will be a powerful tool for the on-site analyses of target proteins. In this Minireview, we summarize the recent progress of the OFET-based protein assays including the rational design strategies for devices and sensing materials.[a] Dr.

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The establishment of a highly sensitive ELISA for detecting bovine serum albumin (BSA) based on a specific pair of monoclonal antibodies (mAb) and its application in vaccine quality control

Abstract: The establishment of a highly sensitive ELISA for detecting bovine serum albumin (BSA) based on a specific pair of monoclonal antibodies (mAb) and its application in vaccine quality control, Human Vaccines, 6:8,[652][653][654][655][656][657][658]

Cited by 29 publications

References 15 publications

Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing

Raman spectroscopic analysis of high molecular weight proteins in solution – considerations for sample analysis and data pre-processing

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Protein Assays on Organic Electronics: Rational Device and Material Designs for Organic Transistor‐Based Sensors

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