The establishment of metrological characteristics of the method “Ethanol as Internal Standard” for the direct determination of volatile compounds in alcoholic products

Charapitsa, Siarhei; Sytova, S.; Korban, Anton; Boyarin, Nicolai; Shestakovich, Inna; Čabala, Radomír

doi:10.25135/jcm.14.18.02.063

Cited by 11 publications

(11 citation statements)

References 12 publications

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“…SS‐2 and SS‐3 as the most diluted samples exhibited slightly worse results. We already observed this phenomenon during our previous works with FID . The reason for the worse biases could consist in chemical transformations of the reactive acetaldehyde and esters or in the evaporation.…”

Section: Resultsmentioning

confidence: 69%

“…We already observed this phenomenon during our previous works with FID. 10,11 The reason for the worse biases could consist in chemical transformations of the reactive acetaldehyde and esters or in the evaporation. Nevertheless, the results are still acceptable, and the described method can be undoubtedly regarded as true.…”

Section: Resultsmentioning

confidence: 99%

“…This paper is a further development of our previously published method, which employs ethanol as an IS during direct determination of volatile compounds in ethanol‐containing products with GC‐FID . This method was first described in the publications, where it was shown that employing ethanol as an IS creates great perspectives for this method in analysis of alcoholic beverages and products of their origin.…”

Section: Introductionmentioning

confidence: 99%

“…Although its peak area was more than 100 000 times greater than that of the analysed volatiles, ethanol was successfully employed in direct quantification. [9][10][11][12][13][14] A specific procedure should be employed for the MS. Here, we had to tune the ethanol peak area within the linear dynamic range of the MS detector.…”

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confidence: 99%

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The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

et al. 2020

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The potential use of ethanol as an internal standard (IS) for GC‐MS analysis was studied. The paper describes the analysis of spirit drinks and other alcoholic products which consist of a mixture of water, ethanol, and volatile compounds. In the suggested method, ethanol was employed as an IS for the common procedure of volatile compounds quantification. A number of standard solutions of nine compounds with different concentrations was prepared in a water‐ethanol matrix and measured with GC‐MS in the SIM mode. Two possible approaches were suggested to avoid detector saturation during ethanol detection. The first one consisted in using less abundant m/z 47 as quantifiers. These ions mainly correspond to unfragmented heavy ethanol molecules containing one 13C isotope. The second method consisted in reduction of the voltage of MS electron multiplier. The experiment also included the preparation and subsequent dilution of the standard solution and ethanol with water, which determined the linearity of the modified MS response relative to the ethanol content. Analysis of the obtained results revealed that volatile compounds can be successfully accurately determined with GC‐MS by employing ethanol as an IS. Application of the suggested method is not limited to the reported volatile compounds and alcoholic products.

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Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

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“…This paper is a logical continuation of a single-laboratory validation of the "Ethanol as Internal Standard" method [1]. The latter was already proved to be accurate and precise during single-laboratory experiment [2] and interlaboratory approbation within 3 laboratories [3]. The discussed method is applied for gas chromatographic (GC) direct quantification of volatile compounds in spirit drinks and other ethanol containing samples.…”

Section: Introductionmentioning

confidence: 99%

Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products

et al. 2019

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A collaborative interlaboratory study on the method of direct quantitation of volatile compounds in spirit drinks and alcoholic products was conducted. The discussed method applies ethanol, the major volatile component of an alcoholic product, as an internal standard. In this study 9 laboratories from 4 different countries were supplied with standard solutions for gas chromatographic measurements. Five aqueous ethanol 40% (v/v) standard solutions containing target compounds in concentrations ranging from 10 mg/L to 400 mg/L of absolute alcohol were prepared and sent to the participants for quantification of acetaldehyde, methyl acetate, ethyl acetate, methanol, 2-propanol, 1-propanol, 2-methyl-1-propanol, 1-butanol and 3-methyl-1-butanol. The interlaboratory study was evaluated according to the ISO 5725 standards and the Eurachem guide. The within-laboratory precision varied between 0.4% and 7.5% for all samples and compounds, showing a sufficiently high repeatability of the method. The between-laboratory precision was found to vary within a satisfactory range of 0.5% ÷ 10.0%. Precision of the method was well within the range predicted by the Horwitz equation for all analytes. The analysis of trueness showed that the bias of the method is insignificant at the significance level α = 5%. The determined concentrations of the analytes compared well to the gravimetrical values thus showing very satisfactory accuracy of the method. The results of the interlaboratory study confirmed that “Ethanol as Internal Standard” method is robust and reliable and can be used as a standard reference method for analysing volatile compounds in water-ethanol samples. The possibilities of method validation according to the previously obtained experimental data were shown.

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The Method for Direct Gas Chromatographic Determination of Acetaldehyde, Methanol, and Other Volatiles Using Ethanol as a Reference Substance: Application for a Wide Range of Alcoholic Beverages

et al. 2021

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The article presents the results of collaborative study of the method for gas chromatographic determination of alcoholic fermentation volatile by-products (acetaldehyde, methanol, methyl acetate, ethyl acetate, propan-2-ol, propan-1-ol, 2-methylpropan-1-ol, butan-1-ol, and 3-methylbutan-1-ol) in alcoholic beverages with volume ethanol content in a range of 9.9-96.0% (wine, sake, liquor, tequila, vodka, grappa, bourbon, scotch, calvados, rakia, rum, brandy, whiskey, gin, and rectified ethyl alcohol). The zest of the method is the use of ethanol, present in beverages, as a reference substance for quantitative determination of mentioned components. Checking of precision data, obtained under repeatability conditions and under both repeatability and intermediate precision conditions, and trueness of test results, was carried out according to the ISO 5725-6. The relative difference between calibration coefficients (relative response factors for analyzed volatile compound relative to ethanol), obtained with an interval of 3 weeks, did not exceed 1.1%. Contents of acetaldehyde, methanol, and volatiles in 15 commercial alcoholic beverages analyzed by the developed method varied in the 1.84-677 mg L −1 of absolute ethanol, 3.25-12,394 mg L −1 of absolute ethanol, and 1.49-4243 mg L −1 of absolute ethanol intervals, correspondingly.

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The establishment of metrological characteristics of the method “Ethanol as Internal Standard” for the direct determination of volatile compounds in alcoholic products

Cited by 11 publications

References 12 publications

The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products

The Method for Direct Gas Chromatographic Determination of Acetaldehyde, Methanol, and Other Volatiles Using Ethanol as a Reference Substance: Application for a Wide Range of Alcoholic Beverages

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