The establishment of the species-delimits and varietal-identities of the cultivated germplasm of Luffa acutangula and Luffa aegyptiaca in Sri Lanka using morphometric, organoleptic and phylogenetic approaches

Kumari, Sweety; Nakandala, N. D. U. S.; Nawanjana, P. W. I.; Rathnayake, R. M. S. K.; Senavirathna, H. M. T. N.; Senevirathna, R. W. K. M.; Wijesundara, W. M. D. A.; Ranaweera, L. T.; Mannanayake, M. A. D. K.; Weebadde, Cholani

doi:10.1371/journal.pone.0215176

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…Luffa aegyptiaca (Mill) is a member of the Cucurbitaceae family (2). There are about nine species in the genus Luffa including Luffa acutangula, Luffa cylindrica, Luffa aegyptiaca, Luffa operculata, L. graveolens and L. echinata (3). Luffa cylindrica (found mostly in South America) is the most widely published and cultivated (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…Luffa cylindrica (found mostly in South America) is the most widely published and cultivated (4,5). Luffa aegyptiaca (Mill) is found mostly in tropical Africa including Nigeria and some parts of India (6,3,7). Luffa aegyptiaca is commonly found around drainage channels, on dumpsites and uncompleted buildings clinging to nearby objects for support.…”

Section: Introductionmentioning

confidence: 99%

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EX-SITU Characterization of Luffa aegyptaica in Lagos State, Nigeria

et al. 2020

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Luffa aegyptiaca is a plant of multi-purpose importance whose usefulness cuts across virtually all areas of life. This study has characterized L. aegyptiaca in Lagos state and determined the mineral, proximate, phytochemical as well as the heavy metal accumulation potential. Samples were collected from the 20 Local Government Areas (LGAs) in Lagos state at 2 samples per location. Genetic similarity and intra-specific variation in 40 samples of L. aegyptiaca were determined using 3 Random Amplified Polymorphic DNA (RAPD) primers which yielded a total of 42 markers of which 25 was polymorphic. The maximum number of bands (14) was produced by OPC4 while the minimum (7) were produced by OPAF20. Percentage polymorphisms were 70% (OPAF20), 82.4% (OPC4) and 68.4% (OPC6) with an average value of 73.6%. The result from a genetic diversity study was scored to generate a dendrogram using NTsys (2.0j). Phytochemical, proximate, Mineral and the heavy metal study showed the presence of Flavonoid, Saponin; Carbohydrate, protein; Sodium (Na), Calcium (Ca) and Chromium, Iron, Copper, Zinc, Lead among others. The nutritional composition and the potentials of the RAPD marker in distinguishing intra-specific variation in Luffa aegyptiaca were highlighted in this study.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

EX-SITU Characterization of Luffa aegyptaica in Lagos State, Nigeria

et al. 2020

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“…EDITED BY PE Rajasekharan, Indian Institute of Horticultural Research (ICAR), India 2017). L. acutangula and L. cylindrica are annual vines bearing hermaphrodite, staminate, or pistillate flowers (Kumari et al, 2019). Ridge gourd, a member of the Luffa genus, exhibits diverse sex forms, including androecious, andromonoecious, gynoecious, gynomonoecious, monoecious, and hermaphrodite (Choudhary et al, 2011).…”

Section: Open Accessmentioning

confidence: 99%

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

Nagar,

Gowthami,

Sureja

et al. 2023

Front. Plant Sci.

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This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and −20°C) and duration. Cryopreserved pollen at −196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts.

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“…The panelists were asked to rank each dish for color, aroma, texture, bitterness, and the overall taste using three levels of preferences (1: least; 2: moderate; 3: highest). Except for the bitterness, 3 means the highest preference, and for the bitterness, 3 was the highest felt bitterness [18].…”

Section: Variation Of the Photosynthesis Between Field-andmentioning

confidence: 99%

Photosynthetic Phenomics of Field- and Greenhouse-Grown Amaranths vs. Sensory and Species Delimits

et al. 2021

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Consumers hesitate to purchase field-grown shoot-tops of amaranths in Sri Lanka, citing the low-cleanliness making growers focus on greenhouse farming. However, the photosynthetic and growth variations in relation to the organoleptic preference of the greenhouse-grown amaranths in comparison to field-grown counterparts have not been studied. Also, the species delimits of the amaranths in Sri Lanka have not been identified, limiting our ability to interpret species-specific production characteristics. Thus, we assessed the common types of amaranths under greenhouse and field conditions. The photosynthesis was measured using a MultispeQ device of the PhotosynQ phenomic platform, which records chlorophyll fluorescence-based parameters. The shoot-tops were harvested and prepared as dishes according to the typical recipe for amaranths in Sri Lanka. The dishes were subjected to an organoleptic assessment for the parameters color, aroma, bitterness, texture, and overall taste. The differences in plant and the shoot-top biomass were also assessed. The markers atpB-rbcL, matk-trnT, and ITS were used to define the species delimits. The field-grown and greenhouse-grown amaranths exhibited species/cultivar-specific photosynthetic variations. The texture and overall taste of the dishes were different among greenhouse and field-grown material. The tasters preferred the texture and the overall taste of the greenhouse-grown shoot-tops. The greenhouse-grown plants also yielded higher shoot-top harvests compared to field-grown counterparts. Out of the tested markers, ITS defines the delimits of amaranth species. The higher organoleptic preference, the appreciable yield levels, unique photosynthetic patterns of the greenhouse-grown amaranths, and species definitions provide the much-needed platform for clean shoot-top production guaranteeing the highest end-user trust.

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The establishment of the species-delimits and varietal-identities of the cultivated germplasm of Luffa acutangula and Luffa aegyptiaca in Sri Lanka using morphometric, organoleptic and phylogenetic approaches

Cited by 13 publications

References 35 publications

EX-SITU Characterization of Luffa aegyptaica in Lagos State, Nigeria

EX-SITU Characterization of Luffa aegyptaica in Lagos State, Nigeria

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

Photosynthetic Phenomics of Field- and Greenhouse-Grown Amaranths vs. Sensory and Species Delimits

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