The estimation of catecholamines in human plasma

Holly, Jeffrey M P; Makin, H. L. J.

doi:10.1016/0003-2697(83)90374-3

Cited by 82 publications

(25 citation statements)

References 168 publications

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“…Another advantage of the current integrated strategy is that, due to the high specificity provided by UPLC-MS/MS, a simple protein precipitation procedure can be used for plasma sample cleanup, which enables the sample preparation steps (including derivatization) to be carried out in acidic conditions, and therefore could minimize the potential oxidation of E and NE in the sample preparation process. Other reported HPLC-EC methods for simultaneous measurement of plasma E and NE require extensive sample preparation using alumina and boric acid isolation at the basic pH conditions to concentrate samples and/or minimize potential interference from endogenous and exogenous compounds in plasma [12,35]. It has been reported that the rate of E oxidation is much higher at basic condition (e.g., pH 8) than that at low pH [14,15].…”

Section: Chromatography Optimization For Lc-mrm Analysis Of Derivatizmentioning

confidence: 99%

“…doi:10.1016/j.aca.2010.04.051 commonly requires extensive sample preparation in addition to an extended LC separation time (typically more than 10 min) to minimize potential interference from a large number of endogenous and exogenous compounds in biological fluids. The most common sample preparation methods are based on utilization of alumina [12] or boric acid isolation [13], both of which require basic pH (∼8.5) conditions for plasma samples to maximize the extraction efficiency. However, the accuracy of the measurement of plasma E and NE might be compromised because E and NE are readily oxidized under basic conditions [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of plasma epinephrine and norepinephrine using an integrated strategy of a fully automated protein precipitation technique, reductive ethylation labeling and UPLC–MS/MS

Walton

et al. 2010

Analytica Chimica Acta

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Section: Chromatography Optimization For Lc-mrm Analysis Of Derivatizmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of plasma epinephrine and norepinephrine using an integrated strategy of a fully automated protein precipitation technique, reductive ethylation labeling and UPLC–MS/MS

Walton

et al. 2010

Analytica Chimica Acta

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“…16 Moreover, the blood sample was also analyzed for norepinephrine using high-performance liquid chromatography. 17 Because short-term bouts of exercise caused hemoconcentration, 8 postexercise vWF antigen and activity, soluble P-selectin, and norepinephrine levels in plasma were normalized with the changes in hematocrit after exercise. Additionally, analysis of these parameters showed a high reproducibility and a small intra-assay variation in replicates of all tested samples, ie, coefficient variation value in vWF antigen was 8.2%; vWF activity, 6.7%; soluble P-selectin, 9.5%; norepinephrine, 10.1%.…”

Section: Plasma Vwf Antigen and Activity Soluble P-selectin And Normentioning

confidence: 99%

Effects of Exercise Training and Deconditioning on Platelet Aggregation Induced by Alternating Shear Stress in Men

Chen

2005

ATVB

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Objective-Alternating shear stress, which resembles the flow condition in stenotic arteries, induces platelet aggregation.This study investigated how exercise training and deconditioning influence alternating shear-induced platelet aggregation (ASIPA) and clarify the mechanisms underlying ASIPA. Methods and Results-Thirty healthy male sedentary subjects were randomly divided into control and trained groups. The trained men were trained on a bicycle ergometer at Ϸ60% of maximal oxygen consumption for 30 minutes per day, 5 days per week for 8 weeks, and then were deconditioned for 8 weeks. The experimental results indicate the following:(1) short-term strenuous exercise increases the extent of ASIPA and is accompanied by increased the von Willebrand factor (vWF) binding and P-selectin expression on platelets in both the control and trained groups, whereas the enhancement of platelet function decreases after exercise training in trained subjects; (2) at rest and immediately after exercise, ASIPA and the vWF binding and P-selectin expression on platelets are reduced by training, but remain unchanged in the control group; and (3) Key Words: training Ⅲ detraining Ⅲ shear stress Ⅲ platelets Ⅲ adhesion molecules L ifestyle habits such as exercise may have significant influence on the risk of major vascular thrombotic events. 1,2 Previous studies have suggested that the risk of primary cardiac arrest transiently increases during vigorous exercise, 3,4 whereas regular exercise is associated with an overall decreased risk of cardiovascular diseases. 5,6 Shear-induced platelet aggregation (SIPA) is important in arterial thrombosis, which is a major contributing factor for atherothrombotic occlusion of blood vessels. 7 Therefore, it is important to distinguish the shearmediated thrombotic events that occur between short-term bouts of exercise and physical conditioning. According to our earlier study of male patients with stable angina, strenuous acute exercise can increase the capacity of adhered platelets to withstand physiological flow shear stress. 8 A previous investigation also demonstrated that SIPA increased after strenuous treadmill exercise in patients with effort angina. 9 However, the effects of exercise training and detraining on shearmediated platelet activation and its underlying mechanisms have not yet been studied. See page 265Pathological, high shear stress induces binding of von Willebrand factor (vWF) to the platelet glycoprotein (GP) Ib complex on platelets. This interaction transduces signals in platelets, subsequently activating GP IIb/IIIa complex. The activated GP IIb/IIIa complex then binds to fibrinogen, stabilizing the aggregated platelets. 10,11 Our recent investigation showed that short-term intense exercise promoted SIPA, possibly by improving the ability of vWF to bind to platelets and the subsequent activation of GPIIb/IIIa complexes to sustained high shear stress. 12 In fact, blood flow in stenotic vessel follows a complex pattern of hydrodynamics in that flow rate first increases dramatical...

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“…Assessment of catecholamines was generally performed by using liquid chromatography with electrochemical detection, UV absorption and fluorescence detection after derivatization [37,[45][46][47]. Due to the unique advantages of CE in bioanalysis, determination of catecholamines in biological samples with CE has been demonstrated by using various detection techniques [48][49][50][51][52][53][54].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Liu

Chen

2004

Journal of Chromatography B

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The estimation of catecholamines in human plasma

Cited by 82 publications

References 168 publications

Simultaneous determination of plasma epinephrine and norepinephrine using an integrated strategy of a fully automated protein precipitation technique, reductive ethylation labeling and UPLC–MS/MS

Simultaneous determination of plasma epinephrine and norepinephrine using an integrated strategy of a fully automated protein precipitation technique, reductive ethylation labeling and UPLC–MS/MS

Effects of Exercise Training and Deconditioning on Platelet Aggregation Induced by Alternating Shear Stress in Men

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

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