The Ethanol Extract of Polish Propolis Exhibits Anti-Proliferative and/or Pro-Apoptotic Effect on HCT 116 Colon Cancer and Me45 Malignant Melanoma Cells In Vitro Conditions.

Abstract

Background. Propolis is a natural product widely consumed in folk medicine. Different biological activities, such as anticancer, antioxidant, anti-inflammatory, antibiotic and antifungal effects have been reported for propolis and its constituents.Objectives. An in vitro study focused on an evaluation of the biological activity of EEPP, including its anti-proliferative influence on selected neoplastic cells, considering qualitative-quantitative chemical characterization of Polish propolis. Material and Methods. Cytotoxicity was evaluated by means of the MTT and LDH assays. The apoptosis was determined using fluorescence microscopy with annexin V-FITC. Additional EEPP composition was analyzed by a High Performance Liquid Chromatography (HPLC) method. The antimicrobial activity was evaluated by minimal inhibitory concentrations (MIC) against Streptococcus aureus, Enetecoccus faecalis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.

Turkish propolis supresses MCF-7 cell death induced by homocysteine

“…Although, previous studies have represented the effectiveness of propolis that inhibits cancer cells proliferation [35,58], our findings which have indicated protective effects of propolis in Hcyinduced MCF-7 cells likely contribute to incompatible results in cancer studies. Fortunately, we are not alone, Davison et al [62] and Park [63] have also shown conflicting data about the reformative effects of antioxidants on cancer cells opposite to traditional aspect is that antioxidant should be curative to lower cancer risks by counteracting cell-damaging, cancer-causing free radicals.…”

“…According to previous findings, propolis has ability to show various biological activities on different diseases and cells [28,29,49,58]. Actually, the chemical composition could be decisive to determine its biological activities [47,59].…”

Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa

“…Recently, the antimicrobial activity of polish propolis against a P. aeruginosa strain has been reported [27]. Propolis antibacterial property has been attributed to phenolic compounds, especially flavonoids, phenolic acids, and their esters [28].…”

Therapeutic Properties of Bioactive Compounds from Different Honeybee Products

“…New Zealand propolis and its constituents chrysin, galangin, CAPE, benzyl ferulate, benzyl isoferulate, pinostrobin, 5-phenylpenta-2,4-dienoic acid, and tectochrysin, have exhibited antiproliferative effects on DLD-1 colon cancer, HCT-116 colon carcinoma, KYSE-30 esophageal squamous cancer, and NCI-N87 gastric carcinoma cells (Catchpole et al, 2015). Polish propolis rich in phenolic acids and flavonoids has been shown to possess dose-dependent antiproliferative and proapoptotic activities on HCT 116 colon cancer and Me45 malignant melanoma cells (Kubina et al, 2015). …”