The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating Tailless

Chen, Rui; Deng, Xiaobing; Zhu, Sijun

doi:10.1371/journal.pgen.1009928

“…Very few directs targets of Pnt have been identified in Drosophila tissues thus far, including pros [ 18 ], hh [ 21 ], tailless ( tll ) [ 30 ], string ( stg ) [ 19 ], sv [ 22 ] and ETS-domain lacking ( edl ) [ 31 ]. Among these, only pros , hh , stg and sv have been identified as direct Pnt targets in the eye.…”

Section: Resultsmentioning

confidence: 99%

Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed

Bollepogu Raja,

Yeung,

Shim

et al. 2024

BMC Genomics

3

0

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The Ets domain transcription factors direct diverse biological processes throughout all metazoans and are implicated in development as well as in tumor initiation, progression and metastasis. The Drosophila Ets transcription factor Pointed (Pnt) is the downstream effector of the Epidermal growth factor receptor (Egfr) pathway and is required for cell cycle progression, specification, and differentiation of most cell types in the larval eye disc. Despite its critical role in development, very few targets of Pnt have been reported previously. Here, we employed an integrated approach by combining genome-wide single cell and bulk data to identify putative cell type-specific Pnt targets. First, we used chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq) to determine the genome-wide occupancy of Pnt in late larval eye discs. We identified enriched regions that mapped to an average of 6,941 genes, the vast majority of which are novel putative Pnt targets. Next, we integrated ChIP-seq data with two other larval eye single cell genomics datasets (scRNA-seq and snATAC-seq) to reveal 157 putative cell type-specific Pnt targets that may help mediate unique cell type responses upon Egfr-induced differentiation. Finally, our integrated data also predicts cell type-specific functional enhancers that were not reported previously. Together, our study provides a greatly expanded list of putative cell type-specific Pnt targets in the eye and is a resource for future studies that will allow mechanistic insights into complex developmental processes regulated by Egfr signaling.

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“…Very few directs targets of Pnt have been identi ed in Drosophila tissues thus far, including pros(18), hh (21), tailless (tll) (30), string (stg) (19), sv (22) and ETS-domain lacking (edl) (31). Among these, only pros, hh, stg and sv have been identi ed as direct Pnt targets in the eye.…”

Section: Pnt Chip-seq Identi Es Peaks Near Previously Identi Ed Targetsmentioning

confidence: 99%

Integrative Genomic Analyses Reveal Putative Cell Type-specific Targets of the Drosophila Ets Transcription Factor Pointed

Raja,

Yeung,

Shim

et al. 2023

Preprint

0

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The Ets domain transcription factors direct diverse biological processes throughout all metazoans and are implicated in development as well as in tumor initiation, progression and metastasis. The Drosophila Ets transcription factor Pointed (Pnt) is the downstream effector of the Epidermal growth factor receptor (Egfr) pathway and is required for cell cycle progression, specification, and differentiation of most cell types in the larval eye disc. Despite its critical role in development, very few targets of Pnt have been reported previously. Here, we employed an integrated approach by combining genome-wide single cell and bulk data to identify putative cell type-specific Pnt targets. First, we used chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq) to determine the genome-wide occupancy of Pnt in late larval eye discs. We identified enriched regions that mapped to an average of 6,941 genes, the vast majority of which are novel putative Pnt targets. Next, we integrated ChIP-seq data with two other larval eye single cell genomics datasets (scRNA-seq and snATAC-seq) to reveal 157 putative cell type-specific Pnt targets that may help mediate unique cell type responses upon Egfr-induced differentiation. Finally, our integrated data also predicts cell type-specific functional enhancers that were not reported previously. Together, our study provides a greatly expanded list of putative cell type-specific Pnt targets in the eye and is a resource for future studies that will allow mechanistic insights into complex developmental processes regulated by Egfr signaling.

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The Drivers of Diversity: Integrated genetic and hormonal cues regulate neural diversity

Hamid

¹

,

Gutierrez

²

,

Munroe

³

et al. 2023

Seminars in Cell & Developmental Biology

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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating Tailless

Cited by 4 publications

References 57 publications

Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed

Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed

Integrative Genomic Analyses Reveal Putative Cell Type-specific Targets of the Drosophila Ets Transcription Factor Pointed

The Drivers of Diversity: Integrated genetic and hormonal cues regulate neural diversity

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