The evaluation of a small-diameter polysaccharide-based arterial graft in rats

Chaouat, Marc; Visage, Catherine Le; Autissier, Aude; Chaubet, Frédéric; Letourneur, Didier

doi:10.1016/j.biomaterials.2006.06.032

Cited by 75 publications

(66 citation statements)

References 26 publications

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“…We have previously reported the biocompatibility of STMP cross-linked polymer scaffolds in vitro and in vivo in rats. [28][29] The new process by crosslinking with STMP and progressive evaporation, described here for the first time, constitutes an original procedure that endows the PVA with very particular features. The mechanisms of the STMP cross-linking reaction for hydroxylated polymers, such as polysaccharides, have been recently described by Lack et al [30] Phosphoesterification of hydroxyl residues is the main reaction, leading to the formation of phosphoester bridges and phosphate hanging groups, which are negatively charged, favoring a high hydration.…”

Section: Discussionmentioning

confidence: 99%

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

Chaouat

Visage

Baille

et al. 2008

Adv Funct Materials

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173

159

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Poly(vinyl alcohol) (PVA) is a water‐soluble synthetic polymer with excellent film‐forming, emulsifying, and adhesive properties. The aim of this study is to design a simple process for PVA cross‐linking with sodium trimetaphosphate to form membrane devices suitable for biomedical applications. This procedure requires no organic solvent, nor melting process to obtain films with high mechanical strength. Fabrication of a small diameter tube from a PVA film is easy with a single wrapping step around a Teflon rod. Dynamic mechanical analysis demonstrated that, upon removal of the applied stress, the PVA film with a Young's modulus of 2 × 105 kPa returns to its original size and shape. The wall thickness of PVA tubes is 344 ± 13 µm (n = 12), which is close to the wall thickness of a human artery (350–710 µm). Suture retention of a PVA tube is excellent (140 ± 11 g), close to that of human vessels. The burst pressure of PVA tubes is found to be 507 ± 25 mm Hg, more than three times higher than the human healthy systolic arterial pressure. Under arterial pressure, there was no leakage even after needle puncture, contrary to clinical vascular expanded polytetrafluoroethylene prostheses. Finally, PVA tubes of 2 mm in diameter are used to replace a segment of an infrarenal aorta in rats. For at least one week, no mechanical nor thrombotic complications are noticed even in the absence of anticoagulant or antiplatelet treatment. Graft patency is also evidenced with non‐invasive imaging techniques. As a conclusion, this novel cross‐linking method confers to poly(vinyl alcohol) particular mechanical properties such as compliance, elasticity and resistance to mechanical stress, compatible with the circulatory blood flow.

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Section: Discussionmentioning

confidence: 99%

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

Chaouat

Visage

Baille

et al. 2008

Adv Funct Materials

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173

159

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“…Lors de la phase d'application in vivo, les contraintes seront majeures car, après avoir pu insérer le tube vasculaire (sutures, colles, etc. [22,23]), il est en effet nécessaire à la fois d'assurer la fonction de transport du sang et de favoriser la colonisation tout en conservant les propriétés mécaniques. Idéalement, le constituant initial du support doit se dégrader, et ceci sans conséquences défavorables pour les tissus comme par exemple une inflammation excessive.…”

Section: Régénération Vasculaireunclassified

“…Il faut donc choisir des polymères biocompatibles qui ne soient pas thrombogènes. Certains des biomatériaux vasculaires sont donc conçus à partir de polymères biodégradables de type polysaccharidique (dextrane, pullulane, héparine, chitosane) et préparés sans solvant organique et peuvent prendre la forme de tubes de petits diamètres [22]. Le chitosane est ainsi largement utilisé comme hydrogel dans le domaine biomédical pour sa biorésorbabi-lité, sa biocompatibilité et son pouvoir bactériosta-tique.…”

Section: Régénération Vasculaireunclassified

“…Des essais sont en cours chez la brebis pour l'évaluation de ces prothèses vasculaires. Les prothèses vasculaires de 2 mm à base de pullulane/ dextrane montrent chez le petit animal un taux élevé (75 %) de greffons fonctionnels à deux mois [22]. La nature des matériaux polysaccharidiques et l'absence de solvants organiques lors de la préparation semblent garantir une bonne tolérance de l'ensemble.…”

Section: Régénération Vasculaireunclassified

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Ingénierie tissulaire

Letourneur

Bordenave

2017

Med Sci (Paris)

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“…For example, PD scaffolds were chosen in this study for its unique crosslinking mechanism that occurs in ambient and aqueous conditions. 24 This can potentially provide more physiologically relevant substrates for in vitro and in vivo studies.…”

mentioning

confidence: 99%

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Cutiongco

Teo

Yim

2015

JoVE

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Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche. Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors. The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery. Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold. We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules. We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior. Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche. Video LinkThe video component of this article can be found at

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The evaluation of a small-diameter polysaccharide-based arterial graft in rats

Cited by 75 publications

References 26 publications

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

A Novel Cross‐linked Poly(vinyl alcohol) (PVA) for Vascular Grafts

Ingénierie tissulaire

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

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