The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury

Abstract

This investigation aimed to evaluate the defensive impacts of citral on ischemia/reperfusion (I/R) injury induced by testicular torsion/detorsion (T/D) in rats in an experimental model. The grouping of subjects was as follows: (1) sham, (2) T/D, (3) and (4) T/D plus citral 150 and 300 mg/kg, respectively, and (5) intact (citral 300 mg/kg). T/D was performed by testicular 720° turning for 2 h and then detorsion for 24 h. Blood serum was obtained to assess testosterone and oxidative stress markers, epididymal sperms were collected for sperm staining and sperm analysis, and testicular tissues were examined for histopathology. T/D damage was associated with a remarkable decline in sperm total count, viability, and some velocity parameters in comparison to the sham group (p < 0.05), which could be reversed significantly by citral (p < 0.05). Histopathologically, T/D damage caused severe oedema, haemorrhage, inflammation, and seminiferous tubules disruption, while citral improved significantly the mean seminiferous tubular diameter, Cosentino's score, and Johnsen's score (p < 0.05). I/R injury was associated with significant increased malondialdehyde and oxidative stress index, and also significant reduced total antioxidant capacity and testosterone versus the sham group (p < 0.05), which all were prevented significantly by citral administration (p < 0.05). The outcomes greatly proved that testicular I/R injury could be significantly prevented by citral.

Evaluation of semen DNA integrity and related parameters with COVID-19 infection: a prospective cohort study

“…In a study on the treatment of varicocele-induced decline in semen quality with medication, Melissa officinalis was found to effectively improve sperm count, motility, and chromatin structure [ 32 ], indicating the protective effect of antioxidant on the male reproductive system [ 33 ]. Similarly, in the treatment of a unilateral testicular ischemia reperfusion injury model, citral demonstrated a powerful protective effect [ 34 ], displaying strong anti-inflammatory and antioxidant effects. This also suggests that anti-inflammatory and antioxidant therapies may have a positive role in the treatment of COVID-19 patients.…”