The evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens

Kısa, Özgül; Albay, Ali; Mas, Mehmet Refik; Celasun, Bülent; Doğancı, Levent

doi:10.1016/s0732-8893(02)00409-1

Cited by 25 publications

(25 citation statements)

References 18 publications

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“…Of these, 4 were used for histological analysis. The other 6 biopsies were subjected to urease testing and PCR (Coelho and Zaterka, 2005;Kisa et al, 2002). The samples were maintained in brain-heart infusion broth containing 20% glycerol and were stored at −70°C until DNA extraction.…”

Section: Clinical Samplementioning

confidence: 99%

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Vianna

Ramis

Halicki

et al. 2015

Diagnostic Microbiology and Infectious Disease

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Section: Clinical Samplementioning

confidence: 99%

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Vianna

Ramis

Halicki

et al. 2015

Diagnostic Microbiology and Infectious Disease

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“…A combined analysis of H&E and IHC stains, in which results were positive if either test was positive or results were negative if both tests were negative, did not improve sensitivity while the number of false positive test results increased. Culture of H. pylori in gastric biopsy specimens has very high specificity but relatively low sensitivity [5,32]. In the present study, culture provided 100% specificity and 82% sensitivity.…”

Section: Discussionmentioning

confidence: 50%

“…Overall, NSAID use did not seem to influence H. pylori eradication rates or serological testing for H. pylori IgG-antibodies, when compared to other studies with patients who do not take NSAIDs [5,32]. Although studies on H. pylori IgG serology are not new, there is some data available in which has been shown that NSAID treatment significantly decreases the degree of gastric inflammation [22-25].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Helicobacter pylorieradication in patients on NSAID treatment

et al. 2012

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BackgroundIn this post-hoc analysis of a randomized, double blind, placebo controlled trial, we measured the sensitivity and specificity of Helicobacter pylori IgG-antibody titer changes, hematoxylin and eosin (H&E) stains, immunohistochemical (IHC) stains and culture results in NSAID using patients, following H. pylori eradication therapy or placebo.Methods347 NSAID using patients who were H. pylori positive on serological testing for H. pylori IgG-antibodies were randomized for H. pylori eradication therapy or placebo. Three months after randomization, gastric mucosal biopsies were taken for H. pylori culture and histological examination. At 3 and 12 months, blood samples were taken for repeated serological testing. The gold standard for H. pylori infection was based on a positive culture or both a positive histological examination and a positive serological test. Sensitivity, specificity and receiver operating curves (ROC) were calculated.ResultsH. pylori eradication therapy was successful in 91% of patients. Culture provided an overall sensitivity of 82%, and 73% after eradication, with a specificity of 100%. Histological examination with either H&E or IHC stains provided sensitivities and specificities between 93% and 100%. Adding IHC to H&E stains did not improve these results. The ROC curve for percent change in H. pylori IgG-antibody titers had good diagnostic power in identifying H. pylori negative patients, with an area under the ROC curve of 0.70 (95 % CI 0.59 to 0.79, P = 0.085) at 3 months and 0.83 (95% CI 0.76 to 0.89, P < 0.0001) at 12 months. A cut-off point of at least 21% decrease in H. pylori IgG-antibody titers at 3 months and 58% at 12 months provided a sensitivity of 64% and 87% and a specificity of 81% and 74% respectively, for successful eradication of H. pylori.ConclusionsIn NSAID using patients, following H. pylori eradication therapy or placebo, histological examination of gastric mucosal tissue biopsies provided good sensitivity and specificity ratios for evaluating success of H. pylori eradication therapy. A percentual H. pylori IgG-antibody titer change has better sensitivity and specificity than an absolute titer change or a predefined H. pylori IgG-antibody titer cut-off point for evaluating success of H. pylori eradication therapy.

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“…This concept is further corroborated by the increased inflammation in these patients. The superior sensitivity of PCR to detect H. pylori in gastric biopsy compared with conventional histology and bacterial culture has been described, 10,15,21,23,24,27,28,49 but cross-contamination during the PCR procedure has been suspected. 10,20,25 Additional, noninvasive tests, including serology and stool antigen detection, may support the PCR findings.…”

Section: Discussionmentioning

confidence: 99%

“…13,48 A sensitivity of 77% to 99% has been reported for the detection of H. pylori by histology. 15,23,28,30,37,45,49 Auxiliary methods, including immunohistochemistry, 2,18,30 in situ hybridization 5,22 and the polymerase chain reaction (PCR), 2,9,46,49 have been applied to improve the sensitivity to detect H. pylori in biopsy material. 47 Besides the direct visualization of the bacteria, a characteristic pattern of inflammation including neutrophils, mononuclear cells, as well as lymphoid follicles can be observed by histology; albeit only the detection of the bacteria unequivocally proves the diagnosis.…”

mentioning

confidence: 99%

Increased Rate of Helicobacter pylori Infection Detected by PCR in Biopsies With Chronic Gastritis

Zsikla¹,

Hailemariam²,

Baumann³

et al. 2006

American Journal of Surgical Pathology

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Histology is considered a sensitive method for detection of Helicobacter pylori, in gastric biopsies. We investigated the diagnostic potential of qualitative nested (nPCR) and quantitative PCR (qPCR) for detection of H. pylori using different primers on 126 archived gastric biopsies with inflammation and correlated the inflammatory changes with the presence and density of bacteria. H. pylori was detected in 42.8% biopsies by histology and PCR, an additional 15 samples were positive exclusively by PCR: nPCR was positive in all histologically positive samples, but qPCR failed to detect H. pylori in 10 biopsies. The inflammatory score was significantly higher in biopsies positive for H. pylori only by PCR showed a significant higher inflammatory score compared with negative biopsies (mean of neutrophils score, 1.60 vs. 0.90, P < 0.01; mean of mononuclear cells score, 2.27 vs. 1.67, P < 0.01), whereas the inflammatory score was similar compared with biopsies positive for H. pylori by histology (mean of neutrophils score, 1.60 vs. 1.56, not significant; mean of mononuclear cells score, 2.27 vs. 2.20, not significant). A weak correlation between inflammatory score and the density of H. pylori detected by histology was observed. The mean values of H. pylori DNA were significantly higher in histologic-positive than in histologic negative biopsies. We have shown that PCR can detect H. pylori in about 20% of histologic-negative gastric biopsies, indicating the clinical relevance of H. pylori detection by PCR in biopsies with characteristic inflammatory changes.

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The evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens

Cited by 25 publications

References 18 publications

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Assessment of Helicobacter pylorieradication in patients on NSAID treatment

Increased Rate of Helicobacter pylori Infection Detected by PCR in Biopsies With Chronic Gastritis

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