The Evaluation of Free Thyroid Hormones (FT4 and FT3) in the Routine Diagnosis of Thyroid Function

Abstract

SummaryThe validity of the free thyroid hormone parameters (FT4 and FT3) was verified in a random sample of 154 ambulatory patients with thyroid conditions. The “euthyroid range” of FT4 was between 15.67 and 30.66 pmol/1; median 21.98 pmol/1. The distribution of the FT4 readings peaked on the left and sloped to the right (log normal). In our laboratory, the “euthyroid reference range” of FT4 is between 10-28 pmol/1. The “euthyroid range” of FT3 extended from 4.6 to 9.7 pmol/ 1; median 6.63 pmol/1. The distribution of the readings was likewise log normal. The values of FT4 and FT3 are not significantly influenced by TBG concentration anomalies in otherwise healthy thyroid patients. For purposes of discrimination between euthyroidism and hyperthyroidism, FT3 (95%) and FT4 (90%) are better suited than the corresponding quotients for the free hormone fraction or the total hormone concentrations. On the other hand, the free hormone parameters are less suitable for the diagnosis of hypothyroidism. These results were deduced theoretically from mathematical function analyses between the TBG-independent free hormone parameters and the TBG-dependent hormone concentrations.

Thyroid hormones regulate cardiac repolarization and QT-interval related gene expression in hiPSC cardiomyocytes

“…To analyze the effects of THs on hiPSC-CMs at doses near to physiological levels (FT3 euthyroid range: 4.6 to 9.7 pmol/l, median 6.63 pmol/l. ; FT4 euthyroid range: 15.67 and 30.66 pmol/l; median 21.98 pmol/l) 29 , cells were treated with lower hormone concentrations in a serum-free medium for 24 h. The hiPSC-CMs responsiveness to E4031 and Nifedipine in this medium was confirmed (Supplementary Fig. 1b).…”