The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis

Bergbrant, Ing‐Marie; Johansson, S. G. O.; Robbins, D. S.; Bengtsson, Karin; Faergemann, Jan; Scheynius, Annika; Söderström, T

doi:10.1111/j.1365-2230.1991.tb00396.x

Cited by 20 publications

(9 citation statements)

References 13 publications

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“…17 Bergbrant et al found no difference between Malassezia-specific antibody levels in such groups with four of five assay methods, although one of their assays showed a marginally reduced antibody level in the patient group. 18 Kieffer et al found no evidence of IgE antibody to Malassezia in seborrhoeic dermatitis and only occasional reactions in healthy controls. 19 Ashbee et al found that total blood lymphocyte counts in patients with seborrhoeic dermatitis were indistinguishable from those in healthy subjects, as were the proportion of their lymphocytes expressing CD4 and CD8 markers, the numbers of circulating B cells, and the levels of total serum immunoglobulins.…”

Section: Discussionmentioning

confidence: 97%

“…Malassezia-specific antibody can be detected in the sera of all adults but the quantity is very variable. [16][17][18][19][20] Midgley and Hay found a slightly greater mean level of Malassezia-specific IgG in patients with seborrhoeic dermatitis than in healthy subjects. 16 Ashbee et al found no difference in total Malasseziaspecific antibody, or immunoglobulin subclasses, between patients with seborrhoeic dermatitis and controls.…”

Section: Discussionmentioning

confidence: 99%

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Seborrhoeic dermatitis is not caused by an altered immune response toMalasseziayeast

Parry

Sharpe

1998

British Journal of Dermatology

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The immune response of patients with seborrhoeic dermatitis and healthy age- and sex-matched controls was examined to test the hypothesis that an inadequate or inappropriate immune response to Malassezia yeast leads to seborrhoeic dermatitis. Antibody responses were examined using enzyme-linked immunosorbent assays (ELISAs) and Western blots and lymphocyte responses using lymphocyte proliferation assays. The level of IgG and IgM specific for whole yeast cells or extracted proteins of two isolates of M. furfur was tested in ELISA. A wide range of antibody levels was found but the patient and control groups were indistinguishable (n = 19), and the groups could not be distinguished by the pattern of Malassezia proteins recognized by their sera in Western blots. The average affinity of the subjects' antibodies specific for Malassezia cells or proteins was measured using ammonium thiocyanate dissociation. Most of the sera had moderate affinities corresponding to 50% dissociation at thiocyanate concentrations of 0.5-1.0 mol/L. There was no difference between patients and matched controls. The proliferation of the patients' lymphocytes in response to a number of M. furfur cell preparations was measured: whole cells, cytoplasmic proteins, cell walls, soluble molecules extracted from the cell walls using sonication, and a commercial preparation. There was a wide range of responses between individuals, but there was no difference between the three groups: patients with seborrhoeic dermatitis (n = 16), healthy controls (n = 16) and a group suffering from other inflammatory skin conditions (n = 15). The results do not support the hypothesis that an inadequate immune response to Malassezia yeast could lead to seborrhoeic dermatitis. Other possible pathological mechanisms include toxin production or lipase activity.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Seborrhoeic dermatitis is not caused by an altered immune response toMalasseziayeast

Parry

Sharpe

1998

British Journal of Dermatology

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“…12 The humoral immune response to Malassezia in patients with seborrhoeic dermatitis and pityriasis versicolor has been studied using different antigen preparations and different techniques: elevated titres in patients compared with controls, as well as no differences in titres, have been reported. [13][14][15][16][17][18] A cell-mediated immune response to Malassezia has been demonstrated by lymphocyte transformation tests in healthy individuals. 19 Lymphocyte transformation in patients with pityriasis versicolor and in patients with seborrhoeic dermatitis has been reported with divergent results.…”

Section: Introductionmentioning

confidence: 99%

Cell-mediated immunity to Malassezia furfur in patients with seborrhoeic dermatitis and pityriasis versicolor

Bergbrant

Andersson

Faergemann³

1999

Clinical and Experimental Dermatology

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The lymphocyte transformation response to Malassezia furfur, Candida albicans, phytohaemagglutinin, concanavlin A and tuberculin purified protein derivative of 12 patients with pityriasis versicolor, 15 patients with seborrhoeic dermatitis and matched controls, was studied. Patients with pityriasis versicolor showed a significantly lower response to M. furfur than patients with seborrhoeic dermatitis and controls.

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“…The effect of different methods and different antigen prep- (50). Comparing combinations of various methods and antigens, the only combination able to find a significant difference in antibody titers between SD patients and controls was an ELISA using a cell wall protein antigen.…”

Section: Seborrheic Dermatitis and Dandruffmentioning

confidence: 99%

Immunology of Diseases Associated withMalasseziaSpecies

2002

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SUMMARY Malassezia species are members of the human cutaneous commensal flora, in addition to causing a wide range of cutaneous and systemic diseases in suitably predisposed individuals. Studies examining cellular and humoral immune responses specific to Malassezia species in patients with Malassezia-associated diseases and healthy controls have generally been unable to define significant differences in their immune response. The use of varied antigenic preparations and strains from different Malassezia classifications may partly be responsible for this, although these problems can now be overcome by using techniques based on recent work defining some important antigens and also a new taxonomy for the genus. The finding that the genus Malassezia is immunomodulatory is important in understanding its ability to cause disease. Stimulation of the reticuloendothelial system and activation of the complement cascade contrasts with its ability to suppress cytokine release and downregulate phagocytic uptake and killing. The lipid-rich layer around the yeast appears to be pivotal in this alteration of phenotype. Defining the nonspecific immune response to Malassezia species and the way in which the organisms modulate it may well be the key to understanding how Malassezia species can exist as both commensals and pathogens.

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The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis

Cited by 20 publications

References 13 publications

Seborrhoeic dermatitis is not caused by an altered immune response toMalasseziayeast

Seborrhoeic dermatitis is not caused by an altered immune response toMalasseziayeast

Cell-mediated immunity to Malassezia furfur in patients with seborrhoeic dermatitis and pityriasis versicolor

Immunology of Diseases Associated withMalasseziaSpecies

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