The evaluation of waste, surface and ground water quality using the Allium test procedure

Smaka-Kincl, Vesna; Stegnar, P.; Lovka, Milan; Toman, Mihael J.

doi:10.1016/s0165-1218(96)90059-2

Cited by 212 publications

(112 citation statements)

References 15 publications

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“…Mitotic index is an acceptable measure of cytotoxicity in all living organism (Smaka-Kinel et al 1996). The cytotoxicity level can be determined by the decreased rate of mitotic index.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

Sreeranjini¹,

Siril²

2011

Plant Soil Environ.

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Morinda citrifolia Linn. (family Rubiaceae) is a small tree occurring in tropical areas of the world. The plant contains several medicinally active components that exhibit the therapeutic effects such as antibacterial, antiviral and anticancer activities. Anti-genotoxic effects of aqueous extracts prepared using dried leaves of M. citrifolia was studied. Allium cepa root tip meristem cells treated with 7% hydrogen peroxide were used for eliciting antigenotoxicity. For this purpose experiments were performed with A. cepa onion bulbs treated for 24 h with different concentrations (15 or 30 g/L) of aqueous extract with or without pre-treatment (1 h) with 7% hydrogen peroxide. A significant reduction in mitotic index was recorded in treatment groups over negative control. Chromosomal aberrations such as breaks, bridges, stickiness and polar deviations were observed in positive control and treatment groups. The highest (21.48) percentage of chromosomal aberrations was noticed in positive control. A significant reduction in chromosomal aberrations (9.39) was recorded in root tips treated with hydrogen peroxide followed by 15 g/L extract. H 2 O 2 induced chromosomal aberrations were reduced due to leaf extract treatment indicates antimutagenic potential of the M. citrifolia. The observations suggest that M. citrifolia aqueous leaf extracts have antimitotic and anti-genotoxic effects; consequently oxidative stress induced aberrations due to H 2 O 2 are efficiently restored in the extract treated A. cepa root meristem cells.

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“…Mitotic index is an acceptable measure of cytotoxicity in all living organism (Smaka-Kinel et al 1996). The cytotoxicity level can be determined by the decreased rate of mitotic index.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

Sreeranjini¹,

Siril²

2011

Plant Soil Environ.

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“…Mitotic index used as a pointer of cell proliferation which measures the level of cells in the mitotic stage of the cell cycle. Hence, cellular death could be understood as the reduction in the mitotic index of A. cepa meristematic cells [38] . The mitodepressive effect was frequently used in locating cytotoxicity [39] .…”

Section: Chromosomal Aberrationsmentioning

confidence: 99%

Treated wastewater impact on rural green farm life cycle, Egypt

Awady

Marzouk²,

Bakry

et al. 2017

Egypt. J. Chem.

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71T REATED effluent from wastewater treatment plant (WWTP) offers significant water demand in all areas of activities in rural farm. This research evaluates the reuse of secondary treated wastewater (STWW) for irrigating crops, fresh vegetables and feeding fish farm. Physico-chemical, heavy metals and cytogenetic have been investigated. Intensive analyses of crops seeds, vegetables components, soil, dried sludge and fish organisms have been carried out. Results showed that the characteristics of secondary treated wastewater can be used for crops plantation and fish farming. Cytogenetic studies using Alliums' bio-tests were investigated. The concentrations of each: Cr, Pb, Co, Se, Ni, Cu, and Zn in STWW was less than 0.1 mg/l. Carbon content, nitrogen, and phosphorous were: 6.0; 1.2; and 0.1 mg/l, respectively fulfilling the requirements C: N: P ratios for plant growth. Results showed also that the irrigated crops and vegetables exhibited good growth. Analyses of roots, stems, leaves, seeds and sludge showed metals concentrations were within the permissible range. Tilapia fish showed noticeable vitality. Parasitological examination of fish revealed no parasitic cysts detected in the skin, gills and intestine, respectively. It showed no bacterial strains isolated from fish tissues. Macroscopically parameters as root length and shape look normal and healthy, while microscopically parameters showed no change of mitotic index, RDR, phase index, chromosomal aberrations of the bioassay plant tissues and do not stimulate specific type of chromosomal aberrations as well as percentage of chromosomal aberrations. All studied parameters were found in normal limits when compared with control values. This indicated the efficiency of the treatment system; hence STWW has no mutagenicity or cytotoxicity effects. Examined parameters in this study were found in normal limits if compared with trigger levels of treated wastewater.

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“…Therefore, the inhibition of adenosine triphosphatase by LCT adversly affects cell integrity, and in this case resulted in decreased mitotic index. Smaka-Kincl et al (1996) showed that the decrease in MI of A. cepa meristematic cells can be considered as a easy method to determine the cytotoxicity of agents.…”

Section: MI Mn and Cas Analysismentioning

confidence: 99%

The Effect of Lambda-Cyhalothrin on Root Tip Cytology, Pigment Contents and Antioxidant Defense System of <i>Allium cepa</i>

et al. 2014

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SummaryIn this study, toxic effects of the lambda-cyhalothrin (LCT) in Allium cepa L. cells were investigated. Toxic effects were investigated by analyzing the leaf contents and the cytologic and antioxidant parameters. To this aim, different doses of LCT were applied to Allium cepa L. samples. As a result, all doses of LCT treatment significantly decreased the leaf pigments compared to the control. The activity of superoxide dismutase, catalase and MDA level showed a concentration-time dependent decrease after treatment. It was also found that LCT has a mitodepressive action on mitosis and produced clastogenic and aneugenic types of abnormalities in Allium cepa with decreased mitotic index depending on the dose of LCT. The data obtained in this study showed that plant bioassays can be used as an important indicator to detect possible genotoxicity of chemicals.

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The evaluation of waste, surface and ground water quality using the Allium test procedure

Cited by 212 publications

References 15 publications

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

Treated wastewater impact on rural green farm life cycle, Egypt

The Effect of Lambda-Cyhalothrin on Root Tip Cytology, Pigment Contents and Antioxidant Defense System of <i>Allium cepa</i>

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