The examination of samples infected with multiple salmonella serotypes

Harvey, R. W. S.; Price, T. H.

doi:10.1017/s0022172400045939

Cited by 41 publications

(30 citation statements)

References 22 publications

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“…Some possibilities include serotyping multiple isolates per plate, use of multiple enrichment broths and plating media, culturing multiple samples per pig, or sampling more animals per herd or studied groups. Research on the dynamics of bacterial growth in selective enrichment broths 2,7,8 suggests that selective enrichment may result in asynchronous growth curves among serotypes, because of differing susceptibilities to the restrictive components of the media, with a consequent difference in recovery rates of different serotypes, as demonstrated in our study. Therefore, if only 1 culture method is used, selection of more than 1 colony per sample for identification or sampling more animals per group may not be as efficient as using multiple methods for isolation of different serotypes.…”

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confidence: 64%

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

et al. 2005

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Abstract. Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n ϭ 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities showed that methods B and C performed significantly better (P Ͻ 0.05) than methods A and D. Although relative sensitivities of methods B and C were equal, from the 89 positive samples concomitantly detected by both, 35 (39.3%) had different serotypes (no match) isolated by each method. On the basis of the results of this study, it was concluded that culture methods differ on the isolation of S. enterica serotypes from naturally contaminated swine fecal samples. Depending on the objective(s) of investigations on the ecology and epidemiology of S. enterica in swine populations, a method or a combination of methods should be considered for more reliable results.An incredible number of different culture methods for isolation of Salmonella have been published, but currently, there appears to be no consensus on what would be the best method. 12 As a consequence, confusing and contradictory results are frequently found in the literature.For years, 2 culture methods (methods A and B, described below) have been concomitantly applied in our laboratory routine for isolation of Salmonella from swine samples. 13 A recent study 3 compared these methods with another frequently applied culture method (preenrichment in buffered peptone water, followed by enrichment in Rappaport-Vassiliadis [RV] broth and isolation on Xylose-Lysine-Tergitol-4 [XLT-4] agar), reporting better results when isolation was conducted using method B. However, we felt that continuous im- Disclaimer: Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the U.S. Department of Agriculture. provement in the sensitivity of culture methods was necessary and should be persistently pursued. Therefore, culture methods C and D (described below) were developed and included in this study, whose objective was to comparatively evaluate 4 different culture methods (methods A, B, C, and D) and to assess their relative sensitivity for the isolation of Salmonella enterica from naturally contaminated swine fecal samples. The current knowledge of the effect of different culture methods on the isolation of different S. enterica serotypes from naturally contaminated samples is very limited. Most studies published to date have simply focused on the sensitivity of culture methods for the detection of positive samples presenting little information on the isolation of multiple serotypes. This information is crucial, particularly when trying to understand the complex ecology and epidemiology of Salmonella in livestock.To compare the culture methods for the isolatio...

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confidence: 64%

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

et al. 2005

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“…When examining samples containing multiple salmonella serotypes, use of complementary cultural techniques has been briefly discussed (Harvey, 1957;Harvey & Price, 1967). The present communication records results obtained with two different enrichment broths in routine practice.…”

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confidence: 99%

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Harvey

Price

1976

J. Hyg.

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SUMMARYSelenite F broth and a modified Muller-Kauffmann tetrathionate broth were investigated using sewage-polluted natural water as inocula. The modification of the tetrathionate medium was necessary as commercial alternatives would not allow multiplication of small numbers of salmonellas.The wide range of molar concentrations in different tetrathionate broths was emphasized.Selenite F broth was more efficient than Muller-Kauffmann tetrathionate broth, in our hands, with the material tested. Direct inoculation of the enrichment media was used.Each of the two media examined had a bias towards selection of certain serotypes. If possible, both enrichment broths should be used to obtain maximum information.

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“…From a different point of view, several investigators have reported that a secondary enrichment in selenite broth or tetrathionate broth also tends to increase the yield of salmonellas from food or highly contaminated material (Jameson, 1962(Jameson, , 1963Edel & Kampelmacher, 1968;Martin & Ewing, 1967;Harvey & Price, 1967;and others). In this paper, we report our findings from a comparative evaluation of the isolation of salmonellas from abattoir drains by three different procedures: first, by the use of conventional enrichment media incubated at 370 C.; secondly, by conventional enrichment media incubated at 43°C.…”

Section: Introductionmentioning

confidence: 99%

Salmonella isolations in abattoirs in Greece

et al. 1970

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SUMMARYThe prevalence of salmonellas in drain swabs from three abattoirs of Athens was studied with the use of conventional methods of Salmonella isolation as well as with a new procedure which involves secondary enrichment from the usual selenite broth or Muller-Kauffmann's tetrathionate broth in Rappaport's medium slightly modified.In all groups studied the secondary enrichment in Rappaport's medium led to an increase in the number of positive swabs, in the number of Salmonella serotypes, and in the total number of strains isolated.The frequency of Salmonella isolations was higher in samples from abattoirs killing only pigs and lower in samples from abattoirs killing only cattle or only sheep.The predominant serotype in abattoirs dealing with cattle was Salmonella tennessee, and S. typhimurium in abattoirs dealing with sheep. No predominant serotype was found in samples from abattoirs dealing mostly with pigs.S. abony, S. drypool, S. emek, S. Indiana, S. muenchen and S. tennessee were isolated for the first time in Greece.

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The examination of samples infected with multiple salmonella serotypes

Cited by 41 publications

References 22 publications

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Salmonella isolations in abattoirs in Greece

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