The Exopolysaccharide Produced by Lactobacillus paracasei IJH-SONE68 Prevents and Ameliorates Inflammatory Responses in DSS–Induced Ulcerative Colitis

Abstract

Inflammatory bowel disease (IBD) is an autoimmune disease characterized by chronic inflammation of the gastrointestinal tract. IBD includes Crohn’s disease (CD) and ulcerative colitis (UC). CD can occur in any part of the gastrointestinal tract, whereas UC mainly occurs in the colon and rectum. We previously demonstrated that a novel exopolysaccharide (EPS) produced by a plant-derived bacterium, Lactobacillus paracasei IJH-SONE68, prevents and improves the inflammation in contact dermatitis model mice via oral administration. To evaluate the preventive effect of the EPS against other inflammatory diseases, in the present study, we employed dextran sulfate sodium (DSS)-induced UC model mice. The stool consistency, hematochezia, and colonic atrophy of the mice were improved by the orally administered EPS. We also evaluated the cytokine transcription. Overexpression of the mouse macrophage inflammatory protein 2 mRNA in the colon as a functional homolog of human interleukin-8 was decreased by the orally administered EPS. However, the expression of interleukin-10, which is known as an anti-inflammatory cytokine, was stimulated in the EPS-administrated group. Based on these results, we conclude that the IJH-SONE68-derived EPS is a promising lead material for the development of drugs useful in treating inflammatory diseases such as UC.

Plant-Derived Lactobacillus paracasei IJH-SONE68 Improves Chronic Allergy Status: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

“…Our recent study also showed that the IJH-SONE68-derived EPS prevents and ameliorates inflammatory responses in the DSS-induced ulcerative colitis model mice with significant repression of MIP-2, which is a functional analogue of human IL-8 [27]. The inflammatory cytokine IL-8 has been reported to play an important role during chronic disease progression through binding to IL-8 receptors A and B, located on the neutrophils [35][36][37].…”

“…The precise mechanisms of the IJH-SONE68-derived EPS are not clear yet. However, the EPS seems to modulate excessive immune responses, judging from the result obtained by animal experiments [26,27]. Therefore, by affecting not only IgE-mediated allergic reaction, but also chronic inflammation, the IJH-SONE68-derived EPS might improve the self-assessment questionnaire in the subjects.…”

“…In addition, the IJH-SONE68-derived EPSs inhibit the catalytic activity of hyaluronidase, as well as some commercially used anti-allergic and anti-inflammation agents [15,17,24,25]. Our recent animal experiments also showed that IJH-SONE68-derived EPSs prevent and improve picryl chloride-induced contact dermatitis and dextran sulfate sodium (DSS)-induced ulcerative colitis on model mice, by repressing the accelerated expression of interleukin (IL)-4 and the serum IgE level [26] and by reducing the MIP-2 expression [27], respectively. Therefore, in the present study, we aim to show that IJH-SONE68-derived EPSs improve allergic conditions by conducting a clinical trial involving subjects with perennial allergy symptoms, which may be accompanied by chronic inflammation.…”

Probiotics as Potential Biological Immunomodulators in the Management of Oral Lichen Planus: What’s New?

“…Similarly, the use of L. delbrueckii and L. fermentum for 8 weeks downregulated NF-kB p65 in UC-affected patients, preventing relapses [ 117 ]. Furthermore, exopolysaccharides (EPSs) from L. paracasei IJH-SONE68 improved inflammation in an allergy mice model of contact dermatitis and ameliorated colon atrophy, stool consistency and hematochezia in a UC mice model [ 153 , 154 ].…”

Plant-Derived Lactobacillus paracasei IJH-SONE68 Improves the Gut Microbiota Associated with Hepatic Disorders: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

“…We have also shown that the intake of IJH-SONE68-derived EPS prevents and ameliorates inflammatory responses in both picryl chloride-induced contact dermatitis and dextran sulfate sodium (DSS)-induced ulcerative colitis in model mice. This was achieved via the reduction in the accelerated expression of inflammatory cytokines [ 12 , 13 ]. Our clinical study involving volunteer participants with perennial allergy symptoms also showed that the EPS from the IJH-SONE68 strain improved allergic conditions [ 14 ].…”