The Experimental Research of R-Phycoerythrin Subunits on Cancer Treatment: A New Photosensitizer in PDT

Abstract

The mouse tumor cell S180 and human liver carcinoma cell SMC 7721 cells were first treated with R-PE and its subunits (alpha, beta, gamma subunits), then irradiated with Argon laser (496 nm, 28.8 J/cm2). Survival rate was measured by MTT method. In order to compare the phototoxicity in normal cells, the mouse marrow cells were treated with photofrin II and beta-subunit, irradiated with 45 J/cm2 of light; survival rate was also measured by MTT method. The result showed that R-PE subunits had better PDT effect on s180 cells than R-PE and lower phototoxicity in marrow cells than photofrin II. Flow cytometric analysis showed that PDT results in a growth inhibition and a G0-G1 cell cycle arrest in SMC 7721 cells. The tumor cells inhibited by PDT in vivo were morphologically observed by TEM, the tumor cell death was due to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei. Therefore, with the advantage in special fluorescence activity, low molecular weight, good light absorbent character and weak phototoxicity, R-PE subunit is an attractive option for improving the selectivity of PDT.

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

“…Functional food substances from marine sources are not only pharmacologically effective but also antagonize dysregulated targets in cellular signaling pathways to exert their antineoplastic activities (Tarapore, Siddiqui, & Mukhtar, ). Phycobiliproteins are an important group of water soluble natural pigment molecules extracted from the red and blue green algae and they are nontoxic proteins with potent anticancer, antioxidant, and anti‐inflammatory properties (Bei, Guang‐Ce, Chen‐Kui, & Zhen‐gang, ; Ravi, et al, ; Senthilkumar, Kurinjimalar, et al, ; Thangam, et al, ). Hence, an investigation was carried out to evaluate the anticancer potential of RPERPE obtained from P. hornemannii (Lyngbye) Silva against HCC using male Wistar albino rats as models.…”

Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption

“…They have been used as photosensitizers for the treatment of tumors (Huang et al 2002;Li et al 2000). Recent studies have also shown their applicability in immunomodulation (Román et al 2002).…”

Recent advances in production, purification and applications of phycobiliproteins

“…APC, at nontoxic concentration to the host cells (0.045 ± 0.012 μmol/L), was found to inhibit enterovirus 71 induced cytopathic effects, viral plaque formation, and apoptosis [60] . Bei et al [61] (2002) found the role of PE in improving the selectivity of photodynamic therapy treatment of mouse tumor cells S180 and human liver carcinoma cells SMC 7721. Sonani et al [43,62] (2014a, 2014b) has recently reported the antioxidant based antiaging activity and antiAlzheimeric potential of PE (isolated from Lyngbya sp.…”