The Experimental Study of Polyelectrolyte Coatings Suitability for Encapsulation of Cells

Abstract

Living cells encapsulated in polymeric shells are receiving increasing attention because of their possible biotechnological and biomedical applications. The aim of this work is to evaluate how different polyelectrolyte coatings, characterized by different numbers of polyelectrolyte layers and by different polyelectrolyte conformations, affect the viability of encapsulated biological material. We demonstrate the ability to individually encapsulate HL-60 cells as well as rat pancreatic islets within polymeric shells consisting of different PE layers using the layer-by-layer process. Coating of HL-60 cells allows for surviving and functioning of cells for all applied PE as well as for different numbers of layers. The islets encapsulated in applied polyelectrolytes exhibited the lower level of mitochondrial activity as compared to non-encapsulated islets. Nevertheless, encapsulated islets exhibited comparable absorbance values during the whole period of culture. Polyelectrolyte coating seems to be a promising way of allowing capsule void volume minimization in a model of encapsulated biological material for local production of biologically active substances.

Chitosan‐Based Nanocoatings for Hypothermic Storage of Living Cells

“…We also checked the interactions of CCh and ACh with MTT solution. These experiments confirm that chitosan based nanocoating are considerably better that these prepared from the synthetic polyelectrolytes …”

“…Encapsulation in polymeric beads requires storage of the large sample volumes, which is dominated by the polymer. The preliminary attempts to cover the cells with thin protective layer prepared from synthetic polyelectrolytes using layer‐by‐layer methodology were undertaken …”

Encapsulation of Pancreatic Islets Within Nano-Thin Functional Polyethylene Glycol Coatings for Enhanced Insulin Secretion

“…14,15 The large size of encapsulated islets along with the deterioration of the functionality of implanted islets in response to glucose lead researchers to consider functional microcapsules with insulinotropic agents for the purpose of improving the insulin secretion capability of encapsulated islets. [16][17][18][19][20] Even though the idea of encapsulating islets within functional coatings is promising, this technique, based on droplet generation by extrusion through a needle, produces capsules of constant outer diameters within the range of 400-800 mm.…”

Polyelectrolyte membrane scaffold sustains growth of neuronal cells

“…The PEI/PLL construction has been applied as a conformation ensuring suitable conditions for the growth of different cell populations . It is notable that the membrane material PEI/PLL was demonstrated to possess properties blocking bacterial cell transport .…”

“…The calculated average adhesion work occurring between layers was as follows Design of a membrane with a positively charged potential Zeta in the basal layer, with moderate adhesion between layers The PEI/PLL construction has been applied as a conformation ensuring suitable conditions for the growth of different cell populations. 29 It is notable that the membrane material PEI/PLL was demonstrated to possess properties blocking bacterial cell transport. 30 The build-up of PEI/PLL multilayers involves mainly hydrogen interactions between layers, thereby building the scaffold with a mildly positive potential in the basal layer (+14.93 AE 3.07 mV), whereas the external layer does not have an altered potential value (+14.11 AE 2.36 mV).…”