Chitosan‐<scp>B</scp>ased Nanocoatings for Hypothermic Storage of Living Cells

Bulwan, Maria; Antosiak-Iwańska, Magdalena; Godlewska, Ewa; Granicka, Ludomira; Zapotoczny, Szczepan; Nowakowska, Maria

doi:10.1002/mabi.201300258

Cited by 8 publications

(4 citation statements)

References 43 publications

(59 reference statements)

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“…For example, cationic polymers have been applied as gene carriers in nonviral gene therapy [ 48 ] and as drug delivery systems [ 49 ]; their potential use as anticancer drugs has also been suggested [ 50 ]. Moreover, a hydrogel containing GTMAC-modified polysaccharide can be applied to cell encapsulation and protein delivery [ 51 , 52 ]. Based on our results we can claim that high molecular weight linear polymers perform better as therapeutics than the cyclic or low molecular weight cationic polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Nonclinical Evaluation of Novel Cationically Modified Polysaccharide Antidotes for Unfractionated Heparin

et al. 2015

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Protamine, the only registered antidote of unfractionated heparin (UFH), may produce a number of adverse effects, such as anaphylactic shock or serious hypotension. We aimed to develop an alternative UFH antidote as efficient as protamine, but safer and easier to produce. As a starting material, we have chosen generally non-toxic, biocompatible, widely available, inexpensive, and easy to functionalize polysaccharides. Our approach was to synthesize, purify and characterize cationic derivatives of dextran, hydroxypropylcellulose, pullulan and γ-cyclodextrin, then to screen them for potential heparin-reversal activity using an in vitro assay and finally examine efficacy and safety of the most active polymers in Wistar rat and BALB/c mouse models of experimentally induced arterial and venous thrombosis. Efficacy studies included the measurement of thrombus formation, activated partial thromboplastin time, bleeding time, and anti-factor Xa activity; safety studies included the measurement of hemodynamic, hematologic and immunologic parameters. Linear, high molecular weight dextran substituted with glycidyltrimethylammonium chloride groups at a ratio of 0.65 per glucose unit (Dex40-GTMAC3) is the most potent and the safest UFH inhibitor showing activity comparable to that of protamine while possessing lower immunogenicity. Cationic polysaccharides of various structures neutralize UFH. Dex40-GTMAC3 is a promising and potentially better UFH antidote than protamine.

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Section: Discussionmentioning

confidence: 99%

Nonclinical Evaluation of Novel Cationically Modified Polysaccharide Antidotes for Unfractionated Heparin

et al. 2015

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“…14 In brief, aliquots of 10 islets (free or encapsulated) per well in triplicate 96-well plates were incubated in culture medium. We added 5 g/L MTT solution to culture medium at 1:10 dilution, which was followed by 2-hour incubation.…”

Section: Mitochondrial Activity Assaymentioning

confidence: 99%

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Kinasiewicz

Antosiak-Iwańska²,

Godlewska³

et al. 2018

Exp Clin Transplant

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Objectives: Immunoisolation of pancreatic islets of Langerhans performed by the encapsulation process may be a method to avoid immunosuppressive therapy after transplant. The main problem related to islet transplant is shortage of human pancreata. Resolution of this obstacle may be cryopreservation of encapsulated islets, which enables collection of sufficient numbers of isolated islets required for transplant and long-term storage. Here, we assessed the ability of encapsulated islets to function after longterm banking at low temperature. Materials and Methods: Islets of Langerhans isolated from rat, pig, and human pancreata were encapsulated within alginate-poly-L-lysine-alginate microcapsules. Cryopreservation was carried out using a controlled method of freezing (Kriomedpol freezer; Kriomedpol, Warsaw, Poland), and samples were stored in liquid nitrogen. After 10 years, the samples were thawed with the rapid method (with 0.75 M of sucrose) and then cultured. Results: We observed that microcapsules containing islets maintained their shape and integrity after thawing. During culture, free islets were defragmented into single cells, whereas encapsulated islets were still round in shape and compact. After 1, 4, and 7 days of culture of encapsulated islets, the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests showed increased mitochondrial activity. After they were thawed, the insulin secretion capacity was comparable with that obtained with fresh islets.Conclusions: Cryopreservation and storage of free and microencapsulated islets were possible for about 10 years, although only encapsulated islets retained viability and secretory properties.

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“…The commercial and laboratory chitosan products are not completely deacetylated and tend to be copolymers of N-acetyl glucosamine (NAG) and N-glucosamine [3][4][5] . The ratio of these two repeating units depends upon the source and method of preparation of chitosan but glucosamine unit predominates 6,7 .…”

Section: Introductionmentioning

confidence: 99%

Bioactive Hybrid Composite Membrane with Enhanced Antimicrobial Properties for Biomedical Applications

Davis

Kandasubramanian

2016

Def. Sc. Jl.

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Azadirachta indica extract has been explored as an antibiotic in hygienic chitosan matrix system to enhance antimicrobial and medicinal property in a cost effective manner. The hygienic composite system has been successfully fabricated via solvent casting. The antibacterial activities of the hybrid system were examined by agar diffusion method against gram positive S.aureus and gram negative K. pneumoniae. From conventional antibacterial test for 24 h, the system exhibited an excellent antimicrobial activity against both bacterial strains in ranges of 1.2 cm -1.5 cm for S. aureus and 1.8 cm -2.3 cm for K. pneumoniae Fourier transform infrared spectroscopy revealed successfully embedded A. indica on the chitosan substrate via weak electrostatic interaction, resulting in the easy release of the additive. Moreover, atomic force microscopy showed a membrane roughness of 0.084 nm which confirms the uniform distribution of the additive throughout the membrane. These hybrid membranes have potential applications in skin tissue engineering, wound healing and as coatings for implantable scaffold material.

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Chitosan‐Based Nanocoatings for Hypothermic Storage of Living Cells

Cited by 8 publications

References 43 publications

Nonclinical Evaluation of Novel Cationically Modified Polysaccharide Antidotes for Unfractionated Heparin

Nonclinical Evaluation of Novel Cationically Modified Polysaccharide Antidotes for Unfractionated Heparin

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Bioactive Hybrid Composite Membrane with Enhanced Antimicrobial Properties for Biomedical Applications

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