The experimental substantiation of the shelf-life of “Candidocyde” vaccine

Abstract

The experimental substantiation of the shelf-life of "Candidocyde" vaccine Because of the difficult situation with the diagnosis and therapy of candidiasis many researchers propose to use vaccines as an alternative to anti-fungal drugs for preventing and treating candidal infections. The authors have developed "Candidocyde" vaccine against candidiasis. Aim. To determine experimentally the shelf-life of the solution of "Candidocyde" vaccine. Materials and methods. "Candidocyde" vaccine was stored at two temperature regimes: in the refrigerator at the temperature of 2-8 °С and at the room temperature of (15-25 °С). Antibody titers of the solution of "Candidocyde" vaccine were assessed every three months to prevent and treat candidiasis. For this purpose the reagent kit for immunoassay detection of antibodies of class G to C. albicans using the test system ELISA "Vector-best" was applied. Results and discussion. When conducting the studies of the vaccine stored in glass vials protected from light at the temperature from 2 ºС to 8 ºС it was found that the activity of the solution was preserved for 2 years and 3 months when preventing and treating candidal infections. The titers of antibodies in animals remained at the level of 1 : 600-1 : 4000 when preventing and treating candidiasis. The positive results were recorded in all animals in the group, i.e. all animals were healthy. It indicates that the solution of "Candidocyde" vaccine is stable for 2 years. Conclusions. When studying the shelf-life of the solution of "Candidocyde" vaccine based on antigens of cells of C. albicans and C. tropicalis fungi it has been found that it is stable for 2 years of storage in glass vials protected from light at the temperature of 2-8 ºС.

Analytical and Statistical Study of the Range of Antifungal Medicines of Local Action

“…Therefore, to effectively combat fungal infections, it is advisable to conduct a marketing analysis of antifungal agents for local use, which are presented in the pharmaceutical market of Ukraine, to determine the availability of convenient dosage forms and expand production of new drugs not only synthetic but also based on plant raw materials.From year to year in the world there is a rapid increase in the number of cases of fungal diseases of the skin and its appendages. According to WHO statistics, every third person on our planet is diagnosed with mycosis [1], and the incidence of fungal infections increases with age 2.5 times every 10 years [5]. This is due to adverse environmental conditions, reduced immune systems, long-term and not always rational use of broad-spectrum antibacterial agents, glucocorticosteroids, cytostatics and contraceptives.…”

“…From year to year in the world there is a rapid increase in the number of cases of fungal diseases of the skin and its appendages. According to WHO statistics, every third person on our planet is diagnosed with mycosis [1], and the incidence of fungal infections increases with age 2.5 times every 10 years [5]. This is due to adverse environmental conditions, reduced immune systems, long-term and not always rational use of broad-spectrum antibacterial agents, glucocorticosteroids, cytostatics and contraceptives.…”