The expression and distribution of deoxyribonucleic acid repair and apoptosis markers in testicular germ cells of infertile varicocele patients resembles that of old fertile men

El‐Domyati, Moetaz; Al-Din, Abo-Bakr M.; Barakat, Manal; El-Fakahany, Hasan; Honig, Stanton C.; Xu, Jianbing; Sakkas, Denny

doi:10.1016/j.fertnstert.2008.10.031

Cited by 25 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oxidative stress triggers apoptosis, which is considered one of the mechanisms that contribute to the pathophysiology of varicocele [44]. As a matter of fact, apoptotic activation was also observed in germ cells, in testicular tissues, and in ejaculated spermatozoa [45]; this impaired condition led us to hypothesize the presence of damaged DNA in sperm [46].…”

Section: Discussionmentioning

confidence: 95%

Nutraceutical Effects of Lycopene in Experimental Varicocele: An “In Vivo” Model to Study Male Infertility

et al. 2020

View full text Add to dashboard Cite

Varicocele is one of the main causes of infertility in men. Oxidative stress and consequently apoptosis activation contribute to varicocele pathogenesis, worsening its prognosis. Natural products, such as lycopene, showed antioxidant and anti-inflammatory effects in several experimental models, also in testes. In this study we investigated lycopene effects in an experimental model of varicocele. Male rats (n = 14) underwent sham operations and were administered with vehicle (n = 7) or with lycopene (n = 7; 1 mg/kg i.p., daily). Another group of animals (n = 14) underwent surgical varicocele. After 28 days, the sham and 7 varicocele animals were euthanized, and both operated and contralateral testes were weighted and processed. The remaining rats were treated with lycopene (1 mg/kg i.p., daily) for 30 days. Varicocele rats showed reduced testosterone levels, testes weight, Bcl-2 mRNA expression, changes in testes structure and increased malondialdehyde levels and BAX gene expression. TUNEL (Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling) assay showed an increased number of apoptotic cells. Treatment with lycopene significantly increased testosterone levels, testes weight, and Bcl-2 mRNA expression, improved tubular structure and decreased malondialdehyde levels, BAX mRNA expression and TUNEL-positive cells. The present results show that lycopene exerts beneficial effects in testes, and suggest that supplementation with the tomato-derived carotenoid might be considered a novel nutraceutical strategy for the treatment of varicocele and male infertility.

show abstract

Section: Discussionmentioning

confidence: 95%

Nutraceutical Effects of Lycopene in Experimental Varicocele: An “In Vivo” Model to Study Male Infertility

et al. 2020

View full text Add to dashboard Cite

show abstract

“…PARP, a downstream target of caspase-3, can be cleaved in fragments of 89 and 24 kDa by an active form of caspase-3. This cleaved PARP loses the ability to participate in DNA repair, which results in apoptosis and is a useful hallmark of this type of cell death [ 44 , 45 ]. Therefore, inhibiting extensive activation of caspase-3 and PARP may be the key to decreasing Leydig cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Zhou

Zhang

et al. 2016

Mediators of Inflammation

View full text Add to dashboard Cite

This study was carried out to investigate whether ADM can modulate LPS-induced inflammation and apoptosis in rat Leydig cells. Leydig cells were treated with ADM before LPS-induced cytotoxicity. We determined the concentrations of ROS, MDA, GSH, LDH, and testosterone and the MMP. The mRNA levels of IL-1, IL-6, iNOS, and COX-2 were obtained, and the concentrations of IL-1, IL-6, NO, and PGE2 were determined. Apoptosis was assessed by TUNEL and detection of DNA fragmentation. The levels of mRNA and protein were determined for Bcl-2, Bax, caspase-3, and PARP. The protein contents for total and p-Akt were measured. ADM pretreatment significantly elevated the MMP and testosterone concentration and reduced the levels of ROS, MDA, GSH, and LDH. ADM pretreatment significantly decreased the mRNA levels of IL-1, IL-6, iNOS, and COX-2 and the concentrations of IL-1, IL-6, NO, and PGE2. LPS-induced TUNEL-positive Leydig cells were significantly decreased by ADM pretreatment, a result further confirmed by decreased DNA fragmentation. ADM pretreatment decreased apoptosis by significantly promoting Bcl-2 and inhibiting Bax, caspase-3, and PARP expressions. The LPS activity that reduced p-Akt level was significantly inhibited by ADM pretreatment. ADM protected rat Leydig cells from LPS-induced inflammation and apoptosis, which might be associated with PI3K/Akt mitochondrial signaling pathway.

show abstract

“…Chang et al (33) suggested that certain protein-development apoptotic mechanisms that are increased in varicocele-associated cases might originate in the cytoplasmic droplet or mitochondria of spermatocytes and then might function within the nucleus of the cell. El-Domyati et al (34) added that significant differences in DNA damage repair-associated proteins and apoptosis markers were observed in infertile men with varicocele compared with fertile young men. Old fertile men showed similar expression of the same markers compared with infertile varicocele patients.…”

Section: Discussionmentioning

confidence: 99%

Sperm caspase-9 in oligoasthenoteratozoospermic men with and without varicocele

Zalata

El-Mogy

Abdel-Khabir

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

The expression and distribution of deoxyribonucleic acid repair and apoptosis markers in testicular germ cells of infertile varicocele patients resembles that of old fertile men

Cited by 25 publications

References 31 publications

Nutraceutical Effects of Lycopene in Experimental Varicocele: An “In Vivo” Model to Study Male Infertility

Nutraceutical Effects of Lycopene in Experimental Varicocele: An “In Vivo” Model to Study Male Infertility

Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Sperm caspase-9 in oligoasthenoteratozoospermic men with and without varicocele

Contact Info

Product

Resources

About