The expression and functional characterization associated with cell apoptosis and proteomic analysis of the novel gene MLAA-34 in U937 cells

Zhang, Wenjuan; Zhang, Wanggang; Zhang, Pengyu; Cao, Xing‐Mei; He, Aili; Chen, Yinxia; Gu, Liufang

doi:10.3892/or.2012.2129

Cited by 7 publications

(21 citation statements)

References 38 publications

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“…This gene is homologous to the known human CAB39L gene and has been confirmed to be a novel splice variant of CAB39L (5,9). The authors of the present study previously reported that MLAA-34 may act as an anti-apoptosis factor in vitro via interacting with Ras, Wnt or calcium and chemokine signaling pathways, and lentivirus-mediated ectopic expression of MLAA-34 in U937 cells markedly suppressed the spontaneous apoptosis of U937 cells (5,12). Additional clinical studies uncovered that high MLAA-34 expression levels usually indicated unfavorable clinical features of patients with AML, and may be used as an early biomarker for detection of relapse (5,9,12).…”

Section: Discussionmentioning

confidence: 54%

“…In conclusion, the evidence that the wild-type MLAA-34 is an anti-apoptotic factor (12,24) and that overexpression of MLAA-34 was observed in patients with AML with poor response to standard chemotherapy indicated that MLAA-34 is a candidate oncogene. Thus, the present study shed light on the diagnosis and treatment of AML, and MLAA-34 may be a novel marker for AML therapy.…”

Section: Discussionmentioning

confidence: 97%

“…AY288977.2) is one of the novel identified leukemia-associated antigens and a candidate oncogene (5,9). As a novel splice variant of calcium binding protein 39-like (CAB39L), MLAA-34 exclusively reacts with sera from patients with allogeneic leukemia but not with normal donor sera (5,8,11,12). In addition, MLAA-34 has been indicated to be a potent anti-apoptotic factor associated with carcinogenesis or progression of AML (5).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, MLAA-34 has been indicated to be a potent anti-apoptotic factor associated with carcinogenesis or progression of AML (5). Downregulation of MLAA-34 expression significantly suppresses the proliferation and increases the spontaneous apoptosis of U937 cells in vitro (5,12). Additional studies uncovered that MLAA-34 may be involved in cell apoptosis through interaction with the Ras, Wnt, calcium and chemokine signaling pathways in U937 cells (5,9,12).…”

Section: Introductionmentioning

confidence: 99%

“…Downregulation of MLAA-34 expression significantly suppresses the proliferation and increases the spontaneous apoptosis of U937 cells in vitro (5,12). Additional studies uncovered that MLAA-34 may be involved in cell apoptosis through interaction with the Ras, Wnt, calcium and chemokine signaling pathways in U937 cells (5,9,12). It has been indicated that 13 of the annotated proteins (PGK1, GAPDH, CRMP1, TBK-1, SEPT7, CLTC, PPP2CA, SOD2, PARK7, HSPA9, TXN, ESR1 and YWHAE) may interact with MLAA-34 and be directly involved in carcinogenesis (12).…”

Section: Introductionmentioning

confidence: 99%

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C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

Lei

Chen

et al. 2017

Oncology Letters

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Monocytic leukemia-associated antigen-34 (MLAA-34) is a novel monocytic leukemia-associated antigen and a candidate oncogene. The aim of the present study was to investigate the involvement of the MLAA-34 gene in acute myeloid leukemia (AML). MLAA-34 expression level, chromosome location, gene copy number and single nucleotide polymorphisms (SNPs) of 40 patients with AML and 5 healthy volunteers were analyzed by reverse transcription-polymerase chain reaction, fluorescence in situ hybridization and DNA sequencing. The effects of MLAA-34 mutation on overall survival (OS) and progression-free survival (PFS) of patients with AML were also analyzed. MLAA-34 was significantly upregulated in patients with AML when compared with volunteer controls, and this upregulation was associated with a C59T SNP site located in the second exon of MLAA-34. MLAA-34 was mapped to 13q14.2 and no translocation was observed in patients with AML. In addition, this SNP site is affinitive to the well-known molecular markers of AML, including Fms-like tyrosine kinase 3 and DNA methyltransferase 3A, as well as extramedullary lesions, periphery leukocyte numbers, remission and cytogenetic abnormalities of patients with AML. Patients with AML with MLAA-34 C59T mutations had significantly shorter OS and PFS times compared with that of patients without C59T mutations. The present findings indicated that the MLAA-34 C59T mutation was a high-risk factor for recurrence of AML, and may be a candidate target for AML therapy.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

Lei

Chen

et al. 2017

Oncology Letters

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Bioinformatic prediction and functional characterization of human KIAA0100 gene

Lan

et al. 2017

Journal of Pharmaceutical Analysis

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Our previous study demonstrated that human KIAA0100 gene was a novel acute monocytic leukemia-associated antigen (MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown to date. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using online softwares; Secondly, Human KIAA0100 gene expression was downregulated by the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system in U937 cells. Cell proliferation and apoptosis were next evaluated in KIAA0100-knockdown U937 cells. The bioinformatic prediction showed that human KIAA0100 gene was located on 17q11.2, and human KIAA0100 protein was located in the secretory pathway. Besides, human KIAA0100 protein contained a signalpeptide, a transmembrane region, three types of secondary structures (alpha helix, extended strand, and random coil) , and four domains from mitochondrial protein 27 (FMP27). The observation on functional characterization of human KIAA0100 gene revealed that its downregulation inhibited cell proliferation, and promoted cell apoptosis in U937 cells. To summarize, these results suggest human KIAA0100 gene possibly comes within mitochondrial genome; moreover, it is a novel anti-apoptotic factor related to carcinogenesis or progression in acute monocytic leukemia, and may be a potential target for immunotherapy against acute monocytic leukemia.

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Generation and characterization of U937-TR: a platform cell line for inducible gene expression in human macrophages

Galindo

Clavijo-Ramírez

2020

Parasitology

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Monocytes and macrophages are involved in a wide range of biological processes and parasitic diseases. The characterization of the molecular mechanisms governing such processes usually requires precise control of the expression of genes of interest. We implemented a tetracycline-controlled gene expression system in the U937 cell line, one of the most used in vitro models for the research of human monocytes and macrophages. Here we characterized U937-derived cell lines in terms of phenotypic (morphology and marker expression) and functional (capacity for phagocytosis and for Leishmania parasite hosting) changes induced by phorbol-12-myristate-13-acetate (PMA). Finally, we provide evidence of tetracycline-inducible and reversible Lamin-A gene silencing of the PMA-differentiated U937-derived cells.

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The expression and functional characterization associated with cell apoptosis and proteomic analysis of the novel gene MLAA-34 in U937 cells

Cited by 7 publications

References 38 publications

C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

C59T mutation in exon 2 of monocytic leukemia-associated antigen-34 gene indicates a high risk of recurrence of acute myeloid leukemia

Bioinformatic prediction and functional characterization of human KIAA0100 gene

Generation and characterization of U937-TR: a platform cell line for inducible gene expression in human macrophages

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