The expression of class I patatin gene fusions in transgenic potato varies with both gene and cultivar

Blundy, Keith S.; Blundy, Margaret; Carter, D.; Wilson, Frank J.; Park, W. D.; Burrell, M. M.

doi:10.1007/bf00017925

Cited by 41 publications

(25 citation statements)

References 31 publications

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“…First, the FEYgene may be normally subject to tight regulation and the abnormal position of the introduced genomic fragment could result in abnormal regulation of FEY. Positional effects on introduced genes have been reported often (Blundy et al, 1991;Dean et al, 1988;Peach and Velten, 1991). Alternatively, although the fey mutant has been backcrossed two times, it is still possible that a second, tightly linked mutation is present.…”

Section: Discussionmentioning

confidence: 99%

The forever young gene encodes an oxidoreductase required for proper development of the Arabidopsis vegetative shoot apex

Callos

Dirado

et al. 1994

The Plant Journal

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SummaryIn plant development, leaf primordia are formed on the flanks of the shoot apical meristem in a highly predictable pattern. The cells that give rise to a primordium are sequestered from the apical meristem. Maintenance of the meristem requires that these cells be replaced by the addition of new cells. Despite the central role of these activities in development, the mechanism controlling and coordinating them is poorly understood. These processes have been characterized in the Arabidopsis mutant forever young(fey). The fey mutation results in a disruption of leaf positioning and meristem maintenance. The predicted FEY protein shams significant homology to a nodulin and limited homology to various reductases, it is proposed that FEY plays a role in communication in the shoot apex through the modification of a factor regulating meristem development.

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Section: Discussionmentioning

confidence: 99%

The forever young gene encodes an oxidoreductase required for proper development of the Arabidopsis vegetative shoot apex

Callos

Dirado

et al. 1994

The Plant Journal

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“…The gene was deleted at the 5' end to 20 bp from the translation start site and 50 bp from the 3' end of the coding sequence with the exonuclease BAL 31. The result was then ligated into the PS3-27 GUS plasmid as described by Blundy et al (1991) after removal of the fl-glucuronidase (GUS) coding sequence with the restriction endonucleases Smal and SstI. The resulting plasmids, containing either GUS or PFK(ATP) attached to a tuberspecific patatin promoter, were then transferred from E. coli to Agrobacterium tumefaciens by triparental mating.…”

Section: Methodsmentioning

confidence: 99%

Genetic manipulation of 6-phosphofructokinase in potato tubers

Burrell¹,

Mooney²,

Blundy³

et al. 1994

Planta

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Abstract. The aim of this work was to discover whether genetic manipulation of 6-phosphofructokinase [EC 2.7.1.11; PFK(ATP)] influenced the rate of respiration of tuber tissue of Solanum tuberosum L. Transgenic plants were produced that contained the coding sequence of the Escherichia coli pJkA gene linked to a patatin promoter. Expression of this chimaeric gene in tubers resulted in a 14-to 21-fold increase in the maximum catalytic activity of PFK(ATP) without affecting the activities of the other glycolytic enzymes. Tubers, and 'aged' disks of tuber tissue, from transformed plants showed no more than a 30% fall in the content of hexose 6-monophosphates; the other intermediates of glycolysis increased three-to eightfold. Fructose-2,6-bisphosphate was barely detectable in aged disks of transformed tubers. The relative rates of/4CO2 production from [1-14C] -and [6-14C]-glucose supplied to disks of transformed and control tubers were similar. Oxygen uptake and CO 2 production by aged disks of transformed tubers did not differ significantly from those from control tubers. The same was true Of CO2 production, in air, and in nitrogen, for tuber tissue. It is concluded that PFK(ATP) does not dominate the control of respiration in potato tubers.

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“…In addition, different potato genotypes, even among common cultivars, have significant variation in quantity and isoforms under altered developmental stages and organs, and also under different physiological and environmental conditions. [33][34][35] Patatin variation occurs with germplasm enhancement practice especially with using wild relatives. Furthermore, expression of other potato tuber proteins has been reported to change in response to environmental factors, such as abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Immunoproteomic and Two-Dimensional Difference Gel Electrophoresis Analysis of Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)-Transgenic Potato

Nakamura¹,

Satoh²,

Nakamura³

et al. 2010

Biological & Pharmaceutical Bulletin

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To increase the quality and yield of crops, transgenic plants have been developed that show enhanced resistance to insects or herbicides, or improved growth and nutrient status. In addition, transgenic plants expressing proteins associated with stress responses are being developed in an effort to produce crops with greater resistance to stresses such as cold, heat, and salt.1) Genetic engineering of a transcriptional factor may affect expressions of multiple genes, therefore, there may be unintended effects on protein expression levels in transgenic plants. For appropriate safety assessment, therefore, it is important to evaluate the proteomics of transgenic plants, particularly with respect to allergenicity.Even after the establishment of the 2003 Codex guidelines for methods to assess safety of foods derived from transgenic organisms, there is still controversy regarding methods used to test their allergenicity.2) Allergenicity of foods derived from transgenic organisms has been evaluated using immunological methods such as immunoblots, histamine release assays, skin prick tests, and oral exposure. 3,4) Immunoproteomic analysis of allergens with sera from allergic patients is a useful method for comprehensively comparing unknown allergens as well as known allergens in extracts of foods derived from transgenic organisms. Previously, we reported application of the immunoproteome to assess allergenicities of GH-1 (growth hormone 1)-transgenic amago salmon 5) and enhanced green fluorescent protein (EGFP)-transgenic chicken meat.6) The two-dimensional difference gel electrophoresis (2D-DIGE) method, a gel-based proteomic technology, is useful for evaluating relative differences in protein expression, if any, between non-transgenic and transgenic crops. The CyDye DIGE fluors used to label samples are spectrally resolvable, size-and charge-matched fluorescent dyes. 7,8) The method enables analysis of one or more samples on the same gel and is highly sensitive, and has a wide range of detection.Potato (Solanum tuberosum) is a staple food that is consumed worldwide. Various transgenic potatoes have been developed and their food safety has been assessed using serological and proteomic techniques.9-11) Several cases of potato allergies have been reported, 12-16) and a major potato allergen that causes hypersensitive reactions has been identified as patatin (Sola t 1).17) Potato allergy is considered to be cross-reactive with latex or pollens, and their cross-reactive fruit and vegetables. [18][19][20] Other potato allergens, cathepsin D-, cysteine-, and serine protease inhibitors, all of which are members of the trypsin inhibitor family, have been designated as Sola t 2, Sola t 3, and Sola t 4, respectively. 21) These allergens make up approximately 70-80% of total soluble potato proteins. 22)In this study, we investigated the allergenicity of stresstolerant transgenic potatoes 23,24) To produce crops that are more tolerant to stresses such as heat, cold, and salt, transgenic plants have been produced those express stre...

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The expression of class I patatin gene fusions in transgenic potato varies with both gene and cultivar

Cited by 41 publications

References 31 publications

The forever young gene encodes an oxidoreductase required for proper development of the Arabidopsis vegetative shoot apex

The forever young gene encodes an oxidoreductase required for proper development of the Arabidopsis vegetative shoot apex

Genetic manipulation of 6-phosphofructokinase in potato tubers

Immunoproteomic and Two-Dimensional Difference Gel Electrophoresis Analysis of Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)-Transgenic Potato

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