The expression of Clostridium perfringens consensus beta2 toxin is associated with bovine enterotoxaemia syndrome

Lebrun, Marielle; Filée, Patrice; Mousset, Bénédicte; Desmecht, Daniël; Galleni, Moreno; Mainil, Jacques; Lindén, Annick

doi:10.1016/j.vetmic.2006.10.020

Cited by 48 publications

(35 citation statements)

References 16 publications

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“…However, the role of beta2 toxin in SNE might be variant dependent since all cpb2-carrying C. perfringens isolates from SNE birds carried the atypical allele, and the cpb2-carrying C. perfringens isolate from the SPF bird carried the consensus three allele. The role of the variants of beta2 toxin in intestinal disorders in various animal species is still under discussion (Jost et al, 2005;Lebrun et al, 2007;van Asten et al, 2008). The difference in expression of cpb2 between the various atypical cpb2 allele-harbouring C. perfringens isolates from the same bird is in line with what has been demonstrated in another study (Jost et al, 2005).…”

Section: Discussionsupporting

confidence: 76%

“…Beta2 toxin, encoded by cpb2, which is another toxin produced by many C. perfringens isolates (Gibert et al, 1997), has been associated with NE in horses (Bacciarini et al, 2003), ruminants (Lebrun et al, 2007), and pigs Waters et al, 2003). However, it has been suggested that beta2 toxin is not involved in NE in poultry (Gholamiandekhordi et al, 2006;Crespo et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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NetB-producing and beta2-producingClostridium perfringensassociated with subclinical necrotic enteritis in laying hens in the Netherlands

et al. 2012

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Since 2006 increasing numbers of laying hen flocks with decreased production have been reported in the Netherlands. At necropsy, birds from affected flocks showed multifocal areas of necrosis in the duodenum. Histologically the duodenum had moderate to marked villus atrophy and fusion with crypt hyperplasia and a mixed inflammatory infiltrate within the lamina propria underlying focal areas of degenerative epithelium. Multifocally, free within the intestinal lumen and associated with epithelial necrosis, were marked numbers of large rod-shaped bacteria. Anaerobic culturing and subsequent toxin typing revealed, in 19 out of 73 affected birds, the presence of Clostridium perfringens strains, either type A or type C harbouring the atypical allele of cpb2 and netB. Eighteen out of these 19 birds carried C. perfringens strains capable of producing beta2 toxin in vitro and all of these birds harboured C. perfringens strains capable of producing NetB toxin in vitro. In contrast, specific pathogen free (SPF) birds lacked gross or histological lesions in their duodenum, and C. perfringens type C was isolated from four out of 15 SPF birds tested. One of these isolates harboured the consensus three allele of cpb2 that produced beta2 toxin in vitro. None of the C. perfringens isolates originating from SPF birds harboured netB. These findings might indicate that the NetB toxin produced by C. perfringens is associated with subclinical necrotic enteritis in layers, whereas the involvement of beta2 toxin in subclinical necrotic enteritis, if any, might be variant dependent.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

NetB-producing and beta2-producingClostridium perfringensassociated with subclinical necrotic enteritis in laying hens in the Netherlands

et al. 2012

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“…Similares incapacidades han sido observadas en inoculaciones con cultivos de C. perfringens tipo A cpb2 +vo en yeyuno de vacunos (Ewoldt y Anderson, 2005). Esta incapacidad enterotóxica tal vez responda a que la toxina â2 no habría sido sintetizada en los aislados, pues la expresión de gen cpb2 es irregular y dependiente del medio de cultivo empleado (medios específicos suplementados con antibióticos) (Vilei et al, 2005) o del tipo de alelo cpb2 involucrado, que casi siempre se expresa en su forma consensus o pocas veces en lo atípico (Lebrun et al, 2007).…”

Section: Resultados Y Discusiónunclassified

ANÁLISIS In vitro e in vivo DE SOBREDONANTES CRUDOS DE Clostridium perfringens AISLADOS DE CASOS DE ENTEROTOXEMIA EN ALPACAS.

D.³

et al. 2012

Rev. investig. vet. Perú

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“…there are other reports that relate the pathogenicity of cpb2 expressing C. perfringens with clinical diarrhea in neonates, where piglets showed severe diarrhea, which indicates the fact that β2 toxin plays a larger role in neonatal diarrhea (WaterS et al, 2003). There are reports of β2 toxin producing C. perfringens associated with caprine enterotoxaemia (DraY, 2004; in subclinical necrotic enteritis in laying hens (aLLaart et al, 2012), hemorrhagic bowel syndrome (hUS) or enterotoxaemia in bovines (DeNNISON et al, 2005;LeBrUN et al, 2007;FerrareZI et al, 2008) and being involved in typhlocolitis or enterocolitis in equines (VILeI et al, 2005;tIMONeY et al, 2005) C. perfringens type D, that harbours the genes cpa (α toxin) and etx (ε toxin), was isolated from 9 samples during our survey. In previous instances, the association has been already reported of C. perfringens type D with clinical cases of enterotoxemia in goats (FerNaNDeZ-MIYakaWa and UZaL, 2003;GreCO et al, 2005;MIYaShIrO et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Clostridium perfringens toxinotypes, Enteropathogenic Escherichia coli, rotavirus and coronavirus in diarrheic fecal samples of neonatal goat kids

et al. 2018

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In the present study, out of 1156 neonatal goat kids, 238 showing clinical diarrhea were used for detection of toxinotypes of Clostridium perfringens, enteropathogenic E. coli (ePeC), Group a rotavirus (GarV) and Bovine coronavirus (BCV). Isolation and toxinotyping of isolates were done by multiplex Polymerase chain reaction (PCr) using primers for cpa, cpb, cpb2, etx and iap genes. For EPEC, isolation and identification were done using bfpa gene and SYBr green based real time PCr (qPCr). GarV and BCV were detected, by onestep rt-PCr (osrt-PCr). the incidence of C. perfringens was 15.13% with 75% isolates toxinotype a, 25% type D and 61.11% of isolates carrying the β2-toxin gene. The incidence of EPEC was 68.07% based on qPCr, whereas 21.85% were positive for GarV and 15.97% for BCV by osrt-PCr. there was mixed infection of C. perfringens and ePeC in 11.76% and 3.78% for C. perfringens and GarV and 2.1% of C. perfringens and BCV. ePeC and GarV was 19.74% and ePeC plus BCV positivity was 11.34%. GarV and BCV was 5.88%, and 4.20% had mixed infection of ePeC, GarV and BCV. Of the total diarrheic kids sampled, 0.84% had mixed infection of C. perfringens, GARV, BCV and EPEC. On the basis of the above findings, it may be concluded that isolation, multiplex PCr and real time PCr facilitated the characterization of circulating C. perfringens toxinotypes and ePeC in goats reared under semi-arid conditions. the importance of enteritis caused by GarV and BCV and their role in mixed infection in goats requires extensive screening and pathogenicity studies to associate the symptoms with disease.

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The expression of Clostridium perfringens consensus beta2 toxin is associated with bovine enterotoxaemia syndrome

Cited by 48 publications

References 16 publications

NetB-producing and beta2-producingClostridium perfringensassociated with subclinical necrotic enteritis in laying hens in the Netherlands

NetB-producing and beta2-producingClostridium perfringensassociated with subclinical necrotic enteritis in laying hens in the Netherlands

ANÁLISIS In vitro e in vivo DE SOBREDONANTES CRUDOS DE Clostridium perfringens AISLADOS DE CASOS DE ENTEROTOXEMIA EN ALPACAS.

Molecular detection of Clostridium perfringens toxinotypes, Enteropathogenic Escherichia coli, rotavirus and coronavirus in diarrheic fecal samples of neonatal goat kids

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