The expression of molecular mediators in the idiopathic cutaneous calcification and ossification

Kim, So Young; Choi, Hae Young; Myung, Ki Bum; Choi, You Won

doi:10.1111/j.1600-0560.2007.00904.x

Cited by 34 publications

(26 citation statements)

References 27 publications

(50 reference statements)

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“…Regardless of etiology, the pathogenesis of HO is multifactorial and requires the interaction of several biologic mediators and cell types. Efforts to identify the molecular mechanisms associated with its development have focused on the conditions permissive to osteogenesis in the cellular microenvironment [10,15,29]. An understanding of these mechanisms could lead to better means of risk stratification and prophylaxis.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic Gene Expression Correlates With Development of Heterotopic Ossification in War Wounds

Evans

Potter

Brown

et al. 2013

Clin Orthop Relat Res

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Background Heterotopic ossification (HO) is a frequent complication of modern wartime extremity injuries. The biological mechanisms responsible for the development of HO in traumatic wounds remain elusive. Question/purposes The aims of our study were to (1) characterize the expression profile of osteogenesis-related gene transcripts in traumatic war wounds in which HO developed; and (2) determine whether expression at the mRNA level correlated with functional protein expression and HO formation. Methods Biopsy specimens from 54 high-energy penetrating extremity wounds obtained at the initial and final surgical débridements were evaluated. The levels of selected osteogenic-related gene transcripts from RNA extracts were assessed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. As a result of its key role in osteogenesis, the concentration of BMP-2 in the effluent of 29 wounds also was determined. Conclusions Important differences exist in the osteogenic gene expression profile of wounds in which HO developed compared with wounds in which it did not develop. The upregulation of multiple osteogenesis-related gene transcripts indicates the presence of a proosteogenic environment necessary for ectopic bone formation in traumatic wounds.

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Section: Discussionmentioning

confidence: 99%

Osteogenic Gene Expression Correlates With Development of Heterotopic Ossification in War Wounds

Evans

Potter

Brown

et al. 2013

Clin Orthop Relat Res

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“…OCN, OPN and ONN are abundant non-collagenous proteins of the normal bone matrix (10)(11)(22)(23). Collagen protein is well known as a major organic substance of bone matrix, and the remaining 10% are non-collagenous proteins, such as OCN, OPN, and ONN.…”

Section: Discussionmentioning

confidence: 99%

“…The maturation of osteoblasts can be determined to some extent by their expression of OCN, OPN and ONN (10)(11)(25)(26). OCN, the most abundant non-collagenous protein, is distributed into normal bone and has been shown by gene knockout technology to be a negative regulator of ossification (10). OCN, OPN, and ONN were used to clarify matrix formation.…”

Section: Discussionmentioning

confidence: 99%

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A histopathological and immunohistochemical study of pathological ossification in epulis osteoplastica

Tajima

2009

Oral Med Pathol

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Epulis osteoplastica shows pathological ossification, but details of the mecha nism involved are still unclear. In the present study we carried out histopathological and immunohistochemical analyses to clarify the potential for ossification in epulis osteoplastica (n = 8), in comparison with epulis fibrosa, using several markers. Epulis osteoplastica showed particulate, massive, trabecular and osteoid ossification surrounded by proliferating fibroblasts and fibrous tissues under the mucosal squamous epithelium. Epulis fibrosa showed proliferation of fibroblasts and collagen fibers without ossification. Although positive immunoreactivity for bone morpho genetic proteins 2, 4 and 6, osteocalcin, osteopontin, and ostenectin was observed in the fibroblasts of epulis osteoplastica, the cell positivity rates were much higher in epulis osteoplastica than in epulis fibrosa, suggesting that these substances were associated with pathological ossification in epulis osteoplastica. Furthermore, the findings indicated that the fibroblasts in epulis osteoplastica might have a higher ability to produce bone matrix production than those in epulis fibrosa.

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“…Overuse was also reported to upregulate the expression of cartilage-associated genes and downregulate the expression of tendon-associated genes in rat supraspinatous tendons [65] and in horse superficial digital flexor tendons [66]. Indeed, aberrant differentiation of stem cells is also seen in the pathogenesis of arterial calcification [67], skin calcification [68], skeletal calcification [69] and fracture nonunion [70].…”

Section: Evidence For Erroneous Differentiation Of Tdscs In the Pathomentioning

confidence: 92%

Tendon-Derived Stem Cells (TDSCs): From Basic Science to Potential Roles in Tendon Pathology and Tissue Engineering Applications

Lui

Chan

2011

Stem Cell Rev and Rep

142

119

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Traditionally, tendons are considered to only contain tenocytes that are responsible for the maintenance, repair and remodeling of tendons. Stem cells, which are termed tendon-derived stem cells (TDSCs), have recently been identified in tendons. This review aims to summarize the current information about the in vitro characteristics of TDSCs, including issues related to TDSC isolation and culture, their cell morphology, immunophenotypes, proliferation and differentiation characteristics and senescence during in vitro passaging. The challenges in studying the functions of these cells are also discussed. The niche where TDSCs resided essentially provides signals that are conducive to the maintenance of definitive stem cell properties of TDSCs. Yet the niche may also induce pathologies by imposing an aberrant function on TDSCs or other targets. The possible niche factors of TDSCs are herein discussed. We presented current evidences supporting the potential pathogenic role of TDSCs in the development of tendinopathy with reference to the recent findings on the altered biological responses of these cells in response to their potential niche factors. The use of resident stem cells may promote engraftment and differentiation of transplanted cells in tendon and tendon-bone junction repair because the tendon milieu is an ideal and familiar environment to the transplanted cells. Evidences are presented to show the potential advantages and results of using TDSCs as a new cell source for tendon and tendon-bone junction repair. Issues pertaining to the use of TDSCs for tissue repair are also discussed.

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The expression of molecular mediators in the idiopathic cutaneous calcification and ossification

Cited by 34 publications

References 27 publications

Osteogenic Gene Expression Correlates With Development of Heterotopic Ossification in War Wounds

Osteogenic Gene Expression Correlates With Development of Heterotopic Ossification in War Wounds

A histopathological and immunohistochemical study of pathological ossification in epulis osteoplastica

Tendon-Derived Stem Cells (TDSCs): From Basic Science to Potential Roles in Tendon Pathology and Tissue Engineering Applications

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