The expression of renin-angiotensin system components in the human gastric mucosa

Abstract

Introduction: The aim of the present study was to map the distribution of representative protein components of the renin-angiotensin system (RAS) in the human gastric mucosa. Materials and methods:Biopsies from the antral and corporal mucosa of healthy Helicobacter pylori negative and positive volunteers were assessed by histology, Western blot and immunohistochemistry for angiotensin II subtype 1 and 2 receptors (AT1R, AT2R) and other RAS components (angiotensinogen, renin, angiotensin converting enzyme, and neprilysin). Mucosal levels of myeloperoxidase (MPO) served as a protein marker of neutrophil infiltration.

A Modern Understanding of the Traditional and Nontraditional Biological Functions of Angiotensin-Converting Enzyme

“…Inflammation Stomach High expression of AT1 receptor in Helicobacter pylori (Hallersund et al, 2011). Greater expression of AT1 receptor in cancer cells than in normal tissue (Kinoshita et al, 2009).…”

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“…Sections were incubated with a rabbit polyclonal anti-AT1R antibody (1: 1000 dilution, sc-1173, RRID: AB_2305402, Santa Cruz Biotechnology Inc.; Santa Cruz, CA) and processed for immunohistochemistry using avidin-biotin-peroxidase complex with diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Previous studies have shown the AT1R antibody specificity, by performing peptide competition studies using the specific blocking peptide (sc-1173P) [32-34]. A good correlation was also demonstrated between Western blot using this antibody and the quantification of AT1R in tissue by radio-ligand binding with I125-labeled ANGII [32].…”

AGT A-20C (rs5050) gene polymorphism and ulcer occurrence in patients treated with low-dose aspirin: a case-control study / Polimorfismul AGT A-20C și ulcerele gastro-duodenale la pacienții sub tratament cu aspirină în doze antiagregante: studiu caz-control

“…AngII is a stress hormone that regulates vasoconstriction in resistant arteries, which can determine an impairment of gastric blood flow in stress conditions. On the other hand, the presence of higher expression of angiotensin type 1 receptors (AT1R) in H. pylori infected compared to non-infected patients suggests that RAS components play a role in gastric inflammation [11,18]. Moreover, AngII seems to aggravate ulceration in animal models [19].…”

“…The current concept of RAS evolved to local autonomous functions, from the classical systemic effect involving conversion of angiotensinogen (AGT) to angiotensin II (Ang II) facilitated by renin and angiotensin converting enzyme (ACE) [10]. Recent studies demonstrated the presence of RAS component in gastric mucosa to mediate cell proliferation, inflammation and fibrosis, promoting wound healing response, microvascular permeability, fluid and electrolyte transport as well as antral endocrine functions [10,11]. The question remains, however if genetic molecular predictors can be used for estimating the risk of GI events in a specific population.…”