The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

Nakagawa, Takayuki; Uyama, Rina; Ohashi, Emi; Takahashi, Tomoko; Hong, Sung Hyeol; Mochizuki, Manabu; Matsunaga, Satoru; Nishimura, Ryouhei; Sasaki, Nobuo

doi:10.1292/jvms.64.949

Cited by 11 publications

(17 citation statements)

References 24 publications

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“…This result is in agreement with the results described by Nakagawa et al in canine and feline mammary gland tumours [29]. Similarly, in woman breast cancer, Nakagoe et al did not find an association between sLe x expression and histological type of the carcinoma [20].…”

Section: Discussionsupporting

confidence: 92%

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Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

et al. 2007

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Background: Sialyl Lewis x (sLe x ) antigen is a carbohydrate antigen that is considered not only a marker for cancer but also implicated functionally in the malignant behaviour of cancer cells. Overexpression of sLe x is associated with enhanced progression and metastases of many types of cancer including those of the mammary gland. Canine mammary tumours can invade and give rise to metastases via either lymphatic or blood vessels.

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Section: Discussionsupporting

confidence: 92%

“…Little is known about the expression of sLe x in canine tumours. To the best of our knowledge only the study of Nakagawa et al describe the expression of sLe x in canine and feline mammary gland tumours [29], but no significant correlation between the expression of sLe x and prognosis has been described in canine or feline tumours.…”

Section: Introductionmentioning

confidence: 99%

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

et al. 2007

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“…34 In dogs, recent reports have confirmed that sialyl Lewis carbohydrates are detectable in canine malignant mammary tumors and that elevated levels of sLe x expression in these cancers are associated with lymph node metastases. 21,27 To our knowledge, this study is the first report of the detection of C2-O-sLe x carbohydrates in canine gastric carcinomas.…”

Section: Discussionmentioning

confidence: 71%

“…21,23,27,28 In humans, the sLe x antigen on leukocytes functions as a ligand for the selectin family of cell adhesion molecules and mediates the binding of leukocytes to vascular endothelium at sites of inflammation. These selectin-binding interactions are greatly enhanced when leukocytes display sLe x on a core 2 branched O-glycan carbohydrate structure (C2-O-sLe x ).…”

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confidence: 99%

The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

2010

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Sialyl Lewis x-modified core 2 branched O-glycans (C2-O-sLe x ) on human leukocytes mediate much higher-affinity adhesion to selectins on activated vascular endothelium than does sialyl Lewis x on other structures. In some canine and human carcinomas, high expression of sLe x -decorated carbohydrates has been associated with metastasis and, in humans, a poor prognosis, but detection in canine gastric carcinomas is unreported. The authors hypothesized that these carbohydrates are highly expressed in more malignant types of canine gastric carcinomas, they promote metastasis, and they are associated with a poorer prognosis for dogs. The objectives were to determine the presence and importance of C2-O-sLe x expression in canine gastric carcinomas. Routine histological sections of 16 canine gastric carcinomas were categorized on the basis of 3 classification schemes: World Health Organization, Lauren, and Goseki. Serial sections were stained with antibodies directed against C2-O-sLe x (CHO-131 monoclonal antibody), cytokeratin (Lu-5 monoclonal antibody), and stains to detect neutral and acid mucins (periodic acid-Schiff and alcian blue). Whereas normal gastric mucosal epithelial cells were negative for C2-O-sLe x , 56% of the tumors examined were positive for C2-O-sLe x . Importantly, the majority of more poorly differentiated tumor types had more numerous and larger intensely stained areas of C2-O-sLe x expression compared with moderate to well-differentiated tumor types. Signet ring-type carcinomas had markedly higher distribution and intensity of periodic acid-Schiff and alcian blue staining than did tubular carcinomas. These findings suggest that C2-O-sLe x is a tumor-associated antigen that may play a role in the invasiveness and metastatic potential of certain types of canine gastric carcinomas.

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“…Several clinical studies on human cancers have shown the relationship between sLe(x) expression on tumors and their progression and metastasis [6,7,19]. In studies on CMGTs, various levels of sLe(x) expression were observed on CMGT tissues, while no sLe(x) expressions was observed on normal tissues, and the relationship between the expression of sLe(x) and distant metastasis was not clear [18,24].…”

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confidence: 99%

Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X

Nakagawa

Endo

Watanabe

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The adhesional function to blood vessel endothelial cells was evaluated in 2 cultured canine mammary gland tumor cells, CHMp and CHMm, derived from the primary and the metastatic lesion of the same patient. They exhibited negative (CHMp) and positive (CHMm) expression of sialyl Lewis X [sLe(x)], respectively. In the cell adhesion assay, the number of attached CHMm cells to human umbilical vein endothelial cells which expressed activated E-selectin was significantly increased. The process of cell-cell adhesion under flow conditions was observed only in CHMm cells. CHMm cells were revealed to have the adhesional ability to blood vessel endothelial cells and this adhesional ability was thought to be induced by the sLe(x)-E-selectin binding.KEY WORDS: E-selectin, mammary gland tumor, sLe(x).

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The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

Cited by 11 publications

References 24 publications

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X

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The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

Cited by 11 publications

References 24 publications

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

The Novel Carbohydrate Tumor Antigen C2-O-sLexIs Upregulated in Canine Gastric Carcinomas

Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X

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The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas