The expression of vascular endothelial growth factor and its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bovine uterus during the sexual cycle and their correlation with serum sex steroids

Sağsöz, Hakan; Saruhan, Berna Güney

doi:10.1016/j.theriogenology.2011.01.012

Cited by 29 publications

(18 citation statements)

References 48 publications

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“…Regardless the mechanism, RU486 was able to selectively inhibit VEGF expression, triggered by hCG, within theca compartment of periovulatory follicles. This inhibitory effect may be a consequence of the antiprogesterone action of RU486, as reported in bovine [69], human [70] and mink [71], and in another reproductive tissue such as endometrium [37], [38], [42], [72] exposed to low concentrations of P 4 . As a consequence, the RU486 follicles displayed an incomplete development of the blood vessel network.…”

Section: Discussionmentioning

confidence: 76%

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

et al. 2014

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BackgroundThe success of ovarian follicle growth and ovulation is strictly related to the development of an adequate blood vessel network required to sustain the proliferative and endocrine functions of the follicular cells. Even if the Vascular Endothelial Growth Factor (VEGF) drives angiogenesis before ovulation, the local role exerted by Progesterone (P4) remains to be clarified, in particular when its concentration rapidly increases before ovulation.AimThis in vivo study was designed to clarify the effect promoted by a P4 receptor antagonist, RU486, on VEGF expression and follicular angiogenesis before ovulation, in particular, during the transition from pre to periovulatory follicles induced by human Chorionic Gonadotropins (hCG) administration.Material and MethodsPreovulatory follicle growth and ovulation were pharmacologically induced in prepubertal gilts by combining equine Chorionic Gonadotropins (eCG) and hCG used in the presence or absence of RU486. The effects on VEGF expression were analyzed using biochemical and immunohistochemical studies, either on granulosa or on theca layers of follicles isolated few hours before ovulation. This angiogenic factor was also correlated to follicular morphology and to blood vessels architecture.Results and ConclusionsVEGF production, blood vessel network and follicle remodeling were impaired by RU486 treatment, even if the cause-effect correlation remains to be clarified. The P4 antagonist strongly down-regulated theca VEGF expression, thus, preventing most of the angiogenic follicle response induced by hCG. RU486-treated follicles displayed a reduced vascular area, a lower rate of endothelial cell proliferation and a reduced recruitment of perivascular mural cells. These data provide important insights on the biological role of RU486 and, indirectly, on steroid hormones during periovulatory follicular phase. In addition, an in vivo model is proposed to evaluate how periovulatory follicular angiogenesis may affect the functionality of the corpus luteum (CL) and the success of pregnancy.

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Section: Discussionmentioning

confidence: 76%

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

et al. 2014

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“…All steps were carried out following the procedure described by Sağsöz and Saruhan. 26 Tissue sections were incubated with the GPx-1 (abcam-ab22604) (1:400) and insulin (abcam ab-181547) (1:1000) primary antibodys overnight at 4 0 C. Finally, to visualize the reactions, the sections were reacted for 5-15 min with diaminobenzidine (DAB) for GPx-1 and insulin staining. After the development of the DAB reactions, the sections were counterstained with Gill's Hematoxylin.…”

Section: Immunohistochemistry (Ihc) Stainingmentioning

confidence: 99%

Protective Effects of Bryonia multiflora Extract on Pancreatic Beta Cells, Liver and Kidney of Streptozotocin-Induced Diabetic Rats: Histopathological and Immunohistochemical Investigations

Uyar¹,

Yaman²,

Keleş³

et al. 2017

IJPER

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Objective: In this study, the ameliorative potential and antioxidant capacity of treated with different doses of Bryonia multiflora extract (BE) was investigated using histopathological and immunohistochemical changes in pancreatic beta cells, liver and kidney tissues of streptozotocin (STZ)-induced diabetic rats. Material and Methods: A total of forty-two healthy adult Wistar albino male rats were divided randomly into six groups as Control (C); Diabetes mellitus (DM); DM+Akarboz 20 mg/kg; DM+100 mg/kg BE extract (BE1); DM+200 mg/kg BE extract (BE2); DM+400 mg/kg BE extract (BE3). Experimental diabetes was established by a single-dose [45 mg/kg, intra-peritoneal (i.p)] STZ injection. Essential dosages of BE extracts and Akarboz were applied with gastric gavage for 21 day. Blood glucose levels were recorded throughout the all experiment period. Results: Histopathological studies showed that hepatorenal and pancreatic protection by depending on the dose level of BE extracts was further supported by the almost normal histology in DM+ BE extract-treated group as compared to the degenerative changes such as disorder of architectural structure, inflammatory cell infiltration, hydropic degeneration and necrosis in pancreas, liver and kidney tissues of STZ-treated rats. Immunohistochemical investigation revealed that STZ-induced degenerative changes in beta-cells in the pancreas of the diabetic rats has reduced insulin immunoreactivity. On the other hand, insulin immunoreactivity in the β cells of pancreas of BE -treated diabetic rats has significantly increased. Additionally, Glutathione peroxidase 1 (GPx1) immunoreactivity was lower in the tissues of diabetic rats (DM group) compared to the other groups. Conclusion: In conclusion, BE extract has a protective effect on tissue damage probably due to its antioxidant activity and possess the ability to regenerate β-cell in STZ-induced diabetic rats.

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“…Stromal and immune cells present in the uterine milieu regulate angiogenesis and vascular remodeling (Kuroda et al 2013). Many pro-angiogenic and anti-angiogenic modulators are expressed in the endometrium (Becker et al 2006, Saǧsöz andSaruhan 2011). Their expression varies during the cycle, and these factors often exhibit heterogeneous localization.…”

Section: Physiological and Pathological Angiogenesis In The Uterusmentioning

confidence: 99%

Is adenomyosis a problem in reproduction and fertility?

Korzekwa

Łupicka

Socha

et al. 2014

Polish Journal of Veterinary Sciences

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Adenomyosis is defined as the presence of glandular foci beside the endometrium of uterus: in the myometrium and/or perimetrium depending on the progress of the disorder. So far, adenomyosis has been diagnosed in women and rodents, and studies conducted on cows have been rare. In this review we: (1) summarize the knowledge regarding adenomyosis, (2) compare the symptoms and aetiopathology between women and cows, (3) describe angiogenic uterine processes related to adenomyosis development and (4) outline the influence of adenomyosis on proper fertility processes in cattle (conception and fertility rates).

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The expression of vascular endothelial growth factor and its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bovine uterus during the sexual cycle and their correlation with serum sex steroids

Cited by 29 publications

References 48 publications

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

Protective Effects of Bryonia multiflora Extract on Pancreatic Beta Cells, Liver and Kidney of Streptozotocin-Induced Diabetic Rats: Histopathological and Immunohistochemical Investigations

Is adenomyosis a problem in reproduction and fertility?

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