The expressions of some metabolic hormones (leptin, ghrelin and obestatin) in the tissues of sheep tongue

Abstract

In this study, we aimed to observe the localization and expression of peptide hormones—leptin, ghrelin and obestatin—in the sheep tongue by immunohistochemistry. For that purpose, tongues of ten adult sheep were used. Leptin expression of moderate intensity was observed in the basal and parabasal epithelial cells of the luminal epithelium, and leptin was strongly expressed in the taste buds of the circumvallate and fungiform papillae and in von Ebner's glands. Ghrelin was primarily expressed in some of the skeletal muscle cells and the smooth muscle cells of the middle layer of blood vessels. A strong expression was observed in the epithelial cells lining the base of the groove surrounding the circumvallate papillae. Obestatin expression was particularly strong in the epithelial cells of the salivary ducts. It was also stronger in the von Ebner's glands than in the seromucous glands. Leptin, ghrelin and obestatin were shown to be produced at varying levels in different cell types, including epithelial, stromal and skeletal muscle cells, as well as in ganglion neurons, neural plexuses and blood vessels in the sheep tongue. Cellular localization and expression of these peptide hormones have not been investigated in many species including sheep.

Sıçanların Meme Dokusunda Gebelik, Laktasyon ve İnvolusyon Periyodları Süresince Ghrelin, Obestatin ve Leptin Dağılımı

“…Leptin, ghrelin ve obestatin immünoreaktiviteleri, boyamanın yoğunluğuna dayalı yarı kantitatif skorlama yöntemi kullanılarak ölçüldü (18). Bu yöntemde immunohistokimyasal skorlama (-) negatif; (+) zayıf; (++) orta veya (+++) güçlü olarak değerlendirildi.…”

Immunohistochemical distributions of leptin, ghrelin and obestatin hormones in bull and ram abomasum

“…Leptin is a 16 kDa single‐chain polypeptide hormone that is mainly synthesized in the white adipose tissue (Karakoç et al., 2019; Sağsöz et al., 2020). Leptin has been detected in a variety of tissue types such as placenta, mammary glands, skeletal muscle, hypothalamus and pituitary gland, using immunocytochemistry and molecular biology methods (Akbalık, 2019).…”

“…Leptin has been detected in a variety of tissue types such as placenta, mammary glands, skeletal muscle, hypothalamus and pituitary gland, using immunocytochemistry and molecular biology methods (Akbalık, 2019). Leptin plays central and peripheral roles in a wide range of metabolic activities including feed consumption and digestion, energy expenditure, thermogenesis, and the regulation of carbohydrate‐fat accumulation (Sağsöz et al., 2020). Recent studies have shown that leptin inhibits insulin secretion.…”

“…It is synthesized in many tissues such as the stomach, small intestine, spleen, liver, breast, hypothalamus and pituitary gland. It has also been detected in milk, plasma and serum (Sağsöz et al., 2020). Obestatin is a ghrelin‐related peptide that has the opposite effect of ghrelin.…”

“…Ghrelin is a 28 amino acid lipopeptide hormone secreted mainly from the fundus and pylorica regions of the stomach (Karakoç et al, 2019). It has been reported that this hormone is also expressed in organs such as the duodenum, jejunum, ileum, colon, brain, salivary glands, thyroid gland, heart, pancreas, kidneys, testicles, lungs, placenta, gonads, immune system, mammary glands and even teeth (İlhan & Erdost, 2009, Sağsöz et al, 2020Özfiliz et al, 2011). Ghrelin is a powerful appetite-stimulating hormone.…”