There is a growing need for protein
for both feed and food in order
to meet future demands. It is imperative to explore and utilize novel
protein sources such as protein from leafy plant material, which contains
high amounts of the enzyme ribulose-1,5-biphosphate carboxylase/oxygenase
(RuBisCo). Leafy crops such as grasses and legumes can in humid climate
produce high protein yields in a sustainable way when compared with
many traditional seed protein crops. Despite this, very little RuBisCo
is utilized for foods because proteins in the leaf material has a
low accessibility to monogastrics. In order to utilize the leaf protein
for food purposes, the protein needs to be extracted from the fiber
rich leaf matrix. This conversion of green biomass to valuable products
has been labeled green biorefinery. The green biorefinery may be tailored
to produce different products, but in this Review, the focus is on
production of food-grade protein. The existing knowledge on the extraction,
purification, and concentration of protein from green biomass is reviewed.
Additionally, the quality and potential application of the leaf protein
in food products and side streams from the green biorefinery will
be discussed along with possible uses of side streams from the protein
production.