The extracellular adherence protein (Eap) of Staphylococcus aureus acts as a proliferation and migration repressing factor that alters the cell morphology of keratinocytes

Eisenbeis, Janina; Peisker, Henrik; Backes, Christian; Bur, Stephanie; Hölters, Sebastian; Thewes, Nicolas; Greiner, Markus; Junker, Christian; Schwarz, Eva C.; Hoth, Markus; Junker, Kerstin; Preissner, Klaus T.; Jacobs, Karin; Herrmann, Mathias; Bischoff, Markus

doi:10.1016/j.ijmm.2017.01.002

Cited by 12 publications

(6 citation statements)

References 68 publications

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“…Additionally, MRSA is a major cause of hospital-acquired infection of surgical wounds 14 . MRSA impair wound healing through secretion of virulence factors, such as the extracellular adherence protein Eap, which interfere with the proliferation and migration capacities of keratinocytes by altering their morphology and adhesive properties 15 . Furthermore, the planktonic and biofilm MRSA produce soluble products that have an adverse effect on the migration and viability of human fibroblasts which is crucial in wound healing process by inducing apoptosis 16 .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

Farghali

AbdElKader

AbuBakr

et al. 2019

Sci Rep

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Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicrobial properties of platelet-rich plasma (PRP) against various microorganisms enable its potential use as an alternative to conventional antibiotics. The present work was designed to evaluate the hypothesized antimicrobial activity of PRP against MRSA infected skin wounds. Six adult male dogs were divided equally into control and PRP groups. Unilateral circular full-thickness skin wounds were created then a MRSA suspension was injected locally. Treatment started at 1st week post infection with subcutaneous infiltration of autologous activated PRP every week in the PRP group and with topical application of clindamycin cream twice daily in the control group. PRP decreased wound size and significantly increased wound contractility and re-epithelization, as confirmed by histopathological and immunohistochemical findings. Also PRP treated group showed significant decrease in ROS and redox imbalance with over expression of the TNF-α and VEGFA genes that indicate angiogenesis and maximum antibacterial activity after three weeks. In conclusion, CaCl 2 -activated PRP exhibited antimicrobial activity against MRSA infection, which improved the infected wound healing re-epithelization and granulation tissue formation.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

Farghali

AbdElKader

AbuBakr

et al. 2019

Sci Rep

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“…Superantigens Enterotoxins (SE A-G, SEQ) Stimulation of large populations of T-cells (Pinchuk et al, 2010) Toxic shock syndrome toxin-1 (TSST-1) Stimulation of large populations of T-cells (Silversides et al, 2010) 2.iv. Miscellaneous proteins Chemotaxis inhibitory protein (CHIPS) Inhibition of chemotaxis of neutrophils (de Haas et al, 2004)Extracellular adherence protein (Eap) Inhibition of neutrophil migration and proliferation(Eisenbeis et al, 2017) Extracellular fibrinogen binding protein (Efb) Inhibition of complement activation and protection against phagocytosis(Ko et al, 2011)…”

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confidence: 99%

Role of staphylococci in mastitis in sheep

Vasileiou

Chatzopoulos

Sarrou

et al. 2019

Journal of Dairy Research

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Staphylococci have been isolated from various sites of the body of healthy sheep, as well as from many infections of those animals, the main one being mastitis. The objective of this review is to appraise the importance and significance of staphylococci in causing mastitis in ewes. The review includes a brief classification and taxonomy of staphylococci and describes the procedures for their isolation and identification, as well as their virulence determinants and the mechanisms of resistance to antibacterial agents. Various staphylococcal species have been implicated in staphylococcal mastitis and the characteristics of isolates are discussed with regards to potential virulence factors. Staphylococcal mastitis is explicitly described, with reference to sources of infection, the course of the disease and the relevant control measures. Finally, the potential significance of staphylococci present in ewes’ milk for public health is discussed briefly.

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“…Another S. aureus factor which may serve as binding partner for eDNA on the bacterial surface is extracellular adherence protein (Eap), a member of the “secretable expanded repertoire adhesive molecules” (SERAM) protein family (Chavakis et al, 2005 ). This basic protein is expressed by S. aureus in a growth phase-dependent manner (Joost et al, 2009 ), and secreted in large quantities into the extracellular milieu (Eisenbeis et al, 2017 ), from which it partially rebinds to the bacterial surface through the surface-bound neutral phosphatase (Nptase) and other yet unidentified cell wall molecules (Palma et al, 1999 ; Flock and Flock, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

Eisenbeis

Saffarzadeh

Peisker

et al. 2018

Front. Cell. Infect. Microbiol.

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The extracellular adherence protein (Eap) of Staphylococcus aureus is a secreted protein known to exert a number of adhesive and immunomodulatory properties. Here we describe the intrinsic DNA binding activity of this multifunctional secretory factor. By using atomic force microscopy, we provide evidence that Eap can bind and aggregate DNA. While the origin of the DNA substrate (e.g., eukaryotic, bacterial, phage, and artificial DNA) seems to not be of major importance, the DNA structure (e.g., linear or circular) plays a critical role with respect to the ability of Eap to bind and condense DNA. Further functional assays corroborated the nature of Eap as a DNA binding protein, since Eap suppressed the formation of “neutrophil extracellular traps” (NETs), composed of DNA-histone scaffolds, which are thought to function as a neutrophil-mediated extracellular trapping mechanism. The DNA binding and aggregation activity of Eap may thereby protect S. aureus against a specific anti-microbial defense reaction from the host.

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The extracellular adherence protein (Eap) of Staphylococcus aureus acts as a proliferation and migration repressing factor that alters the cell morphology of keratinocytes

Cited by 12 publications

References 68 publications

Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

Role of staphylococci in mastitis in sheep

The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

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