The extracellular polysaccharide of Porphyridium sp.: an NMR study of lithium-resistant oligosaccharidic fragments

Gloaguen, Vincent; Ruiz, Gaël; Morvan, Henri; Mouradi-Givernaud, Aziza; Maës, Emmanuel; Krausz, Pierre; Strecker, Gérard

doi:10.1016/j.carres.2003.09.020

Cited by 58 publications

(33 citation statements)

References 29 publications

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“…The low neutral sugar content could be explained either by the presence of high levels of no sugar components either assuming that the absorption coefficient of glucose was inferior to this(ese) of constitutive monosaccharide(s) of these exopolymers. Our results verified the presence of carbohydrates in the different fractions depicted in literature as either polysaccharides [14,18,19,29] or proteoglycan by some strains of Rhodella [5]. A. platensis polysaccharidic fraction is depicted as a very complex structure of nine monosaccharides (Gal, Xyl, Glc, Fuc, Rha, Ara, Man, GalA, GlcA) by Trabelsi et al [31].…”

Section: Chemical and Physico-chemical Characterizations Of Exopolymesupporting

confidence: 93%

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A new tool to detect high viscous exopolymers from microalgae

Badel¹,

Callet²,

Laroche³

et al. 2010

J Ind Microbiol Biotechnol

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Microalgae are microorganisms often surrounded by a slime layer made of secreted polymeric substances sometimes including polysaccharides. These polysaccharides, weakly described in the literature, can constitute value-added molecules in several industrial areas. The aim of this article is to show that a new tool, the BioFilm Ring Test®, can be used to detect viscous microalgal exopolymers. Two red microalgal strains (Rhodella violacea and Porphyridium purpureum), one cyanobacterium (Arthrospira platensis) and their excreted polymeric fractions were studied. R. violacea and P. purpureum induced a positive response with the BioFilm Ring Test® contrary to A. platensis. Finally, the understanding of the fractions viscosity involvement in the BRT response was performed by a rheological study.

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Section: Chemical and Physico-chemical Characterizations Of Exopolymesupporting

confidence: 93%

“…[19], Chlorella vulgaris [24], Arthrospira platensis [29], Dixioniella grisea [1], Botryococcus braunii [15] or Rhodella violacea [5]. In all cases, polysaccharides or proteoglycans have been identified and some of them found industrial applications as those produced by Porphyridium species [13,34].…”

Section: Introductionmentioning

confidence: 98%

A new tool to detect high viscous exopolymers from microalgae

Badel¹,

Callet²,

Laroche³

et al. 2010

J Ind Microbiol Biotechnol

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“…From an anionic polymer extracted from the bound fraction of the polysaccharide synthesized by Porphyridium sp., two oligosaccharides different from the aldobiuronic acid were isolated. This bulk polymer resulted composed by xylose, glucose, galactose and galacturonic acid (Gloaguen et al 2004 ).…”

Section: Characteristics and Composition Of Epss From Microalgae And mentioning

confidence: 99%

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Rossi

Philippis

2016

The Physiology of Microalgae

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Aims and ScopeApplied Phycology, the practical use of algae, encompasses a diverse range of fields including algal culture and seaweed farming, the use of algae to produce commercial products such as hydrocolloids, carotenoids and pharmaceuticals, algae as biofertilizers and soil conditioners, the application of algae in wastewater treatment, renewable energy production, algae as environmental indicators, environmental bioremediation and the management of algal blooms. The commercial production of seaweeds and microalgae and products derived there from is a large and well established industry and new algal species, products and processes are being continuously developed.The aim of this book series, Developments in Applied Phycology, is to present state-ofthe-art syntheses of research and development in the field. Volumes of the series will consist of reference books, subject-specific monographs, peer reviewed contributions from conferences, comprehensive evaluations of large-scale projects, and other booklength contributions to the science and practice of applied phycology.

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“…Outra aplicação mais recente do uso deste íon é a de fragmentar polissacarídeos extracelulares ou da parede celular, auxiliando nas investigações estruturais desses polímeros (Petrus et al 1995;Flores et al 2000;Fransen et al 2000;Gloaguen et al 1999;2004). É sabido da química do Li + que é um íon de pequeno raio iônico e que requer um número relativamente grande de moléculas de água no processo de solvatação; entretanto, não é muito clara sua ação como elemento desorganizador da estrutura de polissacarídeos.…”

Section: Introductionunclassified

Efeito caotrópico do íon lítio na permeabilidade da cápsula polissacarídica da microalga Ankistrodesmus gracilis (Reinsch) Korsikov (Chlorophyceae)

et al. 2006

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RESUMO -(Efeito caotrópico do íon lítio na permeabilidade da cápsula polissacarídica da microalga Ankistrodesmus gracilis (Reinsch) Korsikov (Chlorophyceae)). O objetivo deste trabalho foi investigar o efeito caotrópico do íon Li + na cápsula polissacarídica da microalga colonial Ankistrodesmus gracilis. Esta microalga possui uma cápsula extensa e contínua que envolve completamente as células. Neste estudo, foram utilizados a técnica de Ressonância Paramagnética Eletrônica (RPE) e o marcador de spin -Tempo (2,2,6,6-tetrametilpiperidine-1-oxil), de natureza não reativa. A técnica de RPE é utilizada para monitorar o tempo de difusão dos marcadores de spin através da cápsula polissacarídica, parede e membrana celular, até o interior da célula, onde eles perdem suas propriedades paramagnéticas. O íon Li + utilizado tem a propriedade de provocar a desorganização estrutural da cápsula e, em conseqüência, a modificação do tempo de difusão do marcador de spin. Observou-se que os tempos de decaimento do sinal paramagnético nas células tratadas com Li + são sempre menores do que nas células não expostas ao Li + e que os tempos de difusão diminuem com o aumento da concentração de LiCl . As fotomicrografias das algas mostram que a partir da concentração de LiCl 1,0 M, as cápsulas são totalmente retiradas, resultando em células que se aglutinam formando grumos. Também foi constatada a morte das células acima quando expostas a concentração de LiCl acima de 1,0 M. Palavras-chave : cápsula polissacarídica, Ankistrodesmus gracilis, LiCl , marcador de spin e tempo de permeação ABSTRACT -(Chaotropic effect of lithium íon on permeability of polysaccharide capsule of the microalga Ankistrodesmus gracilis (Reinsch) Korsikov (Chlorophyceae)). The aim of this study was to investigate the role of chaotropic effect of Li+ on the polysaccharide sheath of the microalga Ankistrodesmus gracilis (Chlorophyceae, Chlorococales) in the selective permeability and transport of molecules in the interior of the cell. The colonial microalga Ankistrodesmus gracilis has a relatively large and continuous mucilaginous sheath. The Electron Paramagnetic Resonance (EPR) was applied using the spin label-Tempo 2,2,6,6-tetramethylpiperidine-1-oxyl of non-reactive nature to study its diffusion coefficients across the polysaccharide envelope. The EPR technique is used to monitor the spin labels diffusion time across the polysaccharide capsule, cell wall and membrane into the interior of the cell, where they lose their paramagnetic properties. The lithium ion (Li+) has the property to structurally disorganize the polysaccharide capsule and the spin label diffusion changes producing different spin label time decay. The time decays of cells subjected to Li+ treatment are faster than the cells without this ion usage and the decay time decreases with increasing Li+ concentration. The photomicrographies show the complete removal of the cell capsule from LiCl 1,0 M upward and as a result, the algal cells agglutinate producing cell clumps, and the algal cells are clearl...

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The extracellular polysaccharide of Porphyridium sp.: an NMR study of lithium-resistant oligosaccharidic fragments

Cited by 58 publications

References 29 publications

A new tool to detect high viscous exopolymers from microalgae

A new tool to detect high viscous exopolymers from microalgae

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Efeito caotrópico do íon lítio na permeabilidade da cápsula polissacarídica da microalga Ankistrodesmus gracilis (Reinsch) Korsikov (Chlorophyceae)

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