The extract ofCinnamomum cassiatwigs inhibits adipocyte differentiation via activation of the insulin signaling pathway in 3T3-L1 preadipocytes

Abstract

Context: Obesity is associated with a number of diseases with metabolic abnormalities such as type 2 diabetes (T2D). Medicinal plants have been widely used for the treatment of obesity and related complications. Objective: In this study, we investigated the antidiabetic properties of the extract of twigs of Cinnamomum cassia Blume (Lauraceae) (Cinnamomi Ramulus; CR) in 3T3-L1 murine preadipocytes. Materials and methods: 3T3-L1 cells were differentiated into adipocytes for 3 d in insulinconditioned medium and then treated with CR extract at concentrations of 100 and 500 mg/mL for 6 d. Adipocyte differentiation was measured by Oil Red O staining, and the expression of master transcription factors, peroxisome proliferator-activated receptor-gamma (PPARg), CCAAT/enhancer binding protein-alpha (C/EBPa), and sterol regulatory element binding protein-1c (SREBP-1c), and lipid metabolism factors were investigated by reverse transcriptionpolymerase chain reaction (RT-PCR). The activation of the AMP-activated protein kinase (AMPK)/ insulin signaling pathway was assessed by western blot analysis. Results: CR extract significantly reduced lipid accumulation and down-regulated the expression of PPARg, C/EBPa, and SREBP-1c in 3T3-L1 adipocytes. CR extract also suppressed the expression of fatty acid synthase (FAS), acyl-CoA synthase, and perilipin. Moreover, CR extract markedly up-regulated the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC). In addition, CR extract effectively increased the expression levels of glucose transporter-4 (GLUT-4), phosphatidylinositol 3-kinase (PI3K), and insulin receptor substrate-1 (IRS-1) in 3T3-L1 adipocytes. Discussion and conclusion: These results suggest that CR extract may have therapeutic potential as a natural agent for the improvement of T2D via regulation of the insulin-dependent signaling pathway. Keywords3T3-L1 Adipocyte, Cinnamomi Ramulus, lipid metabolism, type 2 diabetes History

Phytochemicals as novel agents for the induction of browning in white adipose tissue

“…Cinnamon decreased lipid accumulation in vitro in 3T3-L1 preadipocytes and the expression of adipogenic transcription factors PPARγ, C/EBPα and SREBP-1c during adipocyte differentiation [102]. In a dose-dependent manner, cinnamaldehyde also decreased visceral fat deposition, partly mediated by the activation of interscapular BAT, as evidenced by the upregulation of UCP1 expression levels, in high fat and high sucrose diet-fed mice [97].…”

A sensitive UPLC–MS/MS method for simultaneous determination of polyphenols in rat plasma: Application to a pharmacokinetic study of dispensing granules and standard decoction of Cinnamomum cassia twigs

“…It is commonly used in the treatment of cold fever. Modern pharmacological studies revealed that CR could not only exhibit antineuroinflammatory (Yang, Cheng, Yang, Wang, & Du, 2017), antidiabetic (Han, Jung, Bae, Kang, & Park, 2013) and anti-gastric lesions effects (Joohee, Je-Hyuk, Ki Hwan, & Choon-Sik, 2011), but also have anti-bacterial (Liu et al, 2007) and chemopreventive (Park, Lee, Oh, Bae, & Kim, 2005) functions. The main bioactive components of CR are phenolics such as cinnamic acid (Wu, He, Xu, Bi, & Li, 2014).…”

Antioxidant and Antiadipogenic Activities of Galkeun‐Tang, a Traditional Korean Herbal Formula

“…Pueraria lobata extract also inhibited tert -butyl hydroperoxide-induced ROS generation [ 37 ]. An extract of Cinnamomum cassia twigs inhibited adipocyte differentiation via activation of the insulin signaling pathway in 3T3-L1 preadipocytes [ 38 ]. Ephedra sinica extract exerted antioxidant effects by accelerating free radical scavenging activity and oxidant reducing power [ 39 ].…”