The Extraction and High Antiproliferative Effect of Anthocyanin from Gardenblue Blueberry

Abstract

Blueberries are rich in flavonoids, anthocyanins, phenolic acids, and other bioactive substances. Anthocyanins are important functional components in blueberries. We collected 65 varieties of blueberries to investigate their nutritional and functional values. Among them, Gardenblue had the highest anthocyanin content, with 2.59 mg/g in fresh fruit. After ultrasound-assisted solvent extraction and macroporous resin absorption, the content was increased to 459.81 mg/g in the dried powder. Biological experiments showed that Gardenblue anthocyanins (L1) had antiproliferative effect on cervical cancer cells (Hela, 51.98 μg/mL), liver cancer cells (HepG2, 23.57 μg/mL), breast cancer cells (MCF-7, 113.39 μg/mL), and lung cancer cells (A549, 76.10 μg/mL), and no apparent toxic effects were indicated by methyl thiazolyl tetrazolium (MTT) assay, especially against HepG2 cells both in vitro and in vivo. After combining it with DDP (cisplatin) and DOX (doxorubicin), the antiproliferative effects were enhanced, especially when combined with DOX against HepG2 cells; the IC50 value was 0.02 μg/mL. This was further evidence that L1 could inhibit cell proliferation by inducing apoptosis. The detailed mechanism might be L1 interacting with DNA in an intercalation mode that changes or destroys DNA, causing apoptosis and inhibiting cell proliferation. The findings of this study suggest that L1 extract can be used as a functional agent against hepatoma carcinoma cells.

Polyphenols fromPrunus mume: extraction, purification, and anticancer activity

“…According to our previous report, Prunus mume polyphenol extract may interact with DNA in an intercalation mode, which can change or destroy DNA, thus causing apoptosis and the inhibition of cell proliferation activity. 49,50 However, the result is preliminary and needs further proof. The deeper mechanism, even the mechanism of action of the drugs used and how the effect of the extract in combination with doxorubicin and cisplatin is enhanced, are worth evaluating to better understand combination drugs and explore the possible mechanisms of the anticancer effect of Prunus mume polyphenol extract.…”

The Therapeutic Effects of Blueberry-Treated Stem Cell-Derived Extracellular Vesicles in Ischemic Stroke

“…The blueberries used in this study are classified as anthocyanins. Anthocyanins are a type of secondary metabolite in plants, produced through the phenylpropionate and flavonoid synthesis pathways [ 82 ]. Blueberries are known to reduce the damage caused by ischemic stroke.…”

A Review with a Focus on Vaccinium-Berries-Derived Bioactive Compounds for the Treatment of Reproductive Cancers

“…The antiproliferative effects of ACNs from Gardenblue blueberries have been confirmed in a recent in vitro experiment. The results showed that ACNs in combination with chemotherapeutic drugs (cisplatin and doxorubicin) inhibit the growth of CC cells at a concentration of 51.98 µg/mL; however, the mechanisms behind this process have not been documented [43]. A significant inhibition of OC and CC cell proliferation in a dose-dependent manner was reported in in vitro experiments following treatment with blueberry juice.…”