THE EXTRACTION OF NITROGENOUS CONSTITUENTS FROM PLANT CELLS¹

The Extraction of Plant Tissue Fluids and Their Utility in Physiological Studies

“…Methods for the complete extraction of leaf cell proteins have been proposed by CHIBNALL (1) and by TOTTINGHAM and co-workers (8). These, …”

Preparation of Aqueous Extracts of Soluble Nitrogen From Plant Tissues

“…In the preparation of an extract by the method recommended by TOT-TINGHAM et al (9), a 50-gm. or 100-gm.…”

“…It has been shown by CHIBNALL (3), and by TOTTINGHAM et al (9), that the drying of plant tissues before extraction changes the proportional distribution of nitrogen among the nitrogenous constituents. Accordingly, only aqueous extracts from fresh plant tissues have been used by CHIBNALL (2), VICKERY and PUCHER (10), NIGHTINGALE (7), and MURNEEK (6) in studies on the nitrogen metabolism of plants.…”

“…(9) and NIGHTINGALE (7) ground the plant tissues in a mortar with the aid of sand. The preparation of extracts by this method is attended with much labor and requires considerable time.…”

Biochemical Methods for the Study of Nitrogen Metabolism in Plants

“…The preparation, alone, of plant juice for analysis of nitrogen constituents has been the object of a number of investigations (1,4,19), but at the present time there is no generally used or accepted method. This is partially due to varying constituents in different types of tissue, but it is more directly due to the inadequacy of these methods for giving extracts or juices which can be readily analyzed.…”