In a preliminary report, DEXTER. TOTTINGHAMI and GRABER (3)

The injury of plants occasioned by winter conditions has been a subject of great interest and importance to plant breeders and agriculturists. The development of hardy strains has been, and is, one of the principal means of comiibating losses caused by injury from cold, although cultural methods, as well, lhave been adapted to help the plant survive the rigors of its climatic environiment. To test this hypothesis, alfalfa roots of three varieties of known hardiness (Grimm, Utah Common, and Hairy Peruvian) were frozen under con- Recog

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The iron division precipitate is treated directly with nitric acid and potassium chlorate to remove the manganese. (At this point phosphates may be removed if present.) Iron is removed by adding a large excess of ammonium hydroxide. The filtrate from the iron precipitate is evaporated until Only a faint odor of ammonia remains and nickel is tested for by adding dimethyl glyoxime directly to V6 of this solution, no adjustment of hydrogen ion being necessary.The remainder of the solution is further evaporated to a sirup and transferred to a test-tube; enough water is then added to dissolve the precipitated salts, and cobalt is tested for by a modified Vogel's test; 3 cc. of 10% potassium thiocyanate solution is added, followed by 3 cc. of a mixture of amyl alcohol and ether (1 vol. : 3 vol.) and the tube gently shaken, Cobalt causes the ether layer to become blue-green. If the color is not distinct, the careful addition of a few drops of 12 N hydrochloric acid to the ether layer will give the blue-green color if cobalt is present, or a straw-colored or colorless layer, if it is absent. This test is extremely sensitive and can be applied to the original solution if iron is absent.The writer wishes to thank Professor J. H. Reedy of the University of Illinois and Professor J. F. G. Hicks of the University of Nevada for testing the above method with their students.